[논문]Snowdrop lectin (Galanthus nivalis agglutinin: GNA) 유전자 도입에 의한 벼멸구 저항성 형질전환 벼 개발

이수인; 윤인선; 김진아; 홍준기; 박범석; 이연희

doi:10.5352/jls.2012.22.12.1614

Snowdrop lectin (Galanthus nivalis agglutinin: GNA) 유전자 도입에 의한 벼멸구 저항성 형질전환 벼 개발
Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin; GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stål) 원문보기

생명과학회지 = Journal of life science, v.22 no.12 = no.152, 2012년, pp.1614 - 1620

이수인 (국립농업과학원 농업생명자원부) , 윤인선 (국립농업과학원 농업생명자원부) , 김진아 (국립농업과학원 농업생명자원부) , 홍준기 (국립농업과학원 농업생명자원부) , 박범석 (국립농업과학원 농업생명자원부) , 이연희 (국립농업과학원 농업생명자원부)

초록
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유전자총(Particle bombardment) 방법에 의해 snowdrop lectin (Galanthus nivalis agglutinin; GNA) 유전자가 도입된 해충저항성 형질전환 벼를 개발하였다. GNA 유전자가 도입된 많은 형질전환 식물체가 재분화되었으며, 형질전환 벼의 GNA 유전자의 integration, expression 및 inheritance는 Southern 및 western analysis분석 방법에 의해 증명되었다. 벼 genome 내 도입 유전자 수는 one 혹은 five copies이었다. 형질전환 $R_1$과 $R_2$ 식물체의 GNA 단백질 발현 분석 결과, 함량은 총 단백질 중 0.01%부터 2.0%까지 포함하고 있었다. 형질전환 식물체 중 GNA 과발현 형질전환 벼를 이용한 생물 검정 결과, 대조구와 비교하여 벼의 주요 해충인 벼멸구(Nilaparvata lugens St${\aa}$l)에 저항성을 나타내었다. 위의 실험 결과를 통해, 해충저항성 유전자인 GNA가 도입된 형질전환 벼는 해충의 증식을 억제하는데 이용될 수 있을 것이다.

Abstract ▼ AI-Helper

Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens St${\aa}$l) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated. The integration, expression, and inheritance of this gene in the $R_1$ and $R_2$ generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the $R_1$ and $R_2$ transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the $R_2$ transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA into rice plants therefore can help to control insect pests.

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성능/효과

5; right). After 4 weeks, both egg production and the number of nymphs produced by the adults feeding on transgenic rice plants were reduced by approximately 6-fold compared with the control. The results of this bioassay provide further evidence that GNA can reduce insect attacks on rice, and that GNA has insecticidal activity against BPH.
After 4 weeks, both egg production and the number of nymphs produced by the adults feeding on transgenic rice plants were reduced by approximately 6-fold compared with the control. The results of this bioassay provide further evidence that GNA can reduce insect attacks on rice, and that GNA has insecticidal activity against BPH. We hope to conduct further large-scale experiments under field conditions.

후속연구

The results of this bioassay provide further evidence that GNA can reduce insect attacks on rice, and that GNA has insecticidal activity against BPH. We hope to conduct further large-scale experiments under field conditions. As tissue-specific expression of a single GNA gene in transgenic plants induced insect resistance, we are currently investigating the use of other tissue-specific promoter (RSs1, which is a phloem-specific promoter) to further increase resistance to insect pests.

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