[논문]목향 추출물의 항비만 활성 효과

윤태숙; 성윤영; 장자영; 양원경; 지윤의; 김호경

목향 추출물의 항비만 활성 효과
Anti-obesity Activity of Extract from Saussurea lappa 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.18 no.3, 2010년, pp.151 - 156

윤태숙 (한국한의학연구원 한약자원연구센터) , 성윤영 (한국한의학연구원 한약자원연구센터) , 장자영 (한국한의학연구원 한약자원연구센터) , 양원경 (한국한의학연구원 한약자원연구센터) , 지윤의 (한국한의학연구원 한약자원연구센터) , 김호경 (한국한의학연구원 한약자원연구센터)

Abstract ▼ AI-Helper

Obesity has become one of the main public health problems. Saussurea lappa (Asteraceae), syn Aucklandia lappa and Saussurea costus, is a well-known herbal medicine that has been used for treating various ailments, such as inflammatory and gastrointestinal diseases. The present study examined the anti-obesity effect of S. lappa extract (SLE) in 3T3-L1 adipocytes and high fat diet (HFD)-induced obese mouse model. SLE significantly inhibited the differentiation from preadipocytes to adipocytes of cultured 3T3-L1 in dose-dependent manner. In addition, SLE significantly decreased the body weight gain and the food efficiency ratio of mice fed HFD during 9 weeks. Further study must be performed for the pharmacological mechanism and safety of SLE as well as the identification of active compound in SLE. Our results revealed that S. lappa suppresses the adipogenesis in cultured cells and the obesity in rodent models. Therefore, S. lappa may be useful toward the development of new potent anti-obesity drugs.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Our study focuses on the anti-obesity activity of the root of S. lappa with respect to developing its medicinal applications and potential uses. In the present study, we investigated that the effect of S.

제안 방법

Rodent diet D12492 (Research diet, New Brunswick, NJ, USA), providing 60% of energy as fat, 20% as protein, and 20% as carbohydrate, was used to induce obesity for high fat diet (HFD) group. After 6 weeks of dietary manipulation with HFD, SLE was orally administered at doses of 200 mg/kg/day for consecutive 9 weeks to the mice in HFD+SLE group. The concentration of SLE used in these experiments is a minimal dosage with effective as determined from the preliminary dose-response experiment(data not shown).
, 2007). During the experiment period, the amounts of body weight and food intake were monitored twice every week. The body weight was gradually increased with time, and the body weight of HFD-fed mice group became higher than that of normal diet (ND)-fed mice group for 6 weeks.
, 1994). To investigate the effect of SLE on the differentiation to adipocyte, 3T3-L1 preadipocytes were treated with various concentrations of SLE during differentiation. SLE of testing concentrations did not affect the viability (data not shown).

대상 데이터

3T3-L1 preadipocytes were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA) and maintained with DMEM containing 10% heat-inactivated CS, 100 units/㎖ penicillin, and 100 ㎍/㎖ streptomycin at 37℃ in a humidified atmosphere of 5% CO₂.
Four-week-old male C57BL/6J mice of 19-20 g were obtained from Daehan Biolink Co. (Eumsung, Korea). The animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of Korea Institute of Oriental Medicine.
lappa (Asteraceae) were purchased from Omniherb (Oriental drug store, Yeongcheon, Korea) and were authenticated based on microscopic characteristics according to the ‘Classification and Identification Committee of the Korea Institute of Oriental Medicine’. This committee was composed of nine experts in the fields of plant taxonomy, botany, pharmacognosy, and herbology. In addition, a voucher specimen (KIOM008174) was deposited at the herbarium of the Center of Herbal Resources Research at Korea Institute of Oriental Medicine (Daejeon, Korea).

데이터처리

All data were expressed as mean ± S.E. Statistical significance was analyzed by Student's t-test and ANOVA using SPSS ver.14 (SPSS Inc., Chicago, IL, USA).

이론/모형

The differentiation, as evaluated by the expression of adipogenic markers and appearance of lipid droplets, was completed at day 8. The cytotoxicity test was performed to examine the effect of SLE on the viability of 3T3-L1 cells using MTT assay, which is based on the cleavage of tetrazolium salt by mitochondrial dehydrogenase in viable cells (Carmichael et al., 1987).

