구강 캔디다증 알비칸스에 대한 프로 폴리스의 항균 활동 -프로폴리스를 이용한 미생물 억제 효과- Antimicrobial Activities of Propolis against Oral Candidiasis by Candida Albicans -Effect of Microbial Inhibition Using Propolis-원문보기
본 연구는 칸디다 알비칸스(C. albicans)에 대한 프로폴리스의 억제효과를 확인하고자 수행되었다. 프로폴리스는 양봉 꿀벌에서 채취하여 C. albicans는 $37^{\circ}C$에서 액체 배지에서 2시간 배양 하였다. 항균 활성검삼를 위해서 생리식염수 (PBS), 3% 차아염소산나트륨 (NaOCl에), 0.1% 클로로헥시딘 (CHX), 프로 폴리스 추출물 ($5{\mu}L/m{\ell}$, $10{\mu}L/m{\ell}$)에서 평가하였다. C. albicans는 3%의 NaOCl, 0.1% CHX, 프로 폴리스($5{\mu}L/m{\ell}$, $10{\mu}{\ell}/m{\ell}$)에서 15, 14.5, 16, 17mm의 억제구간을 확인하였다. 항균활성을 확인하기 위하여 집락형성을 분석한 결과, 3%의 NaOCl, 0.1 % CHX, $5{\mu}L/m{\ell}$와 $10{\mu}L/m{\ell}$의 프로 폴리스가 7, 7, 5, 7 로그지수가 감소를 보였다. C. albicans서는 $10{\mu}L/m{\ell}$의 프로폴리스에서 유의하게 억제되는 결과를 보여주었다. 이러한 결과를 통하여 프로폴리스는 구강 점막 질환에서 새로운 향균 약제가 될수 있는 것으로 생각된다.
본 연구는 칸디다 알비칸스(C. albicans)에 대한 프로폴리스의 억제효과를 확인하고자 수행되었다. 프로폴리스는 양봉 꿀벌에서 채취하여 C. albicans는 $37^{\circ}C$에서 액체 배지에서 2시간 배양 하였다. 항균 활성검삼를 위해서 생리식염수 (PBS), 3% 차아염소산나트륨 (NaOCl에), 0.1% 클로로헥시딘 (CHX), 프로 폴리스 추출물 ($5{\mu}L/m{\ell}$, $10{\mu}L/m{\ell}$)에서 평가하였다. C. albicans는 3%의 NaOCl, 0.1% CHX, 프로 폴리스($5{\mu}L/m{\ell}$, $10{\mu}{\ell}/m{\ell}$)에서 15, 14.5, 16, 17mm의 억제구간을 확인하였다. 항균활성을 확인하기 위하여 집락형성을 분석한 결과, 3%의 NaOCl, 0.1 % CHX, $5{\mu}L/m{\ell}$와 $10{\mu}L/m{\ell}$의 프로 폴리스가 7, 7, 5, 7 로그지수가 감소를 보였다. C. albicans서는 $10{\mu}L/m{\ell}$의 프로폴리스에서 유의하게 억제되는 결과를 보여주었다. 이러한 결과를 통하여 프로폴리스는 구강 점막 질환에서 새로운 향균 약제가 될수 있는 것으로 생각된다.
Propolis is an extremely safe natural antimicrobial substance that has been reported to have powerful antibacterial efficacy. The aim of this study was to evaluate the inhibitory effects of propolis against Candida albicans (C. albicans). Propolis was collected from the honey bee Apis mellifera. The...
Propolis is an extremely safe natural antimicrobial substance that has been reported to have powerful antibacterial efficacy. The aim of this study was to evaluate the inhibitory effects of propolis against Candida albicans (C. albicans). Propolis was collected from the honey bee Apis mellifera. The strain of C. albicans was cultivated overnight in liquid media incubated at $37^{\circ}C$. The antimicrobial activity was investigated using phosphate buffered saline (PBS), 3% sodium hypochlorite (NaOCl), 0.1% chorhexidine (CHX), and propolis extracts ($5{\mu}l/ml$, $10{\mu}l/ml$). C. albicans were sensitive to 3% NaOCl, 0.1% CHX, and propolis ($5{\mu}l/ml$, $10{\mu}l/ml$) with zones of inhibition of 15, 14.5, 16, and 17 mm, respectively. The CFU of PBS, 3% NaOCl, 0.1% CHX, $5{\mu}l/ml$ and $10{\mu}l/ml$ of propolis led a 1, 7, 7, 5 and 7-log reduction. Among the groups tested, C. albicans was most sensitive to $10{\mu}l/ml$ of propolis, which showed the largest inhibition zones. Therefore, propolis can be a new antimicrobial therapy for oral mucosa disease in traditional medicine.
