In this study, we selected some material to have potential bioactivity from natural plants, confirmed as basic data for industrializing and tried to develope the food materials using them. DPPH, ABTS, antioxidant protection factor, TBARs and antimicrobial activity of extracts from Rosa multiflora Th...
In this study, we selected some material to have potential bioactivity from natural plants, confirmed as basic data for industrializing and tried to develope the food materials using them. DPPH, ABTS, antioxidant protection factor, TBARs and antimicrobial activity of extracts from Rosa multiflora Thunberg fruits were determined. The total phenolics extracted from Rosa multiflora were 12.08, 11.82, 11.1 and 12.6 mg/g when using water, 70% ethanol, 70% methanol and 70% acetone as the solvent, respectively. The optimum conditions for extracting the phenolic compounds were 70% ethanol over for 12 hrs(11.82 mg/g). The electron donating ability and inhibition rate on ABTS of the 70% ethanol extracts were 97% and 92.2%, respectively while the antioxidant protection factor(PF) of the water extracts and 70% ethanol extracts were 1.79 and 1.34 PF, respectively. The TBAR (thiobarbituric acid reactive substance) value were $1.3{\mu}M$ for the control and $0.15{\mu}M$ for the 70% ethanol extracts. The inhibitory activity against ${\alpha}$-amylase was 26% for the 70% ethanol extracts. The 70% ethanol extracts from Rosa multiflora Thunberg fruits exhibited antimicrobial activity against H. pylori, S. epidermidis, S. aureusand and E. coli with clear zone diameters of 14, 25, 14 and 13 mm, respectively when using $200{\mu}g/mL$ of the phenolic compounds. An HPLC analysis identified 6 major phenolic metabolites in the Rosa multiflora Thunberg fruits extracts: rosmarinic acid, caffeic acid, chlorogenic acid, courmaric acid, protocatechuic acid and quercetin. In particular, the content of rosmarinic acid was the highest in the 70% ethanol extracts. Therefore these results indicate that 70% ethanol extracts from Rosa multiflora Thunberg fruits can be useful as a natural antioxidant and in functional foods.
In this study, we selected some material to have potential bioactivity from natural plants, confirmed as basic data for industrializing and tried to develope the food materials using them. DPPH, ABTS, antioxidant protection factor, TBARs and antimicrobial activity of extracts from Rosa multiflora Thunberg fruits were determined. The total phenolics extracted from Rosa multiflora were 12.08, 11.82, 11.1 and 12.6 mg/g when using water, 70% ethanol, 70% methanol and 70% acetone as the solvent, respectively. The optimum conditions for extracting the phenolic compounds were 70% ethanol over for 12 hrs(11.82 mg/g). The electron donating ability and inhibition rate on ABTS of the 70% ethanol extracts were 97% and 92.2%, respectively while the antioxidant protection factor(PF) of the water extracts and 70% ethanol extracts were 1.79 and 1.34 PF, respectively. The TBAR (thiobarbituric acid reactive substance) value were $1.3{\mu}M$ for the control and $0.15{\mu}M$ for the 70% ethanol extracts. The inhibitory activity against ${\alpha}$-amylase was 26% for the 70% ethanol extracts. The 70% ethanol extracts from Rosa multiflora Thunberg fruits exhibited antimicrobial activity against H. pylori, S. epidermidis, S. aureusand and E. coli with clear zone diameters of 14, 25, 14 and 13 mm, respectively when using $200{\mu}g/mL$ of the phenolic compounds. An HPLC analysis identified 6 major phenolic metabolites in the Rosa multiflora Thunberg fruits extracts: rosmarinic acid, caffeic acid, chlorogenic acid, courmaric acid, protocatechuic acid and quercetin. In particular, the content of rosmarinic acid was the highest in the 70% ethanol extracts. Therefore these results indicate that 70% ethanol extracts from Rosa multiflora Thunberg fruits can be useful as a natural antioxidant and in functional foods.
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제안 방법
An HPLC analysis was carried out to identify the phenolic profiles of the Rosa multiflora extracts. The 6 major phenolic metabolites identified were rosmarinic acid, caffeic acid, chlorogenic acid, coumaric acid, protocatechuic acid and quercetin (Table 3).
대상 데이터
This study used Staphylococcus aureus (standard colony KCTC 1039), Candida albicans (standard colony KCTC 7965), Escherichia coli (standard colony KCTC 1039) and Streptococcus mutans (standard colony KCTC 3065) that cause cavities. The microorganisms were incubated on agar plates using an nutrient medium, Brain Heart medium, YM medium and Tryptic soy medium in a 37℃ incubator and CO2 incubator while maintaining air and nonair conditions for 24~48 hrs.
이론/모형
The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was measured using Blois method (Brand-Williams et al.1995). Briefly, 3 mL of 60 μM DPPH in ethanol was added to 1 mL of each sample and incubated at room temperature for 15 min.
The HPLC chromatographic analysis of the Rosa multiflora extracts was conducted using a modified version of the Whitaker’ method (Vattem and Shetty 2002; Whitaker et al. 1984).
The inhibition activity of pancreatin α-amylase was measured using an agar diffusing method (Cavidson and Parish 1989).
The measurement of the ABTS[2,2'-azinobis(3-ethylbenzothiazoline-6- sulfonic acid)] radical cationdecolorization was determined using the Pellegrin et al. method (Pellegrinet al. 1998).
The thiobarbituric acid substances(TBARs) were measured using the Buege and Aust' method (Buege and Aust 1978).
The total phenolic was measured according to the Folin-Denis' method (Joslyn 1970).
성능/효과
In conclusion, the Rosa multiflora extracts were identified and characterized with a high phenolic profile and high antioxidant and antimicrobial activity. Furthermore, the high phenolic profile and high antioxidant activity were clearly correlated with a high antimicrobial and α-amylase inhibitory activity.
02 μM. Therefore, the results indicated that Rosa multiflora extracts had a high inhibitory ability against oxidation. Thus, the antioxidant activities of the phenolic compounds in the Rosa multiflora extracts match previous reports of phenoilc compounds and flavonoids in plants acting, as natural antioxidants (Bors and Saran 1987; Fitzpatrick et al.
(1996) reported that thehigher the ability of electron donation, the higher the reducing ability of phenolic substances. To measure the hydrophilic antioxidant ability, the ABTS radical cation decolorization was assayed and no differences were found between the water extracts and the 70% ethanol extracts, as both exhibited high inhibition rates at 91.6% and 92.2%, respectively [Table 1]. Therefore it is anticipated that the water extracts and 70% ethanol extracts can both be developed as hydrophilic and lipophilic agents.
3). Yet, the current results were remarkably low when compared with those previously reported for Rubifructus extracts (84%) and Schizandrae fructus extracts (100%). Iwuoha and Aina (Iwuoha and Aina 1997) reported that, phenolic matter reduces the ability to resolve starch, thereby inhibiting the activity of α-amylase.
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