[논문]Effect of Cholera Toxin Administered Supraspinally or Spinally on the Blood Glucose Level in Pain and D-Glucose Fed Animal Models

Sim, Yun-Beom; Park, Soo-Hyun; Kang, Yu-Jung; Kim, Sung-Su; Kim, Chea-Ha; Kim, Su-Jin; Jung, Jun-Sub; Ryu, Ohk-Hyun; Choi, Moon-Gi; Choi, Seong-Soo; Suh, Hong-Won

doi:10.4196/kjpp.2013.17.2.163

Effect of Cholera Toxin Administered Supraspinally or Spinally on the Blood Glucose Level in Pain and D-Glucose Fed Animal Models 원문보기

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, v.17 no.2, 2013년, pp.163 - 167

Sim, Yun-Beom (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Park, Soo-Hyun (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Kang, Yu-Jung (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Kim, Sung-Su (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Kim, Chea-Ha (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Kim, Su-Jin (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Jung, Jun-Sub (Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University) , Ryu, Ohk-Hyun (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University) , Choi, Moon-Gi (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University) , Choi, Seong-Soo (Department of Anesthesiology and Pain Medicine, Asan Medical Center) , Suh, Hong-Won

Abstract ▼ AI-Helper

In the present study, the effect of intrathecal (i.t.) or intracerebroventricular (i.c.v.) administration with cholera toxin (CTX) on the blood glucose level was examined in ICR mice. The i.t. treatment with CTX alone for 24 h dose-dependently increased the blood glucose level. However, i.c.v. treatment with CTX for 24 h did not affect the blood glucose level. When mice were orally fed with D-glucose (2 g/kg), the blood glucose level reached to a maximum level at 30 min and almost returned to the control level at 120 min after D-glucose feeding. I.c.v. pretreatment with CTX increased the blood glucose level in a potentiative manner, whereas i.t. pretreatment with CTX increased the blood glucose level in an additive manner in a D-glucose fed group. In addition, the blood glucose level was increased in formalin-induced pain animal model. I.c.v. pretreatment with CTX enhanced the blood glucose level in a potentiative manner in formalin-induced pain animal model. On the other hand, i.t. pretreatment with CTX increased the blood glucose level in an additive manner in formalin-induced pain animal model. Our results suggest that CTX administered supraspinally or spinally differentially modulates the regulation of the blood glucose level in D-glucose fed model as well as in formalin-induced pain model.

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v. (B) for 24 h. The blood glucose level was measured after 24 h after CTX pretreatment. The vertical bars indicate the standard error of mean (**p＜0.
05 to 0.5μg/5μl) for 24 h. The blood glucose level was measured at 30, 60, and 120 min after either saline or D-glucose (2 g/kg) was fed orally. The data were analyzed by area under the curve (C, D).

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Statistical analysis was carried out by student t test GraphPad Prism Version 4.0 for Windows (GraphPad Software, San Diego, CA, USA). The area under the curve (AUC) was analyzed by one-way ANOVA.
0 for Windows (GraphPad Software, San Diego, CA, USA). The area under the curve (AUC) was analyzed by one-way ANOVA. p values less than 0.

후속연구

In addition, we found recently that substance P and other types of pro-inflammatory cytokines such as TNF-α, IL-1β and IFN-γ administered spinally causes an elevation of the blood glucose level [9,14]. Studies on the detailed role of the spinal cord in the regulation of the blood glucose level should be further investigated in the future study.

참고문헌 (16)

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