글리세릴 베이스의 신규베지클 이용 캡슐화를 통한 항산화성 아스타잔틴의 성질 강화 The Cosmeceutical Property of Antioxidant Astaxanthin is Enhanced by Encapsulation Using Glyceryl Based New Vesicle원문보기
새로운 베지클인 glyceryl citrate/ lactate/ linoleate/ oleate를 이용한 수중유형 형태의 아스타잔틴나노에멀젼에 대해 항산화 효과, 세포 생존력, 단백질과 관련한 효소의 영향, 피부 침투도 그리고 피부에 대한 보습 및 탄력 등의 약용화장품적인 측면에서의 전반적 연구를 실시하였다. 항산화력 및 세포 생존력에 대해선 각각 DPPH법과 MMT assay를 이용하여 측정하였다. 아스타잔틴 나노에멀젼에 대한 또 다른 성질은 2D-Page를 이용한 단백질 분석 및 컨포칼, in-vivo 테스트를 통해 측정하였다. 본 연구를 통해, 아스타잔틴을 포함하는 나노에멀젼은 MMP발현에 관련한 단백질 억제 및 세포외 기질의 분해를 막고 라디칼의 소거에 매우 우수한 결과를 보였다. 종전의 레시친을 이용한 나노에멀젼 보다는 새로운 베지클을 이용한 아스타잔틴 나노에멀젼의 피부 침투가 매우 효과적임을 CLSM을 통해 측정하였다. 또한 28일 동안의 한국 성인 여성 11명을 통한 보습 및 탄력 인비보 테스트에서 우수한 효과를 확인할 수 있었다.
새로운 베지클인 glyceryl citrate/ lactate/ linoleate/ oleate를 이용한 수중유형 형태의 아스타잔틴 나노에멀젼에 대해 항산화 효과, 세포 생존력, 단백질과 관련한 효소의 영향, 피부 침투도 그리고 피부에 대한 보습 및 탄력 등의 약용화장품적인 측면에서의 전반적 연구를 실시하였다. 항산화력 및 세포 생존력에 대해선 각각 DPPH법과 MMT assay를 이용하여 측정하였다. 아스타잔틴 나노에멀젼에 대한 또 다른 성질은 2D-Page를 이용한 단백질 분석 및 컨포칼, in-vivo 테스트를 통해 측정하였다. 본 연구를 통해, 아스타잔틴을 포함하는 나노에멀젼은 MMP발현에 관련한 단백질 억제 및 세포외 기질의 분해를 막고 라디칼의 소거에 매우 우수한 결과를 보였다. 종전의 레시친을 이용한 나노에멀젼 보다는 새로운 베지클을 이용한 아스타잔틴 나노에멀젼의 피부 침투가 매우 효과적임을 CLSM을 통해 측정하였다. 또한 28일 동안의 한국 성인 여성 11명을 통한 보습 및 탄력 인비보 테스트에서 우수한 효과를 확인할 수 있었다.
Oil-in-water nanoemulsions of astaxanthin prepared by new vesicle, glyceryl citrate/ lactate/ linoleate/ oleate, were evaluated thoroughly in terms of cosmeceutical properties such as antioxidant effect, cell viability, influence of protein related enzyme, skin penetration, skin hydration and elasti...
Oil-in-water nanoemulsions of astaxanthin prepared by new vesicle, glyceryl citrate/ lactate/ linoleate/ oleate, were evaluated thoroughly in terms of cosmeceutical properties such as antioxidant effect, cell viability, influence of protein related enzyme, skin penetration, skin hydration and elasticity. Antioxidant effect and cell viability of nanoemulsion of astaxanthin were evaluated by DPPH and MTT assay. Also other properties of nanoemulsions of astaxanthin were measured by proteome analysis using 2D-PAGE, confocal laser scanning microscope and in-vivo test. We were able to find that the nanoemulsion of astaxanthin is good at scavenging of radical and inhibits the degradation of dermal extracellular matrix with the down-regulation of MMPs and other proteins related to MMP expression. CLSM was adopted for observing penetration of nanoemulsion of astaxanthin and showed high effective penetration rate compared to the nanoemulsion of astaxanthin prepared by conventional lecithin. In-vivo measurement of the nanoemulsions in hydration and elasticity were conducted to 11 Korean female adults for 28 days and showed better results.
