[논문]Seahorse-derived peptide suppresses invasive migration of HT1080 fibrosarcoma cells by competing with intracellular α-enolase for plasminogen binding and inhibiting uPA-mediated activation of plasminogen

Kim, Yong-Tae; Kim, Se-Kwon; Jeon, You-Jin; Park, Sun Joo

doi:10.5483/bmbrep.2014.47.12.235

Seahorse-derived peptide suppresses invasive migration of HT1080 fibrosarcoma cells by competing with intracellular α-enolase for plasminogen binding and inhibiting uPA-mediated activation of plasminogen 원문보기

BMB reports, v.47 no.12, 2014년, pp.691 - 696

Kim, Yong-Tae (Department of Food Science and Technology, Kunsan National University) , Kim, Se-Kwon (Department of Chemistry, Pukyong National University) , Jeon, You-Jin (Department of Marine Life Sciences, Jeju National University) , Park, Sun Joo (Department of Chemistry, Pukyong National University)

Abstract ▼ AI-Helper

${\alpha}$-Enolase is a glycolytic enzyme and a surface receptor for plasminogen. ${\alpha}$-Enolase-bound plasminogen promotes tumor cell invasion and cancer metastasis by activating plasmin and consequently degrading the extracellular matrix degradation. Therefore, ${\alpha}$-enolase and plasminogen are novel targets for cancer therapy. We found that the amino acid sequence of a peptide purified from enzymatic hydrolysates of seahorse has striking similarities to that of ${\alpha}$-enolase. In this study, we report that this peptide competes with cellular ${\alpha}$-enolase for plasminogen binding and suppresses urokinase plasminogen activator (uPA)-mediated activation of plasminogen, which results in decreased invasive migration of HT1080 fibrosarcoma cells. In addition, the peptide treatment decreased the expression levels of uPA compared to that of untreated controls. These results provide new insight into the mechanism by which the seahorse-derived peptide suppresses invasive properties of human cancer cells. Our findings suggest that this peptide could emerge as a potential therapeutic agent for cancer.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Therefore, NF-KB is likely to be an important transcription regulator regulating uPA expression in invasive HT1080 cells. This finding provides important information to explore the role of peptide suppressing tumor progression.

가설 설정

(B) Peptide treatment decreases the association of α-enolase and plasminogen.
Therefore, in this study, we investigated whether the seahorse peptide influences the physiological role of endogenous α-enolase, such as binding and activation of plasminogen.

참고문헌 (20)

1 Takei N. Kondo J. Nagaike K. Ohsawa K. Kato K. Kohsaka S. Neuronal survival factor from bovine brain is identical to neuron-specific enolase. J. Neurochem. (1991) 57 1178 1184 10.1111/j.1471-4159.1991.tb08277.x 1895102

상세보기
2 Fluit A. C. Wolfhagen M. J. Jansze M. Torensma R. Verhoef J. Toxin B of Clostridium difficile does not have enolase activity. FEBS Lett. (1993) 316 103 105 10.1016/0014-5793(93)81745-L 8422931

상세보기
3 al-Giery A. G. Brewer J. M. Characterization of the interaction of yeast enolase with polynucleotides. Biochim. Biophys. Acta. (1992) 1159 134 140 10.1016/0167-4838(92)90017-8 1382613

상세보기
4 Vanegas G. Quinones W. Carrasco-Lopez C. Concepcion J. L. Albericio F. Avilan L. Enolase as a plasminogen binding protein in Leishmania mexicana. Parasitol. Res. (2007) 101 1511 1516 10.1007/s00436-007-0668-7 17653767

상세보기
5 Mundodi V. Kucknoor A. S. Alderete J. F. Immunogenic and plasminogen-binding surface-associated alpha-enolase of Trichomonas vaginalis. Infect. Immun. (2008) 76 523 531 10.1128/IAI.01352-07 18070902

