본 연구의 목적은 개 피부장벽 손상모델을 실험적으로 구현하기 위해 개의 정상피부, 제모 된 피부 그리고 아세톤 및 테잎 스트리립핑에 의한 손상된 피부에서의 표피내 칼슘이온의 분포를 관찰하였다. 피부손상에 따른 표피의 칼슘이온 변화도는 화학적 표시자(Calcium Green-1)가 포함된 gel blotting을 적용하여 피부장벽손상 효과를 시간에 따라 형광현미경으로 관찰하였다. 아세톤 손상 후 3분 및 1시간에 표피와 모낭의 칼슘이온의 차이를 나타내는 형광도차는 보다 밝게 관찰된 후 48시간 후에 소실되었다. 이와는 대조적으로, 테잎스트립핑손상 후 3분 및 1시간의 표피 칼슘이온의 형광도차는 아세톤 손상에서보다 더 밝게 보였다가 48시간 후에 소실되었다. 본 실험상의 방법을 통해 피부 손상 방법에 따른 표피 칼슘이온의 가시적 이미지를 관찰할 수 있었고, 표피내 농도차이를 확인할 수 있었다. 따라서 본 화학표시자를 이용한 염색법은 개 피부장벽 복구기전에 대한 칼슘이온의 역할을 규명하는데 유용할 것으로 판단되며, 추후 표피칼슘이온 농도의 정량분석법에 관한 연구가 필요할 것으로 사료된다.
본 연구의 목적은 개 피부장벽 손상모델을 실험적으로 구현하기 위해 개의 정상피부, 제모 된 피부 그리고 아세톤 및 테잎 스트리립핑에 의한 손상된 피부에서의 표피내 칼슘이온의 분포를 관찰하였다. 피부손상에 따른 표피의 칼슘이온 변화도는 화학적 표시자(Calcium Green-1)가 포함된 gel blotting을 적용하여 피부장벽손상 효과를 시간에 따라 형광현미경으로 관찰하였다. 아세톤 손상 후 3분 및 1시간에 표피와 모낭의 칼슘이온의 차이를 나타내는 형광도차는 보다 밝게 관찰된 후 48시간 후에 소실되었다. 이와는 대조적으로, 테잎스트립핑손상 후 3분 및 1시간의 표피 칼슘이온의 형광도차는 아세톤 손상에서보다 더 밝게 보였다가 48시간 후에 소실되었다. 본 실험상의 방법을 통해 피부 손상 방법에 따른 표피 칼슘이온의 가시적 이미지를 관찰할 수 있었고, 표피내 농도차이를 확인할 수 있었다. 따라서 본 화학표시자를 이용한 염색법은 개 피부장벽 복구기전에 대한 칼슘이온의 역할을 규명하는데 유용할 것으로 판단되며, 추후 표피칼슘이온 농도의 정량분석법에 관한 연구가 필요할 것으로 사료된다.
The purpose of this study is to establish experimental canine skin barrier disruption model, the study was designed to observe calcium ion in skin frozen tissue of canine skin and also the modulation of calcium ion distribution of normal skin with disrupted skin such as clipping, acetone, tape strip...
The purpose of this study is to establish experimental canine skin barrier disruption model, the study was designed to observe calcium ion in skin frozen tissue of canine skin and also the modulation of calcium ion distribution of normal skin with disrupted skin such as clipping, acetone, tape stripping damages according to time. To compare the changes of calcium ion gradient after damages, the distribution of calcium ion in the canine epidermis was visualized by blotting to gel containing chemical indicator (Calcium Green-1) with fluorescent microscope and the effects of skin barrier damages were examined according to time. Three mins and 1hr after acetone damage, the gradations of epidermis and hair follicle showed more radiant and disappeared after 48 hrs. On the contrary, 3mins and 1hr after tape stripping damage, the gradations showed more radiant than those of acetone damage, and these gradations were stabilized after 48 hrs. The method we presented here could show the visual image of the calcium ions in frozen tissue without further preparation, and it might be useful to investigate the role of calcium ion in the canine epidermal barrier recovery, however, it might be need further methodological improvement to get accurate quantitative information.
The purpose of this study is to establish experimental canine skin barrier disruption model, the study was designed to observe calcium ion in skin frozen tissue of canine skin and also the modulation of calcium ion distribution of normal skin with disrupted skin such as clipping, acetone, tape stripping damages according to time. To compare the changes of calcium ion gradient after damages, the distribution of calcium ion in the canine epidermis was visualized by blotting to gel containing chemical indicator (Calcium Green-1) with fluorescent microscope and the effects of skin barrier damages were examined according to time. Three mins and 1hr after acetone damage, the gradations of epidermis and hair follicle showed more radiant and disappeared after 48 hrs. On the contrary, 3mins and 1hr after tape stripping damage, the gradations showed more radiant than those of acetone damage, and these gradations were stabilized after 48 hrs. The method we presented here could show the visual image of the calcium ions in frozen tissue without further preparation, and it might be useful to investigate the role of calcium ion in the canine epidermal barrier recovery, however, it might be need further methodological improvement to get accurate quantitative information.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
However, there has been few study on epidermal ions in veterinary dermatology research. The purpose of this study is to establish experimental canine disrupted skin model for veterinary skin research. The study was designed to study calcium ion in skin frozen tissue of canine skin and alsothe modulation of calcium ion distribution of normal skin with disrupted skin such as clipping, acetone, tape stripping damages according to time.