성능/효과

In conclusion, the present study reports for the first time that S. lappa has anti-obesity activity in 3T3-L1 adipocytes and high fat diet-induced obese mice. Thus, S.
During the experiment period, the amounts of body weight and food intake were monitored twice every week. The body weight was gradually increased with time, and the body weight of HFD-fed mice group became higher than that of normal diet (ND)-fed mice group for 6 weeks. Fig.

후속연구

To clarify pharmacological mechanisms and safety of SLE, the further investigations must be performed. Also, the intensive researches need to be performed for the discovery of the new active components in SLE responsible for anti-obesity effect.
To clarify pharmacological mechanisms and safety of SLE, the further investigations must be performed. Also, the intensive researches need to be performed for the discovery of the new active components in SLE responsible for anti-obesity effect.

참고문헌 (34)

Bhathena SJ and Velasquez MT. (2002). Beneficial role of dietary phytoestrogens in obesity and diabetes. American Journal of Clinical Nutrition. 76:1191-1201.

상세보기
Bray GA and Tartaglia LA. (2000). Medicinal strategies in the treatment of obesity. Nature. 404:672-677.

상세보기
Buettner R, Scholmerich J and Bollheimer LC. (2007). High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity. 15:798-808.

상세보기
Carmichael J, DeGraff WG and Gazdar AF. (1987). Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Research. 47:936-941.

상세보기
Chen HC, Chou CK, Lee SD, Wang JC and Yeh SF. (1995). Active compounds from Saussurea lappa Clarks that suppress hepatitis B virus surface antigen gene expression in human hepatoma cells. Antiviral Research. 27:99-109.

상세보기
Chen SF, Li YQ and He FY. (1994). Effect of Saussurea lappa on gastric functions. Zhongguo Zhong Xi Yi Jie He Za Zhi. 14:406-408.
Cho JY, Kim AR, Jung JH, Chun T, Rhee MH and Yoo ES. (2004). Cytotoxic and pro-apoptotic activities of cynaropicrin, a sesquiterpene lactone, on the viability of leukocyte cancer cell lines. European Journal of Pharmacology. 492:85-94.

상세보기
Cho JY, Park J, Yoo ES, Baik KU, Jung JH, Lee J and Park MH. (1998). Inhibitory effect of sesquiterpene lactones from Saussurea lappa on tumor necrosis factor-alpha production in murine macrophage-like cells. Planta Medica. 64:594-597.

상세보기
Feve B. (2005). Adipogenesis: cellular and molecular aspects. Best Practice & Research Clinical Endocrinology & Metabolism. 19:483-499.

상세보기
Friedman JM and Halaas JL. (1998). Leptin and the regulation of body weight in mammals. Nature. 395:763-770.

상세보기
Jeong SJ, Itokawa T, Shibuya M, Kuwano M, Ono M, Higuchi R and Miyamoto T. (2002). Costunolide, a sesquiterpene lactone from Saussurea lappa, inhibits the VEGFR KDR/Flk-1 signaling pathway. Cancer Letters. 187:129-133.

상세보기
Kim JK, So H, Youn MJ, Kim HJ, Kim Y, Park C, Kim SJ, Ha YA, Chai KY, Kim SM, Kim KY and Park R. (2007). Hibiscus sabdariffa L. water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway. Journal of Ethnopharmacology. 114:260-267.

상세보기
Ko SG, Kim HP, Jin DH, Bae HS, Kim SH, Park CH and Lee JW. (2005). Saussurea lappa induces G2-growth arrest and apoptosis in AGS gastric cancer cells. Cancer Letters. 220:11- 19.

상세보기
Kopelman PG. (2000). Obesity as a medical problem. Nature. 404:635-643.

상세보기
Lagra F, Karastergiou K, Delithanasis I, Koutsika E, Katsikas I and Papadopoulou-Zekeridou P. (2004). Obesity and colorectal cancer. Techniques in Coloproctology. 8(S):161-163.

상세보기
Lee GI, Ha JY, Min KR, Nakagawa H, Tsurufuji S, Chang IM and Kim Y. (1995). Inhibitory effects of Oriental herbal medicines on IL-8 induction in lipopolysaccharide-activated rat macrophages. Planta Medica. 61:26-30.

