[논문]사료내 벤조산 첨가가 이유돼지의 장내 미생물 균총 및 면역체계에 미치는 영향

오희경; 최영환; 김영해; 김유용

doi:10.5352/jls.2012.22.10.1307

사료내 벤조산 첨가가 이유돼지의 장내 미생물 균총 및 면역체계에 미치는 영향
Effect of Dietary Benzoic Acid on Beneficial Microflora and Immune Response in the Intestine of Weaning Pigs 원문보기

생명과학회지 = Journal of life science, v.22 no.10 = no.150, 2012년, pp.1307 - 1315

오희경 (장안대학교 식품영양과) , 최영환 (서울대학교 동물생명과학과) , 김영해 (연변대학교 동물과학과) , 김유용 (서울대학교 동물생명과학과)

초록
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본 연구에서는 이유자돈 사료에 벤조산 첨가가 위장 내 소화물과 방광 내 뇨의 pH, 장내 유익 미생물 균총수 및 장 조직 내 염증성 싸이토카인 유전자 발현양에 미치는 영향을 조사하였다. 자돈기 5주 동안 이유자돈 사료 내 벤조산 0.3%과 0.5% 첨가는 자돈 위 내 pH는 감소시키지는 않았으나, 방광 내 뇨의 pH 수치는 유의적으로 감소하는 것으로 나타났다. Lactobacillus casei 수는 맹장부위에서 벤조산 0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났다. Lactobacillus plantarum 수는 맹장부위에서 벤조산 0.5% 첨가구에서 가장 높게 나타났다. 또한, 직장부위에서 L.plantarum 수는 대조구를 제외하고 모든 처리구에서 유의적으로 높게 나타났다. Bacillus subtillis 수는 맹장부위에서 벤조산0.5% 첨가구와 대조구에서 유의적으로 가장 높게 나타났으며, 직장부위에서는 벤조산0.3%와 0.5% 첨가구에서 가장 높게 나타났다. IL-$1{\beta}$ mRNA 유전자 발현 수준은 소장 상단에서는 벤조산 0.5%와 항생제 처리구에서, 소장중부에서는 벤조산 0.3%와 0.5% 처리구에서, 말단부위에서는 벤조산 0.3% 처리구에서 대조구보다 유의적으로 낮게 나타났다. IL-6 mRNA 유전자 발현 수준은 소장중부에서는 벤조산 0.5% 처리구에서 대조구보다 유의적으로 낮게 나타났다. TNF-${\alpha}$ mRNA 유전자 발현 수준은 소장 중단에서 벤조산 0.5%와 항생제 처리구에서 대조구 보다 낮게 나타났다. 본 실험을 통하여, 자돈 사료 내 benzoic acid 0.5% 첨가는 뇨의 pH 감소와 장내 유익 미생물 균총 수 조절 및 소장 내 면역체계 반응에 긍정적인 영향을 미치므로 자돈 건강을 향상 할 수 있으리라 사료된다.

Abstract ▼ AI-Helper

We evaluated the effect of dietary supplements with benzoic acid on intestinal beneficial bacteria concentration and immune response of weaning pigs. Supplementation with benzoic acid at 0.5% or control diet for 35 days resulted in a higher Lactobacillus casei concentration in the cecum. Supplementation with benzoic acid at 0.5% increased concentration of L. plantarum in the cecum. Pigs with the control diet and 0.5% benzoic acid had significantly increased concentration of B. subtillis in the cecum compared to the antibiotic group, while the concentration of B. subtillis in the rectum increased in pigs given 0.3 and 0.5% benzoic acid (p<0.05). Compared with the control group, the level of interleukin-$1{\beta}$ mRNA showed a significant decrease in the proximal small intestine in pigs fed diets supplemented with benzoic acid at 0.5% or antibiotic. Feeding 0.5% benzoic acid resulted in a marked reduction in the expression of IL-6 mRNA in the middle small intestine (p<0.05). Supplementation with benzoic acid at 0.5% or antibiotic resulted in a lower level of tumor necrosis factor-mRNA in the middle intestine. Up to 0.5% benzoic acid may be included in weaning diets for improvement of intestinal beneficial bacteria, thus modulating genes of pro-inflammatory cytokines in the gastrointestinal tract.

주제어

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문제 정의

However, little research has been done about effect of benzoic acid on inhabitation of beneficial bacteria through inhibiting proliferation of pathogenic bacteria in the GIT by using benzonic acid in feed. Therefore, this study was to investigate the effect of benzoic acid on populations of specific beneficial bacteria, thus, improve the immune response through modulation of gene expression of proinflammatory cytokines in the GIT.

제안 방법

998, respectively. In this study, examination of specific beneficial bacteria was designed using absolute quantification of 16S rRNA and real-time PCR. Quantification can be performed relative to an external standard curve, using serial dilutions of an external standard for generation of a standard curve of Ct (threshold cycle) against the initial target copy numbers.
47). Using a randomized complete block design that was replicated three times, the animals were allocated for 35 days into four experimental treatment groups based on their initial body weight and gender.

대상 데이터

A total of 32 crossbred (Landrace x Yorkshire x Duroc) pigs were used. The pigs were weaned at 24 days of age with an average initial body weight of 6.

데이터처리

Statistical analyses of data on pH, microbial populations, and proinflammatory cytokine mRNA expression levels in intestine were performed with ANOVAs using the General Linear Model procedure of the SAS (SAS Inst., Inc., Cary, NC) software. Comparison between treatments was subsequently conducted using the least-squares or Duncan methods.

이론/모형

, Cary, NC) software. Comparison between treatments was subsequently conducted using the least-squares or Duncan methods. The variance is expressed as the standard error of the mean (SEM) and a P value of less than 0.
The purity of PCR product was verified by generating melting curve at the end of the thermal cycling. Relative level of mRNA was analyzed by the comparative C_t method. All C_t values of each sample were normalized to that of β-actin, being used for statistical analysis.
The relative amounts of target genes were calculated after normalization to a reference (β-actin) with the arithmetic formula of Kenneth and Thomas.

성능/효과

In conclusion, inclusion levels of benzoic acid up to 0.5% can be advantageous as a result of encouraging proliferation of specific beneficial bacteria, thus having a positive impact on gene expression of inflammatory cytokines in the intestine.
5% showed a reduced level of inflammatory cytokine gene expression in the different segments of the small intestine, compared with animals fed the control diet. This finding provides evidence that inclusion of 0.5% benzoic acid creates a hospitable environment for beneficial bacteria in GIT, which ultimately leads to an increase in their population size. This increase may be responsible for preventing production of inflammatory mediators, which could have a negative effect on the integrity of the intestine.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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