Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae원문보기
The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi....
The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.
The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.
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문제 정의
This result suggested that toxoflavin could be used as a selective medium for Fusarium species. This study showed that a medium containing toxoflavin was efficient for isolation of Fusarium species from grains and fields and can be widely used by researchers working on disease diagnosis, population genetics, and disease forecasting.
제안 방법
, 2004). The toxicity to eukaryotic cells and simple detoxification system led to the use of toxoflavin and the tflA gene as a positive selection system for plant transformation (Koh et al., 2011), and as a possible selection marker for Fusarium transformation in this study. However, all F.
대상 데이터
Isolation of fungal strains from air. MM medium containing 0, 20, and 80 mg toxoflavin/lwere added to 90 mm Petri dishes and the plates were exposed to a field of wheat at early anthesis stage in the Dong-A University Research Farm (Gyeongnam province, Korea) in May 2012. Three plates with each concentration of toxoflavin were left open for 1 h at three different locations ~100 m apart from each other in the wheat field.
데이터처리
The experiments were repeated three times with three replicates and Tukey test using SPSS 12.0 software (SPSS Inc., Chicago, USA) was performed to examine the significant differences (P < 0.05) of mycelia growth among the mean values of the samples.
성능/효과
Approximately three Fusarium strains per plate were isolated from all plates regardless of the toxoflavin concentration, while efficiently eliminating other saprophytic fungal species by adding toxoflavin to the medium. Consequently, 25%, 42%, and 88% of strains isolated from media containing 0, 20, and 80 mg toxoflavin/l, respectively, were Fusarium species (~95% and ~5% were F. graminearum lineage 6 and F. oxysporum, respectively).
, 2009). In that study, rice grains were highly contaminated by F. graminearum, and F. graminearum strains isolated from other crops such as maize and wheat also triggered severe FHB in rice, indicating that FHB in rice has been as severe as in wheat and barley grown in Korea. Therefore, it is possible for F.
2). In total, four fungal strains from 100 rice seeds were grown well and they all were identified as F. graminearum based on the morphological characteristics and TEF sequences.
참고문헌 (17)
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