[논문]RAPD 마커에 의한 한국, 중국, 일본 참가리비의 유전적 다양성과 집단 구조

남명모; 이주; 문태석; 허만규

doi:10.5352/jls.2012.22.4.466

RAPD 마커에 의한 한국, 중국, 일본 참가리비의 유전적 다양성과 집단 구조
Genetic Diversity and Population Structure of the Scallop Patinopecten yessoensis in Korea, China, and Japan by Random Amplified Polymorphic DNA Markers 원문보기

생명과학회지 = Journal of life science, v.22 no.4 = no.144, 2012년, pp.466 - 471

남명모 (국립수산과학원) , 이주 (국립수산과학원) , 문태석 (국립수산과학원) , 허만규 (동의대학교 분자생물학과)

초록
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참가리비($Patinopecten$ $yessoensis$) 60개체를 채집하여 유전적 다양성과 집단구조를 조사하였다. 임의 유전 다형성 DNA (RAPD)로 109개 유전자형과 79개 다형성 좌위(72.8%)를 발견하였다. 전체 유전적 다양도($H_T$)와 집단내 변이($H_S$)는 각각 0.254와 0.178였다. 유전자 좌위에 근거하여 집단 간 분화 정도($G_{ST}$)는 0.299였다. 이는 전체변이의 약 70.1%가 집단 내에 존재하고 있음을 시사한다. 참가리비 세 나라 집단에서 한 집단에 국한되는 대립유전자 좌위와 한 개체에만 발현된 밴드가 발견되었다. RAPD 마커는 한국, 중국, 일본에 분포하는 참가리비를 구분하는데 매우 효과적이었다. 또한 참가리비의 집단 내, 집단 간 유전적 다양성에 대한 통찰은 동물 유전자원의 수진 전략과 양식에 유익할 것으로 사료된다.

Abstract ▼ AI-Helper

Sixty individuals of the scallop $Patinopecten$ $yessoensis$ (Genus Pecten) were sampled to examine the genetic diversity and population structure of this species. Random amplified polymorphic DNA (RAPD) identified 109 genotypes and produced 79 polymorphic loci (72.8%). Total genetic diversity values ($H_T$) and interlocus variation in the within-population genetic diversity ($H_S$) were 0.254 and 0.178, respectively. On a per-locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) was 0.299. This indicated that about 70.1% of the total variation was within populations. The unique loci and bands of $P.$ $yessoensis$ were shown in only one population among the three countries. RAPD markers were very effective in classifying the natural population levels of $P.$ $yessoensis$ in Korea, China, and Japan. In addition, insights into the relative gene diversity among and within populations of $P.$ $yessoensis$ would be useful in breeding and for the development of strategies for animal genetic resources.

주제어

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문제 정의

This study was carried out to examine three populations of P. yessoensis in order to evaluate genetic diversity and population structure in this species.

가설 설정

yessoensis amplified with OPA-12 primer. M: Molecular weight of standard.

제안 방법

In this study, three populations of P. yessoensis from Korea, China and japan were analyzed for RAPD (random amplified polymorphic DNA) markers. RAPD assay has been useful in determining genetic relationships among closely related species [13].
It is expected the sperms are disperse only a short distance from the parents, producing a patchy distribution. The Mantel test supported this by showing correlation between genetic distance and spatial distribution. The data are consistent with the dispersal of spat being an important factor in the population structure of this species.
The Mantel test was examined the correlation between the matrix of genetic distance and spatial distance within a site.
The Mantel test was used to test for correlations between the matrix of genetic diversity and spatial distance. Genetic diversity correlated with spatial distance in P.

이론/모형

A genetic relationship was constructed by the neighbor joining (NJ) method [14] using the NEIGHBOR program in MEGA5 [16].
Clustering of accessions was performed based on the matr ix of calculated distances using the NJ algorithm (Fig. 3). In dendrogram, all populations were well separated from each other.

성능/효과

From the sixty decamer primers used for a preliminary RAPD analysis, eleven primers of them produced good amplification products both in quality and variability (Table 2) For the sixty samples, these eleven primers produced 109 scorable bands, 79 of which were polymorphic (85.5%). Fig.

참고문헌 (19)

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