제안 방법
The purpose of this study is to establish experimental canine disrupted skin model for veterinary skin research. The study was designed to study calcium ion in skin frozen tissue of canine skin and alsothe modulation of calcium ion distribution of normal skin with disrupted skin such as clipping, acetone, tape stripping damages according to time.
대상 데이터
Six clinically normal, 2-4-year-old, male Beagles without dermatological problems and abnormal blood health profiles were chosen for this study. The dogs were fed Natural Choice®Adult chicken and rice (Nutro co.
성능/효과
In conclusion, the observation of calcium ion would provide us information to understand epidermal homeostasis. The method we presented here could show the visual image of the calcium ions in frozen tissue without further preparation, and it might be useful to investigate the role of calcium ion in the canine epidermal barrier recovery, however, it might be need further methodological improvement to get accurate quantitative information.
후속연구
The method we presented here could show the visual image of the calcium ions in frozen tissue without further preparation, and it might be useful to investigate the role of calcium ion in the canine epidermal barrier recovery, however, it might be need further methodological improvement to get accurate quantitative information. Further study will be required to study magnesium ion and other ions in skin frozen tissue of canine skin as well as the modulation of these ions distribution in acetone and tape stripping damaged canine epidermis according to time.
In conclusion, the observation of calcium ion would provide us information to understand epidermal homeostasis. The method we presented here could show the visual image of the calcium ions in frozen tissue without further preparation, and it might be useful to investigate the role of calcium ion in the canine epidermal barrier recovery, however, it might be need further methodological improvement to get accurate quantitative information. Further study will be required to study magnesium ion and other ions in skin frozen tissue of canine skin as well as the modulation of these ions distribution in acetone and tape stripping damaged canine epidermis according to time.
참고문헌 (22)
Berardesca E, Pirot F, Singh M. Differences in stratum corneum ph gradient when comparing white caucasian and black african-american skin. Br J Dermatol 1998; 139: 855-857.
Bikle DD, Ratnam A, Mauro T, Harris J, Pillai S. Changes in calcium responsiveness and handling during keratinocyte differentiation. Potential role of the calcium receptor. J Clin Invest 1996; 97: 1085-1093.
Denda M, Fuziwara S, Inoue K. Influx of calcium and chloride ions into epidermal keratinocytes regulates exocytosis of epidermal lamellar bodies and skin permeability barrier homeostasis. J Invest Dermatol 2003; 121: 362-367.
Elias PM, Ahn SK, Denda M, Brown BE, Crumrine D, Kimutai LK, Komuves L, Lee SH, Feingold KR. Modulations in epidermal calcium regulate the expression of differentiation- specific markers. J Invest Dermatol 2002; 119: 1128- 1136.
Elias PM, Nau P, Hanley K, Cullander C, Crumrine D, Bench G, Sideras-Haddad E, Mauro T, Williams ML, Feingold KR. Formation of the epidermal calcium gradient coincides with key milestones of barrier ontogenesis in the rodent. J Invest Dermatol 1998; 110: 399-404.
Lee SH, Elias PM, Proksch E, Menon GK, Mao-Quiang M, Feingold KR. Calcium and potassium are important regulators of barrier homeostasis in murine epidermis. J Invest Dermatol 1992; 89: 530-538.
Mao-Qiang M, Mauro T, Bench G, Warren R, Elias PM, Feingold KR. Calcium and potassium inhibit barrier recovery after disruption, independent of the type of insult in hairless mice. Exp Dermatol 1997; 6: 36-40.
Mauro T, Bench G, Sidderas-Haddad E, Feingold K, Elias P, Cullander C. Acute barrier perturbation abolishes the $ca2^+$ and $k^+$ gradients in murine epidermis: Quantitative measurement using pixe. J Invest Dermatol 1998; 111: 1198-1201.
Menon GK, Elias PM, Lee SH, Feingold KR. Localization of calcium in murine epidermis following disruption and repair of the permeability barrier. Cell Tissue Res 1992; 270: 503-512.
Tanaka M, Zhen Y, Tagami H. Normal recovery of the stratum corneum barrier function following damage induced by tape stripping in patients with atopic dermatitis. Br J Dermatol 1997; 136: 966-967.
Yuspa S. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentration in vitro. J Cell Biol 1989; 109: 1207.
Zhai H, Poblete N, Maibach HI. Stripped skin model to predict irritation potential of topical agents in vivo in humans. Int J Dermatol 1998; 37: 386-389.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.