상세보기
6 Plow E. F. Felez J. Miles L. A. Cellular regulation of fibrinolysis. Thromb. Haemost. (1991) 66 32 36 1656542
7 Plow E. F. Herren T. Redlitz A. Miles L. A. Hoover-Plow J. L. The cell biology of the plasminogen system. FASEB J. (1995) 9 939 945 7615163

상세보기
8 Wiman B. Collen D. On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin. J. Biol. Chem. (1979) 254 9291 9297 158022
9 Lancefield R. C. Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test. J. Exp. Med. (1957) 106 525 544 10.1084/jem.106.4.525 13475611

상세보기
10 Gentile F. Pizzimenti S. Arcaro A. Pettazzoni P. Minelli R. D'Angelo D. Mamone G. Ferranti P. Toaldo C. Cetrangolo G. Formisano S. Dianzani M. U. Uchida K. Dianzani C. Barrera G. Exposure of HL-60 human leukaemic cells to 4-hydroxynonenal promotes the formation of adduct(s) with alpha-enolase devoid of plasminogen binding activity. Biochem. J. (2009) 422 285 294 10.1042/BJ20090564 19508232

상세보기
11 Ryu B. Qian Z. J. Kim S. K. Purification of a peptide from seahorse, that inhibits TPA-induced MMP, iNOS and COX-2 expression through MAPK and NF-kappaB activation, and induces human osteoblastic and chondrocytic differentiation. Chem. Biol. Interact. (2010) 184 413 422 10.1016/j.cbi.2009.12.003 20004183

상세보기
12 Quinones W. Pena P. Domingo-Sananes M. Caceres A. Michels P. A. Avilan L. Concepcion J. L. Leishmania mexicana: molecular cloning and characterization of enolase. Exp. Parasitol. (2007) 116 241 251 10.1016/j.exppara.2007.01.008 17382932

상세보기
13 Pancholi V. Multifunctional alpha-enolase: its role in diseases. Cell. Mol. Life Sci. (2001) 58 902 920 10.1007/PL00000910 11497239

상세보기
14 Boyle M. D. Lottenberg R. Plasminogen activation by invasive human pathogens. Thromb. Haemost. (1997) 77 1 10 9031440
15 Coleman J. L. Benach J. L. Use of the plasminogen activation system by microorganisms. J. Lab. Clin. Med. (1999) 134 567 576 10.1016/S0022-2143(99)90095-1 10595783

상세보기
16 Lahteenmaki K. Edelman S. Korhonen T. K. Bacterial metastasis: the host plasminogen system in bacterial invasion. Trends. Microbiol. (2005) 13 79 85 10.1016/j.tim.2004.12.003 15680767

상세보기
17 Bergmann S. Wild D. Diekmann O. Frank R. Bracht D. Chhatwal G. S. Hammerschmidt S. Identification of a novel plasmin(ogen)-binding motif in surface displayed alpha-enolase of Streptococcus pneumoniae. Mol. Microbiol. (2003) 49 411 423 10.1046/j.1365-2958.2003.03557.x 12828639

상세보기
18 Ehinger S. Schubert W. D. Bergmann S. Hammerschmidt S. Heinz D. W. Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites. J. Mol. Biol. (2004) 343 997 1005 10.1016/j.jmb.2004.08.088 15476816

상세보기
19 Felez J. Chanquia C. J. Levin E. G. Miles L. A. Plow E. F. Binding of tissue plasminogen activator to human monocytes and monocytoid cells. Blood (1991) 78 2318 2327 1932747

상세보기
20 Del Pozo V. Rojo M. Rubio M. N. Cortegano I. Cardaba B. Gallardo S. Ortega M. Civantos E. Lopez E. Martin-Mosquero C. Peces-Barba G. Palomino P. Gonzalez-Mangado N. Lahoz C. Gene therapy with galectin-3 inhibits bronchial obstruction and inflammation in antigen-challenged rats through inerleukin-5 gene downregulation. Am. J. Respir. Crit. Care Med. (2002) 166 732 737 10.1164/rccm.2111031 12204873

상세보기

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증