목 적 : 본 연구에서는 생강나무 메탄올 추출물이 암전이 억제에 미치는 영향을 조사하고자 하였다. 방 법 : 생강나무 추출물의 암전이 억제능을 확인하기 위해서 B16F10 흑색종 세포를 이용하여 금속단백분해효소의 활성 및 발현을 측정하였으며, 암세포의 이동능이나 침윤능도 조사하였다. 폐전이 동물모델에서 생강나무 추출물이 미치는 영향을 조사하여 활성을 최종적으로 확인하였다. 결 과 : 1. 생강나무 추출물은 B16F10 흑색종 세포에서 뚜렷한 금속단백분해효소의 효소활성 및 발현 억제효과를 보였으며 이는 NF-${\kappa}B$의 활성 억제에서 기인한 것임을 확인하였다. 2. 흑색종 세포의 이동이나 침윤 역시 생강나무 추출물 투여에 의해 현저히 감소하였다. 3. 폐전이 동물 모델에서도 생강나무 추출물에 의해 폐로 전이되 집락의 수가 감소하였다. 결 론 : 이상의 결과로 생강나무 추출물은 뛰어난 암전이 억제효과가 있는 것을 확인할 수 있었으며, 전이성 암치료에 있어서 유용하게 사용될 수 있을 것으로 사료된다.
목 적 : 본 연구에서는 생강나무 메탄올 추출물이 암전이 억제에 미치는 영향을 조사하고자 하였다. 방 법 : 생강나무 추출물의 암전이 억제능을 확인하기 위해서 B16F10 흑색종 세포를 이용하여 금속단백분해효소의 활성 및 발현을 측정하였으며, 암세포의 이동능이나 침윤능도 조사하였다. 폐전이 동물모델에서 생강나무 추출물이 미치는 영향을 조사하여 활성을 최종적으로 확인하였다. 결 과 : 1. 생강나무 추출물은 B16F10 흑색종 세포에서 뚜렷한 금속단백분해효소의 효소활성 및 발현 억제효과를 보였으며 이는 NF-${\kappa}B$의 활성 억제에서 기인한 것임을 확인하였다. 2. 흑색종 세포의 이동이나 침윤 역시 생강나무 추출물 투여에 의해 현저히 감소하였다. 3. 폐전이 동물 모델에서도 생강나무 추출물에 의해 폐로 전이되 집락의 수가 감소하였다. 결 론 : 이상의 결과로 생강나무 추출물은 뛰어난 암전이 억제효과가 있는 것을 확인할 수 있었으며, 전이성 암치료에 있어서 유용하게 사용될 수 있을 것으로 사료된다.
Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions ...
Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO. Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-${\kappa}B$ in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo. Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.
Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO. Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-${\kappa}B$ in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo. Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.
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문제 정의
obtusiloba are not reported. Therefore, this study was undertaken to validate the anti-metastatic activity of L. obtusiloba in the highly metastatic murine melanoma B16F10 cell line. To test the possibility whether L.
제안 방법
It is well known that the B16F10 cells have an strong invading properties through Matrigel. In this study, we investigate the inhibitory effect of MLO on cell invasion using BD BioCoat Matrigel chamber. Treatment MLO for 22 h demonstrated that excellent suppression on the melanoma cell invasion in a dose dependent manner (Fig.
1% DMSO as the solvent control. The wound closure was monitored and photographed at 0 and 24 h using inverted microscope and camera (Nikon, Japan).
obtusiloba in the highly metastatic murine melanoma B16F10 cell line. To test the possibility whether L. obtusiloba can alter the cancer metastasis process, we checked MMP-2 and MMP-9 activities and expressions as well as migration and invastion in B16F10 cells. We also determined its inhibitory effects on the lung metastasis in vivo.
대상 데이터
C57BL/6 male mice, with an age of 6-week-old, were injected with 5×105 B16F10 melanoma cells (0.2 ml/mouse) in FBS free DMEM subcutaneously.
The plant materials were purchased from Hainyakupsa (Jeonbuk, South Korea) in June 2010. A voucher specimen (WME068) has been deposited at the Department of Oriental Pharmacy, College of Pharmacy, Woosuk University.
이론/모형
Cell viability was evaluated by MTT colorimetric assay as described in the method. The results are expressed as means ± S.
Thus, inhibition of cell invasion and migration also could be a useful anti-metastatic strategy. Here in this work, we evaluated the cell invasion using Matrigel invasion assay. The results showed that MLO attenuated invasion of B16F10 cells significantly in a dose dependent manner.
The protein extracts were prepared; samples were analyzed for MMP-9 expression by western blotting as described in the method. Determined activities of MMP-9 were subsequently quantified by densitometric analysis with that of non-treated control being 100% as shown just below the gel data.
성능/효과
Based on these results, further anti-metastatic studies were undertaken. Zymogram results from this study demonstrated that MLO inhibited enzymatic activities in both of MMP-2 and MMP-9 in a dose dependent manner. Furthermore, MLO also down-regulated protein levels of MMP-2 and MMP-9.
후속연구
1, the clear zone by bacterial collagenase activity was decreased following addition of MLO, compared to control group. Thus it was strongly believed that MLO may be one of the effective MMP inhibitor and based on these results, further studies were undertaken.
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