Propolis is an extremely safe natural antimicrobial substance that has been reported to have powerful antibacterial efficacy. The aim of this study was to evaluate the inhibitory effects of propolis against Candida albicans (C. albicans). Propolis was collected from the honey bee Apis mellifera. The strain of C. albicans was cultivated overnight in liquid media incubated at $37^{\circ}C$. The antimicrobial activity was investigated using phosphate buffered saline (PBS), 3% sodium hypochlorite (NaOCl), 0.1% chorhexidine (CHX), and propolis extracts ($5{\mu}l/ml$, $10{\mu}l/ml$). C. albicans were sensitive to 3% NaOCl, 0.1% CHX, and propolis ($5{\mu}l/ml$, $10{\mu}l/ml$) with zones of inhibition of 15, 14.5, 16, and 17 mm, respectively. The CFU of PBS, 3% NaOCl, 0.1% CHX, $5{\mu}l/ml$ and $10{\mu}l/ml$ of propolis led a 1, 7, 7, 5 and 7-log reduction. Among the groups tested, C. albicans was most sensitive to $10{\mu}l/ml$ of propolis, which showed the largest inhibition zones. Therefore, propolis can be a new antimicrobial therapy for oral mucosa disease in traditional medicine.
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문제 정의
Dodwad et al showed that the study was to investigate the effectiveness of a propolis-containing mouthrinse in inhibition of plaque formation and improvement of gingival health.
By these reasons, propolis can use the bio-medical applications for antimicrobial effect. Therefore, the aim of this study was to evaluate the antimicrobial effect of propolis against C. albicans.
대상 데이터
The inhibition assays were performed in sterile 96-well plates, and the optical density(OD) was detected using micro plate reader(BioTek Instruments, Winooski, VT, USA) at the wavelength of 550 nm. All tests were performed in triplicate.
The strain of C. albicans(KCTC 7965/ATCC 10231) was used as the test organism. The strain of C.
데이터처리
, Armonk, NY, USA). The clean zone and logarithms of each C. albicans plate count were taken and analysis using one-way analysis of variance(ANOVA) to check the difference in bacterial inhibition among the groups. P value of <0.
이론/모형
Antimicrobial activity was investigated using ager diffusion method. Sterilized filter paper disc(Advantec Toyo Kaisha, Ltd) of 8 mm diameter were impregnated with 100 μl of PBS, 3% Sodium hypochlorite(NaOCl), 0.
성능/효과
According to the findings of the study presented here, it is concluded that the 10μl/ml propolis was superior to CHX in terms of bacterial elimination against C. albicans strain.
후속연구
Propolis agent for the present study showed a significant role related to oral diseases, further study is also required to identify the effect of propolis with the different Streptococcus mutans. Dodwad et al showed that the study was to investigate the effectiveness of a propolis-containing mouthrinse in inhibition of plaque formation and improvement of gingival health.
참고문헌 (42)
D. Nicodemo, J. D. De, R. H. Couto, E. Malheiros, Heney bee lines selected for high propolis production also have superior hygienic behaviors and increased honey and pollen stores. Genetics and Molecular Research, Vol.12, NO.4, pp.6931-6938, 2013. DOI: http://dx.doi.org/10.4238/2013.December.19.12
W. M. Thomso, Propolis, The Medical journal of Australia. Vol.153, No.11-12, pp.654, 1990.
F. Borrelli, P. Maffia, L. Pinto, A. Ianaro, A. Russo, F. Capasso et al, Phytochemical compounds involved in the anti-inflamatory effect of propolis extract. Fitoterapia, Vol.73, No.1, pp.53-63, 2002. DOI: http://dx.doi.org/10.1016/S0367-326X(02)00191-0
A. Kujungiev, I. Tsvetkova, Y. Serkedjieva, V. Bankova, R. Christov, S. Popov, Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology, Vol.64, No.3, pp.235-240, 1999. DOI: http://dx.doi.org/10.1016/S0378-8741(98)00131-7
N. Orsolic, I. Basic, Immunomodulation by water-soluble derivative of propolis: a factor of antitumor reactivity. Journal of Ethnopharmacology, Vol.84, No.2-3, pp. 265-273, 2003. DOI: http://dx.doi.org/10.1016/S0378-8741(02)00329-X
D. Skaba, T. Morawiec, M. Tanasiewicz, et al, Influence of the toothpaste with brazilian ethanol extract propolis on the oral cavity health. Evidence-Based Complementary and Alternative Medicine, Vol.2013, No.2013, pp.1155-1567, 2013.
