[논문]Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities

Han, JeungHi; Kim, Mi-Ryung; Park, Yooheon; Hong, Yang Hee; Suh, Hyung Joo

doi:10.5851/kosfa.2013.33.3.356

Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.33 no.3, 2013년, pp.356 - 362

Han, JeungHi (Department of Food and Nutrition, Korea University) , Kim, Mi-Ryung (Department of Home Economics, Korea University) , Park, Yooheon (Department of Food and Nutrition, Korea University) , Hong, Yang Hee (Department of Food and Nutrition, Korea University) , Suh, Hyung Joo (Department of Food and Nutrition, Korea University)

Abstract ▼ AI-Helper

We investigated the skin permeability and various biological activities of porcine homogenate of placenta (HP) with the highest protein contents (452.89 ${\mu}g/mg$). The content of protein in subcritical extract of HP (SPE) was decreased from the initial content of 452.9 ${\mu}g/mg$ to 262.7 ${\mu}g/mg$ at 3 h subcritical extract. The contents of amino type nitrogen (A-N) were sharply increased from 35.1 ${\mu}g/mg$ of initial content to 305.9 ${\mu}g/mg$ at 3 h subcritical extract. The HP showed a noticeable activity in terms of antioxidant capacity for ferric reducing antioxidant power (FRAP) assay and especially for 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. HP, SPE-0.5, SPE-2 and SPE-3 showed inhibitory effect on elastase activities with an $IC_{50}$ of 46.1, 42.9, 31.6 and 34.7 ${\mu}g/mL$, respectively. SPEs showed more significantly inhibitory effect than HP (p<0.05). The skin permeability of the SPEs was higher than that of the HP. SPE-3 showed highest skin permeation and the permeability was significantly higher than that of HP. SPE-2 also showed significantly higher permeation than HP after 4 h. As expected, increase of extraction time significantly increased skin permeability in the subcritical extract of HP (SPE). From these results, in terms of cost and source availability, porcine placenta extracted with subcritical extraction has advantages over untreated PE and have potential as a cosmetic ingredient.

주제어

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가설 설정

For the use of porcine PE as a cosmeceutical, dermal absorption is an important factor for cosmetic ingredients. In this study, we investigated the skin permeability and various biological activities of porcine PE as cosmeceutical ingredients.

대상 데이터

(2008), with certain modifications. Male Sprague-Dawley (SD) rats, weighing 250 to 300 g (Nara Bio Animal Center, Korea), were used for the study. The hair was removed from rats with an electric clipper and an electric razor 1 day before the study.

데이터처리

The significance of the differences was analyzed by using one-way analysis of variance (ANOVA) with Duncan’s multiple range tests.
Protein and A-N contents of porcine PE. Values were analyzed with a repeated measurement ANOVA. Each bar represented mean±SD.
Values were analyzed with a repeated measurement independent t-test (*: significantly different at p<0.05; **: significantly different at p<0.01; ***: significantly different at p<0.001).

이론/모형

4. Skin permeability of PE using Franz cell model. Values were analyzed with a repeated measurement independent t-test (*: significantly different at p<0.
The assay was carried out based on the method of James (Kraunsoe et al., 1996). One µg porcine pancreatic elastase type IV (PPE) diluted in 0.
Protein content was assayed using the bicinchoninic acid (BCA) method, according to the manufacturer’s instructions (Pierce Chemicals Ltd, USA) using bovine serum albumin as the standard. The contents of amino type nitrogen (A-N) were determined by the methods of TNBS. Modified methods of Adler-Nissen (1979) were used to determine α-amino acid content.
The protein content of the transports was determined by using the bicinchoninic acid (BCA) method, according to the manufacturer’s instructions (Pierce Chemicals Ltd, USA).
Intestinal transport of the sample was expressed as mg of protein/g tissue dry weight. The protein contents of the transports were determined according to the BCA method.

성능/효과

The ABTS radical scavenging activity was determined as described by Wang and Xiong (2005), with slight modification. The antioxidant activities of the test samples were expressed as IC₅₀, i.e., the amount of tested extract required for a 50% decrease in the absorbance of the ABTS radicals. The ferric reducing antioxidant power (FRAP) method was performed as described by Benzie and Strain (1996).

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