[논문]유근피 추출물의 피부개선효과

김영옥; 서용창; 이현용; 오명숙; 이상원; 김형돈

doi:10.7783/kjmcs.2011.19.6.508

유근피 추출물의 피부개선효과
Anti-Wrinkle Effect of Ulmus davidiana Extracts 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.19 no.6, 2011년, pp.508 - 513

김영옥 (국립원예특작과학원 인삼특작부) , 서용창 (강원대학교 의료.바이오신소재융복합연구센터) , 이현용 (강원대학교 의료.바이오신소재융복합연구센터) , 오명숙 (경희대학교 약학대학 한약학과) , 이상원 (국립원예특작과학원 인삼특작부) , 김형돈 (국립원예특작과학원 인삼특작부)

Abstract ▼ AI-Helper

The bark of the root and stem of Ulmus davidiana var. japonica has been used as a traditional Korean medicine to treat inflammatory disorders. This plant reportedly shows antioxidant, anticancer, and anti-inflammatory effects. In this study, we investigated the protective effects of Ulmus davidiana var. japonica ethanolic extract (UDE) on UVB irradiation-induced wrinkle in hairless mice. We evaluated for their free radical-scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the anti-elastase activities, and for their anti-matrix metalloproteinase-1 (MMP-1) activity in human skin fibroblast cells. In the wrinkle measurement and image analysis of skin replicas, the results showed that UDE significantly inhibited wrinkle formation caused by chronic UVB irradiation. These results suggest that UDE has anti-wrinkle activity.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Then, we hypothesized that UDE is a skin care cosmetics agent because of having antioxidative activity. Therefore, the main purpose of this study was to determine the biological activities such as antioxidant, anti-elastase, and anti-wrinkle activity of the UDE in vivo for further industrial applications.

가설 설정

Then, we hypothesized that UDE is a skin care cosmetics agent because of having antioxidative activity. Therefore, the main purpose of this study was to determine the biological activities such as antioxidant, anti-elastase, and anti-wrinkle activity of the UDE in vivo for further industrial applications.

제안 방법

In this study, topical application of UDE, which inhibits skin fibroblast-derived elastase, decreased UVB-induced wrinkle formation (evaluated by visible score and replica image) in mouse dorsal skin.
The active MMP-1 in culture supernatants were quantified by fluorescent assay, using the Fluorokine E Human Active MMP-1 Fluorescent Assay Kit (R&D Systems, Minneapolis, USA) in the cell culture supernatants was then determined using Quantikine ELISA kits (R&D Systems, Minneapolis, USA), according to the manufacturers protocol.

대상 데이터

UD (Ulmus davidiana) was collected from Kunsan in Korea (August 2004). A voucher specimen (HPR-207) was deposited at the herbarium of Herbal Crop Research Institute (Eumsung, Republic of Korea). Ulmus davidiana extract (UDE) was prepared as dried powder from the consecutive extractions with diluted ethanol, and provided by Kangwon National university.
A voucher specimen (HPR-207) was deposited at the herbarium of Herbal Crop Research Institute (Eumsung, Republic of Korea). Ulmus davidiana extract (UDE) was prepared as dried powder from the consecutive extractions with diluted ethanol, and provided by Kangwon National university. Specifically, UD (100 g) was extracted with 600 ㎖ of 55% ethanol at 50-53℃ for 24 h with stirring.

데이터처리

The wrinkle grading score and wrinkle area (%) were expressed as mean ± SD which were made in triplicate experiments. The paired t-test was used for comparisons between control group and UDE treated group. All analyses were performed using an SPSS 16 system (SPSS institute, Chicago, IL, USA).

이론/모형

, 2004). Assays of the free radical scavenging capacity were carried out by the DPPH method. The results being shown in Table 1.
Elastase inhibition activity was determined by the method of Tschesche and colleagues (Tschesche et al., 1992). In detail, 0.
Following 24 h of incubation, the culture supernatants were collected and centrifuged at 10,000 × g for 5 min to remove the particulate matter, and stored at −80℃ in fresh tubes. In supernatants, the protein concentration was determined using the Bradford method. The active MMP-1 in culture supernatants were quantified by fluorescent assay, using the Fluorokine E Human Active MMP-1 Fluorescent Assay Kit (R&D Systems, Minneapolis, USA) in the cell culture supernatants was then determined using Quantikine ELISA kits (R&D Systems, Minneapolis, USA), according to the manufacturers protocol.
(B) Visual scoring (scale ¼ 0-3) of wrinkles during UVB exposure of mice. The scoring was performed by the method of Bissett et al,. after UVB irradiation.

참고문헌 (28)

Bae JY, Lim SS, Kim SJ, Choi JS, Park J, Ju SM, Han SJ, Kang IJ and Kang YH. (2009). Bog blueberry anthocyanins alleviate photoaging in ultraviolet-B irradiation-induced human dermal fibroblasts. Molecular Nutrition & Food Research. 53:726-738.

상세보기
Berthod F, Germain L, Li H, Xu W, Damour O and Auger F. (2001). Collagen fibril network and elastic system remodeling in a reconstructed skin transplanted on nude mice. Matrix Biology. 20:463-473.

상세보기
Bissett DL, Chatterjee R and Hannon DP. (1990). Photoprotective effects of superoxide-scavenging antioxidants against ultraviolet radiation-induced chronic skin damage in the hairless mouse. Photodermatology, Photoimmunology & Photomedicine. 7:560-562.
Bolognia JL.(1993). Dermatologic and cosmetic concerns of the older woman. Clinics in Geriatric Medicine. 9:209-229.

