연안 암반지역에서 해조류 군락의 소실 즉 백화 혹은 갯녹음현상은 산호조류와 관련성이 있다. 대표적인 무절산호조의 생물학적 특성을 파악하기 위하여 18S rDNA 유전자를 분석한 결과 혹돌잎(Lithophyllum) 속에 속하는 것을 확인하였고 그 형태적 특성으로 보아 L. yessoense 종인 것으로 유추된다. Triphenyl tetrazolium chloride로서 활력을 측정한 결과 12월에서 2월 사이가 가장 높았으며, 조직 활력을 유지하기 위하여는 $16^{\circ}C$, 16:8 시간 명암 광주기, 30 ${\mu}E/m^2/s$ 광도에서 5일간 최적상태를 보였다. 지방산 조성에서는 EPA가 가장 많은 고도불포화지방산으로서 9.7%를 차지하고 있다. 주사형전자현미경에 의한 표면구조를 보면 평균 3.6 ${\mu}m$ 직경의 둥근 함몰 분화구 모양을 이루며 그 위에 1.0 내지 3.7 ${\mu}m$의 비정형 다각형 구조물들이 덮여져 있다. 이 같은 조성과 구조를 바탕으로 한 생체모방 산호조는 해조류 등에 대한 환경친화적 방오소재로서 활용되어질 수도 있을 것이다.
연안 암반지역에서 해조류 군락의 소실 즉 백화 혹은 갯녹음현상은 산호조류와 관련성이 있다. 대표적인 무절산호조의 생물학적 특성을 파악하기 위하여 18S rDNA 유전자를 분석한 결과 혹돌잎(Lithophyllum) 속에 속하는 것을 확인하였고 그 형태적 특성으로 보아 L. yessoense 종인 것으로 유추된다. Triphenyl tetrazolium chloride로서 활력을 측정한 결과 12월에서 2월 사이가 가장 높았으며, 조직 활력을 유지하기 위하여는 $16^{\circ}C$, 16:8 시간 명암 광주기, 30 ${\mu}E/m^2/s$ 광도에서 5일간 최적상태를 보였다. 지방산 조성에서는 EPA가 가장 많은 고도불포화지방산으로서 9.7%를 차지하고 있다. 주사형전자현미경에 의한 표면구조를 보면 평균 3.6 ${\mu}m$ 직경의 둥근 함몰 분화구 모양을 이루며 그 위에 1.0 내지 3.7 ${\mu}m$의 비정형 다각형 구조물들이 덮여져 있다. 이 같은 조성과 구조를 바탕으로 한 생체모방 산호조는 해조류 등에 대한 환경친화적 방오소재로서 활용되어질 수도 있을 것이다.
The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the ...
The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the 18S rDNA gene was sequenced to identify the genus Lithophyllum. According to its morphological and distributional characteristics, it was deduced to be L. yessoense. Viability was measured using triphenyl tetrazolium chloride and showed high viability from December to February. Culture conditions of $16^{\circ}C$, a 16 hr light, 8 hr dark cycle, and 30 ${\mu}E/m^2/s$ light intensity were optimal for maintaining the viability of the alga for up to five days. Included in the fatty acids was 9.7% ${\omega}$-3 eicosapentaenoic acid. An electron microscopy scan of the surface structure revealed round craters about 3.6 ${\mu}m$ in diameter, which were covered with rough, irregular, and angular polygon-shaped structures about 1.0 to 3.7 ${\mu}m$ in size. Based on the composition and structure found in our study, biomimetic coralline alga might become an environmentally friendly antifouling material against the attachment of soft foulants.
The disappearance of seaweed flora in some rocky areas, which is known as algal whitening, barren ground, coralline flats, or deforested areas, is associated with some species of coralline algae. To determine the biological characteristics of a representative species of crustose coralline alga, the 18S rDNA gene was sequenced to identify the genus Lithophyllum. According to its morphological and distributional characteristics, it was deduced to be L. yessoense. Viability was measured using triphenyl tetrazolium chloride and showed high viability from December to February. Culture conditions of $16^{\circ}C$, a 16 hr light, 8 hr dark cycle, and 30 ${\mu}E/m^2/s$ light intensity were optimal for maintaining the viability of the alga for up to five days. Included in the fatty acids was 9.7% ${\omega}$-3 eicosapentaenoic acid. An electron microscopy scan of the surface structure revealed round craters about 3.6 ${\mu}m$ in diameter, which were covered with rough, irregular, and angular polygon-shaped structures about 1.0 to 3.7 ${\mu}m$ in size. Based on the composition and structure found in our study, biomimetic coralline alga might become an environmentally friendly antifouling material against the attachment of soft foulants.
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이론/모형
1. Phylogenetic dendrograms of the crustose coralline alga based on 18S rDNA sequence, and constructed using the neighbor-joining method. Numbers at nodes indicate the level of bootstrap support (1,000 replicates).
The sequences were edited and manipulated using MEGA3 [12]. Phylogenetic trees were inferred using the neighbor-joining algorithm [19] in MEGA3 with bootstrap analysis of 1,000 bootstrap replications.
The amplification products were sequenced using the same primers (SolGent, Daejeon, Korea). The sequences were edited and manipulated using MEGA3 [12]. Phylogenetic trees were inferred using the neighbor-joining algorithm [19] in MEGA3 with bootstrap analysis of 1,000 bootstrap replications.
To ascertain the species identification, we determined partial 18S rDNA gene sequence (NCBI submission # 1609660). They were then aligned and analyzed using the neighbor-joining method to construct a dendrogram. The 1,391-bp 18S rDNA sequence from base 274 to 1,664 was compared to the sequences of 13 species of coralline algae obtained from the NCBI database to infer the phylogenetic relationship (Fig.
성능/효과
Sloughing in this case is probably a means of eliminating old reproductive structures and grazer-damaged surface cells, and reducing the likelihood of surface penetration by burrowing organisms. SEM-based energy-dispersive X-ray spectroscopy showed that the relative elemental composition was 50% carbon, 39% oxygen, 3% sodium, 2% chlorine, and 6% calcium by atomic percentage, or 37% carbon, 38% oxygen, 5% sodium, 5% chlorine, and 15% calcium by weight. The major mineral content was calcium carbonate.
The alga used in this study has typically no margins ridged where crusts meet, and is not much thick, dull chalky and yellowish. Thus, the alga was identified to be belonging to the crustose coralline Lithophyllum genus using the 18S rDNA sequence, but not able to confirm the species level. By the morphological shape, the crustaceous thalli spread irregularly like a pinky patch, non-verrucose on surface.
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