T. Tatefuji, N. Izumi, T. Ohta, S. Arai, M. Ikeda, Kurimot, Isolation and identification of compounds from Brazilian propolis which enhance macrophage spreading and mobility. Biological & Pharmaceutical Bulletin, Vol.19, No.7, pp.966-970, 1996. DOI: http://dx.doi.org/10.1248/bpb.19.966
A. L. Hamedt, I. C. Ortiz, P. A. Garcia-Huertas, J. Saenz, A. C. Araujo, J. C. Mattos, M. A. Rodriguez-Gazquez, O. Triana-Chave, Cytotoxic, mutagenic and genotoxic evaluation of crude extracts and fractions from Piper jericoense with trypanocidal action. Acta Tropica, Vol.19, No.131, pp.92-97, 2013.
W. Wieckiewicz, M. Miernik, M. Wieckiewicz, T. Morawiec, Does propolis help to maintain oral health? Evid Based Complement Alternat Med, 35, pp.1062, 2013.
A. H. Acikelli, S. Gustmann, W. Bardenheuer, J. Klein, U. Dembinski, B. Kohl, K. T. Yip, A. Nazif, R. Stoll, D. Strumberg, D. Diaz-Carballo, Flavonoids isolated from Caribbean propolis show cytotoxic activity in human cancer cell lines. Int J Clin Pharmacol Ther, Vol.51, No.1, pp.51-53, 2013. DOI: http://dx.doi.org/10.5414/CPP51051
M. M. Viuda, N. Y. Ruiz, L. J. Fern L. J. Perez, Functional properties of honey. propolis, and royal jelly, J Food Sci, Vol.73, pp.117-124, 2008. DOI: http://dx.doi.org/10.1111/j.1750-3841.2008.00966.x
Noh SB, Evaluation of the anti-bacterial effect and cytotoxicity on Korean propolis, Chosun University, Korea, 2008.
J. B. Daleprane, D. S. Abdalla, Emerging roles of propolis: antioxidant, cardioprotective, and antiangiogenic actions. Evid Based Complement Alternat Med : Epub, pp.175-135, 2013.
A. K. Kuropatnicki, E. Szliszka, M. Klosek, W. Krol, The beginnings of modern research on propolis in poland. Evid Based Complement Alternat Med: Epub, Vol.983, pp.974, 2013.
C. O. Frozza, C. S. Garcia, G. Gambato, M. D. de Souza, et al, Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis. Food Chem Toxicol, Vol.52, pp.137-142. 2013. DOI: http://dx.doi.org/10.1016/j.fct.2012.11.013
M. C. Marcucci, Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, Vol.26, pp.83-99, 1995. DOI: http://dx.doi.org/10.1051/apido:19950202
Y. K. Park, M. H. Koo, J. A. S. Abreu, M. Ikegaki, J. A. Cury, P. L. Rosalen, Antimicrobial activity of propolis on oral microorganisms. Curr Microbiol, Vol.36, pp.24-28, 1998. DOI: http://dx.doi.org/10.1007/s002849900274
D. O. Kim, O. K. Y. Chun, Y. L. Kim, H. Y. Moon, C. Y. Lee, Quantification of polyphenolics and their antioxidant capacity in fresh, plums. J Agri. Food Chem, Vol.51. pp.6509-6515, 2003. DOI: http://dx.doi.org/10.1021/jf0343074
I. Kubo, Muroi H, Himejima M. Antimicrobial activity of Green tea flavor components and their combination effects. J Agri Food Chem, Vol. 40, pp. 245-248, 1992. DOI: http://dx.doi.org/10.1021/jf00014a015
M. Schifter, S. C. Yeoh, H. Coleman, A. Georgio, Oral mucosal diseases: the inflammatory dermatoses, Aust Dent J, Vol.55, No.1, pp.23-38, 2010. DOI: http://dx.doi.org/10.1111/j.1834-7819.2010.01196.x
P. A. Castro, V. L. Bom, N. A. Brown, R. S. Almeida, L. N. Ramalho, M. Savoldi, M. H. Goldman, A. A. Berretta, G. H. Goldman, Identification of the cell targets important for propolis-induced cell death in Candida albicans. Fungal Genet Biol, Vol.60, pp.74-86, 2013. DOI: http://dx.doi.org/10.1016/j.fgb.2013.07.001