상세보기
Eom SY, Kim YS, Lee CJ, Lee CH, Kim YD and Kim H. (2006). Cosmeceutical properties of polysaccharides from the root bark of Ulmus davidiana var. japonica. Journal of Cosmetic Science. 57:355-367

상세보기
Farkas B, Magyarlaki M, Csete B, Nemeth J, Rabloczky G, Bernath S, Literati Nagy P and Sumegi B. (2002). Reduction of acute photodamage in skin by topical application of a novel PARP inhibitor. Biochemical Pharmacology. 63:921-932.

상세보기
Godeau G and Hornebeck W. (1988). Morphometric analysis of the degradation of human skin elastic fibers by human leukocyte elastase (EC 3-4-21-37) and human skin fibroblast elastase (EC 3-4-24). Pathologie Biologie. 36:1133-1138.

상세보기
Ha JH, Kwon MC, Kim SS, Jeong MH, Hwang B and Lee HY. (2010). Enhancement of skin-whitening and UV-protective effects of Centella asiatica L. urban by ultrasonification process. Korean Journal of Medicinal Crop Science. 18:79-85.

원문보기 상세보기
Han SH, Woo NRY, Lee SD and Kang MH. (2006). Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean Journal of Medicinal Crop Science. 14:49-55.

원문보기 상세보기
Hastly KA, Jeffrey JJ, Hibbs MS and Welgus HG. (1987). The collagen substrate specificity of human neutrophil collagenase. Journal of Biological Chemistry. 262:10048-10052.
He G, Kutala VK, Kuppusamy P and Zweier JL. (2004). In vivo measurement and mapping of skin redox stress induced by ultraviolet light exposure. Free Radical Biology and Medicine. 36:665-672.

상세보기
Hong ND, Kim NJ and No YS. (1990). A study on efficacy of Ulmi cortex. Korean Journal of Pharmacognosy. 21:217-222.

원문보기 상세보기
Imokawa G, Takema Y, Yorimoto Y, Tsukahara K, Kawai M and Imayama S. (1995). Degree of ultraviolet-induced tortuosity of elastic fibers in rat skin is age dependent. Journal of Investigative Dermatology. 150:254-258.
Jeong SJ, Ha JH, Kim Y, Oh SH, Kim SS, Jeong MH and Lee HY. (2009). Effects of Rubus coreanus extracts on ultraviolet-A irradiated cultured human skin fibroblasts. Korean Journal of Medicinal Crop Science. 17:321-327.

원문보기 상세보기
Jin UH, Lee DY, Kim DS, Lee IS and Kim CH. (2006). Induction of mitochondria-mediated apoptosis by methanol fraction of Ulmus davidiana Planch (Ulmaceae) in U87 glioblastoma cells. Environmental Toxicology and Pharmacology. 22: 136-141.

상세보기
Learn DB and Moloney SJ. (1991). Numbers of murine dermal mast cells remain unchanged during chronic ultraviolet B irradiation. Photodermatology Photoimmunology & Photomedicine. 8:195-199.

상세보기
Lee IS , Wei C, Thuong PT, Song KS, Seong YH and Bae KH. (2006). Antioxidant constituents from the leaves of Cedrela sinensis A. Juss. Korean Journal of Medicinal Crop Science. 14:267-272.

원문보기 상세보기
Moloney SJ, Edmonds SH, Giddens LD and Learn DB. (1992). The hairless mouse model of photoaging: evaluation of the relationship between dermal elastin, collagen, skin thickness and wrinkles. Photochemistry and Photolobiology. 56:505-511.

상세보기
Murphy G and Docherty AJ. (1992). The matrix metalloproteinases and their inhibitors. American Journal of Respiratory Cell and Molecular Biology. 7:120-125

상세보기
Pillai S, Oresajo C and Hayward J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation a review. International Journal of Cosmetic Science. 27:17-34.

상세보기
Schwartz E, Feinberg E, Lebwohl M, Mariani TJ and Boyd CD. (1995). Ultraviolet radiation increases tropoelastin accumulation by a post-transcriptional mechanism in dermal fibroblasts. Journal of Investigative Dermatology. 105:65-69.

상세보기
Ta Takema Y, Nishijima A, Ohsu H, Fujimura T and Hattori M. (1997). Age-related discontinuous changes in the in vivo fluorescence of human facial skin. Journal of Dermatological Science. 15:55-58

상세보기
Takema, Y. and Imokawa G. (1998). The effects of UVA and UVB irradiation on the viscoelastic properties of hairless mouse skin in vivo. Dermatology. 196:397-400.

상세보기
Tschesche H, Bakowski B, Schettler A, Knauper V and Reinke H. (1991) Leukodiapedesis, release of PMN leukocyte proteinases and activation of PMNL procollagenase. Acta Biomedica Biochimica. 50:755-761
Tsukahara K, Takema Y, Moriwaki S, Tsuji N, Suzuki Y, Fujimura T and Imokawa G. (2011). Selective inhibition of skin fibroblast elastase elicits a concentration-dependent prevention of ultraviolet B-induced wrinkle formation. Journal of Investigative Dermatology. 117:671-677.
Welgus HG, Jeffrey JJ and Eisen AZ. (1981). The collagen substrate specificity of human skin fibroblast collagenase. The Journal of Biological Chemistry. 256:9511-9515.
Wiedow O, Schroder JM, Gregory H, Young JA and Christophers E. (1990). Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. The Journal of Biological Chemistry. 265:14791-14795.
Zimbler MS, Kokoska MS and Thomas JR. (2001). Anatomy and pathophysiology of facial aging. Facial Plastic Surgery Clinics of North America. 9:179-187.

상세보기

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증