[논문]피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과

임도연; 이경인

doi:10.7783/kjmcs.2014.22.6.457

피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과
Prostaglandin E2 Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.22 no.6, 2014년, pp.457 - 462

임도연 (광주여자대학교 교양교직과정부) , 이경인 (동신대학교 생물자원산업화지원센터)

Abstract ▼ AI-Helper

Prostaglandin (PG) $E_2$ is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, $PGE_2$ has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular $PGE_2$ levels in HaCaT cells and inhibited 15-PGDH ($ED_{50}$ : $168.4{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$ : $250.0{\mu}g/mL$). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE ($168.4{\mu}g/mL$) was comparable to that of TGF-${\beta}1$ (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of $PGE_2$ level dependent diseases.

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제안 방법

In this study, the ethanol extract of Eriobotryae folium (EFEE) as a 15-PGDH inhibitor after screening plant extracts was selected and investigated the regulation and expression of COX-1, COX-2, MRP4 and PGT in fibroblast cell line (HaCaT) for the regulation of PGE2 level.
Primers used for real-time PCR were as follows: Human PGT forward, 5’-GGATGCTGTTTGGAGGAATCCTCA-3’ and reverse, 5’-GCAC GATCCTGTCTTTGCTGAA-3’; MRP4 forward, 5’-AACCTCTAACC GACATTCCTG-3’ and reverse, 5’-TCAA CATATTACAGCCACCATC-3’; COX-1 forward 5’-CCTCAT GTTTGCCTTCTTTGC-3’ and reverse 5’-GGCGGGTACATT TCTCCATC-3’; COX-2 forward, 5’-GATCTACCCTCCTCA A-3’ and reverse 5’-GAACAACTGCTCATCAC-3’ and β-actin forward 5’-GACTATGACTTAGTTGCGTTA-3’ and reverse 5’-GTTGAAC TCTCTACATACTTCCG-3’.

이론/모형

Louis, MO, USA) and 100㎍/mL penicillin, in 5% CO₂ at 37℃. Cell viability was determined by the MTT assay. HaCaT cells (1 × 10⁴) were seeded in 96 well plates per 90 μL of DMEM medium.
On the other hand, cytotoxicity is very important for pharmaceutical application. The cytotoxicity of EFEE was determined by MTT assay. IC₅₀ (concentration for 50% of cell survival) of EFEE in HaCaT cells appeared was 250.

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연합인증

피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과
Prostaglandin E2 Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte 원문보기

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피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과 Prostaglandin E2 Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte 원문보기

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참고문헌 (29)

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피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과
Prostaglandin E2 Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte 원문보기

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