L. P. Kantheti, B. Reddy, S. Ravikumar, C. H. Anuradha, P. Chandrasekhar, M. R. Rajeswari, Isolation, identification, and carriage of candidal species in PHLAs and their correlation with immunological status in cases with and without HAART. J Oral Maxillofac Pathol, Vol.16, No.1, pp.38-44, 2012. DOI: http://dx.doi.org/10.4103/0973-029X.92971
B. V. Ready, G. R. Sridhar, C. H. Anuradha, Chandrasekhar p, Lingamaneni KP, Malingnant melanoma of the mandibular gingive : rare occurrence. Indian J Dent Res, Vol.21, No.2, pp. 302-305, 2010. DOI: http://dx.doi.org/10.4103/0970-9290.66644
M. V. Martin, The use of fluconazole and itraconazole in the treatment of Candida albicans infections: a review. Journal of Antimicrobial Chemotherapy, Vol.44, No.4, pp. 429-437, 1999. DOI: http://dx.doi.org/10.1093/jac/44.4.429
J. Cuttner, K. M. Troy, L. Funaro, R. Brenden, E. J. Bottone, Clotrimazole treatment for prevention of oralcandidia is in patients with acute leukemia undergoing chemotherapy, Results of a double-blind study. Am J Med, Vol.81, No.5, pp.771-774, 1986. DOI: http://dx.doi.org/10.1016/0002-9343(86)90342-6
A. Bystrom, G. Sundqvist, Bacteriologic evaluation of the effect of 0.5 percent sodium hypochlorite in endodontic therapy. Oral Surg Oral Med Oral Pathol, Vol.55, No.3, pp.307-312, 1983. DOI: http://dx.doi.org/10.1016/0030-4220(83)90333-X
L. Grossman, B. Meiman, Solution of pulp tissue by chemical agents. J Am Dent Assoc, Vol.8, pp. 223-225, 1982.
E. Pashley, N. Birdsong, K. Bowman, D. Pashley, Cytotoxic effects of NaOCl on vital tissue. J Endod, Vol.11, No.12, pp.525-528, 1985. DOI: http://dx.doi.org/10.1016/S0099-2399(85)80197-7
O. Fardak, R. Turnbull, A review of literature on use of chlorhexidine in dentistry. J Am Dent Assoc, Vol.112, No.16, pp.863-869, 1985.
G. Greenstein, C. Berman, J. R. Chlorhexidine, An adjunct to periodontal therapy. J Periodontol, Vol.57, No.6, pp.370-376, 1986. DOI: http://dx.doi.org/10.1902/jop.1986.57.6.370
M. Jeansonne, R. White, A comparison of 2.0% chlorhexidine gluconate and 5.25% sodium hypochlorite as antimicrobial endodontic irrigants. J Endod, Vol.20, No.6, pp.276-278, 1994. DOI: http://dx.doi.org/10.1016/S0099-2399(06)80815-0
C. B. Walker, The acquisition of antibiotic resistance in the periodontal microflora. Periodontol 2000, Vol.10, pp.79-88, 1996. DOI: http://dx.doi.org/10.1111/j.1600-0757.1996.tb00069.x
T.A. Kost, Expression vectors and delivery systems tools for determining gene function and gene therapy. Curr Opin Biotechnol, Vol.10, No.5, pp.409-10, 1999. DOI: http://dx.doi.org/10.1016/S0958-1669(99)00001-4
C. B. Walker, The acquisition of antibiotic resistance in the periodontal microflora. Periodontology 2000, Vol.10, pp.79-88, 1996. DOI: http://dx.doi.org/10.1111/j.1600-0757.1996.tb00069.x
E. L. Ghisalberti, Propolis: a review, Bee World. Vol.60, No.2, pp.59-84, 1960.
Y. K. Park, M. H. Koo, J. A. Abre, M. Ikegaki, J. A. Cury, P. L. Rosalen, Antimicrobial activity of propolis on oral microorganisms. Current Microbiology, Vol.36, No.1, pp.2428, 1998. DOI: http://dx.doi.org/10.1007/s002849900274
G. A. Burdock, Review of the Biological properties and toxicity of bee propolis(propolis). Food Chem Toxicol, Vol.36, No.4, pp. 347-363, 1998. DOI: http://dx.doi.org/10.1016/S0278-6915(97)00145-2
S. A. Duailibe, A. G. Goncalves, F. J. Ahid, Effect of a propolis extract on Streptococcus mutans counts in vivo. J Appl Oral Sci, Vol.15, No.5, pp.420-423 2007. DOI: http://dx.doi.org/10.1590/S1678-77572007000500009
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