상세보기
Lee JH, Cho CW, Han XF, Hwang JY, Kang MJ, Joo HJ, Kim ME, Seo YJ and Kim JI. (2008). Amelioration of plasma glucose and cholesterol levels in db/db mice by a misture of chinese herbs. Korean Journal of Medicinal Crop Science. 16:225-230.

원문보기 상세보기
Lee MK, Lee HS, Choi GP, Oh DH, Kim JD, Yu CY and Lee HY. (2003). Screening of biological acivities of the extracts from Rubus coreanus Miq. Korean Journal of Medicinal Crop Science. 11:5-12.

원문보기 상세보기
Matsubara M. (2004). Plasma adiponectin decrease in women with nonalcoholic fatty liver. Endocrine Journal. 51:587-593.

상세보기
Matsuda H, Kageura T, Inoue Y, Morikawa T and Yoshikawa M. (2000). Absolute stereostructures and synthesis of Saussureaamines A, B, C, D, and E, amino acid-sesquiterpene conjugates with gastroprotective effect, from the roots of Saussurea lappa. Tetrahedron. 56:7763-7777.

상세보기
Mitchell M, Armstrong DT, Robker RL and Norman RJ. (2005). Adipokines: implications for female fertility and obesity. Reproduction. 130:583-597.

상세보기
Mochizuki M and Hasegawa N. (2004). Effects of green tea catechin-induced lipolysis on cytosol glycerol content in differentiated 3T3-L1 cells. Phytotherapy Research. 18:945-946.

상세보기
Oh GS, Pae HO, Chung HT, Kwon JW, Lee JH, Kwon TO, Kwon SY, Chon BH and Yun YG. (2004). Dehydrocostus lactone enhances tumor necrosis factor-alpha-induced apoptosis of human leukemia HL-60 cells. Immunopharmacology and Immunotoxicology. 26:163-175.

상세보기
Pandey MM, Rastogi S and Rawat AK. (2007). Saussurea costus: botanical, chemical and pharmacological review of an ayurvedic medicinal plant. Journal of Ethnopharmacology. 110:379-390.

상세보기
Pi-Sunyer FX. (2002). The obesity epidemic: pathophysiology and consequences of obesity. Obesity Research. 10:97S-104S.

상세보기
Robinson A, Kumar TV, Sreedhar E, Naidu VG, Krishna SR, Babu KS, Srinivas PV and Rao JM. (2008). A new sesquiterpene lactone from the roots of Saussurea lappa: structure-anticancer activity study. Bioorganic & Medicinal Chemistry Letters. 18:4015-4017.

상세보기
Rosen ED. (2005). The transcriptional basis of adipocyte development. Prostaglandins, Leukotrienes, and Essential Fatty Acids. 73:31-34.

상세보기
Sartipy P and Loskutoff DJ. (2003). Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proceedings of the National Academy of Sciences. 100:7265-7270.

상세보기
Shoji N, Umeyama A, Saito N, Takemoto T, Kajiwara A and Ohizumi Y. (1986). Vasoactive substances from Saussurea lappa. Journal of Natural Products. 49:1112-1113.

상세보기
Taniguchi M, Kataoka T, Suzuki H, Uramoto M, Ando M, Arao K, Magae J, Nishimura T, Otake N, and Nagai K. (1995). Costunolide and dehydrocostus lactone as inhibitors of killing function of cytotoxic T lymphocytes. Bioscience, Biotechnology, and Biochemistry. 59:2064-2067.

상세보기
Tontonoz P, Hu E and Spiegelman BM. (1994). Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell. 79:1147-1156.

상세보기
Uygun A, Kadayifci A, Yesilova Z, Erdil A, Yaman H, Saka M, Deveci MS, Bagci S, Gulsen M, Karaeren N and Dagalp K. (2000). Serum leptin levels in patients with nonalcoholic steatohepatitis. American Journal of Gastroenterology. 95:3584- 3589.

상세보기
Wofford MR, Andrew ME, Brown A, King D, Pickett RA, Stevens J, Wyatt S and Jones DW. (1999). Obesity hypertension in the atherosclerosis risk in communities cohort: implications of obesity guidelines. Journal of Clinical Hypertension. 1:27-32.

상세보기
Yu HH, Lee JS, Lee KH, Kim KY and You YO. (2007). Saussurea lappa inhibits the growth, acid production, adhesion, and water-insoluble glucan synthesis of Streptococcus mutans. Journal of Ethnopharmacology. 111:413-417.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증