Oil-in-water nanoemulsions of astaxanthin prepared by new vesicle, glyceryl citrate/ lactate/ linoleate/ oleate, were evaluated thoroughly in terms of cosmeceutical properties such as antioxidant effect, cell viability, influence of protein related enzyme, skin penetration, skin hydration and elasticity. Antioxidant effect and cell viability of nanoemulsion of astaxanthin were evaluated by DPPH and MTT assay. Also other properties of nanoemulsions of astaxanthin were measured by proteome analysis using 2D-PAGE, confocal laser scanning microscope and in-vivo test. We were able to find that the nanoemulsion of astaxanthin is good at scavenging of radical and inhibits the degradation of dermal extracellular matrix with the down-regulation of MMPs and other proteins related to MMP expression. CLSM was adopted for observing penetration of nanoemulsion of astaxanthin and showed high effective penetration rate compared to the nanoemulsion of astaxanthin prepared by conventional lecithin. In-vivo measurement of the nanoemulsions in hydration and elasticity were conducted to 11 Korean female adults for 28 days and showed better results.
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제안 방법
Comparison between nanoemulsions with or without 1% of AX extract for radical scavenging property was performed. And also other antioxidant agents such as ascorbic acid, berry complex (Vitalizing Code B), magnesium ascorbyl phosphate (VC-PMC), tocopherol, ellagic acid, hydroxy dimethoxybenzyl malonate (Ronacare AP) were used as a co-antioxidant for additional comparison.
In-vivo measurement of hydration and elasticity were conducted by testing 11 Korean female adults for 28 days by using corneometer CM825 and cutometer MPA580. Statistical analysis of hydration was conducted using a program named SPSSR 14.
In-vivo measurement of hydration and elasticity were conducted by testing 11 Korean female adults for 28 days by using corneometer and cutometer.
Sample was spreaded on the skin tissue sized 25 × 25 mm after removing moisture. Measurement of skin penetration was conducted in 10, 30, 60, 120 min at 514 nm using confocal laser scanning microscope after sampling on the slide glass by using cryo-microtome (Lecia CM 1850, Germany). Thickness of sample was 20 µm.
Human dermal fibroblast cells (ATCC, CRL-1635, American Type Culture Collection, USA) were purchased to evaluate cell viability. Proteome analysis was performed with IPGphore (EttanTM 3 IEF, Amersham bioscience, Sweden) and image master (ImageMsterTM 2D Elite, Amersham biosciences, Sweden) to evaluate influence of protein related enzyme activity. CLSM (Confocal Laser Scanning Microscopy, Leica microsystem TCS SP5, Germany) was adopted for checking penetration of nanoemulsion of AX.
The purpose of this study is to evaluate the cosmeceutical properties such as antioxidant effect, cell viability, influence of protein related enzyme, skin penetration, hydration and elasticity on nanoemulsion of AX.
대상 데이터
2-D PAGE is the separation method for proteins with high resolution. Immobiline Dry strips (24 cm, pI 4-7NL) were used with an Ettan IPGphore fixed-length strip holder. The strip was rehydrated for 12 h with rehydration solution and the sample proteome was injected simultaneously.
데이터처리
In-vivo measurement of hydration and elasticity were conducted by testing 11 Korean female adults for 28 days by using corneometer CM825 and cutometer MPA580. Statistical analysis of hydration was conducted using a program named SPSSR 14.0. Figure 6 shows increase of hydration on nanoemulsion of AX with glyceryl citrate/lactate/linoleate/oleate formulated by Table 1.
이론/모형
The supernatant was collected and preserved at −20 ℃. The amount of total protein extracted was measured via the modified Bradford method.
성능/효과
In this study, 20 ~ 50% decrease of interleukin 1-alpha (IL-1α), TNF-α, NFkappa B were observed.
Among them, the MMP1 has played an important role in the remodeling of connective tissue while MMP2 and MMP3 decompose the elastic quality and matrix protein such as pro teoglycan, fibronectin and laminin[17]. The decrease of proteins such as MMP1, MMP2, MMP3, MMP9 and MMP14 were found from the analysis and thought to be influenced by MMP expressing proteins. Except them, there are several proteinases such as Cathepsin, cathepsin precursor and elastase IIIB.
참고문헌 (17)
W. Miki, Biological functions and activities of animal carotenoids, Pure Appl. Chem., 63, 141 (1989).
N. Shimizu, M. Goto, and W. Miki, Carotenoids as singlet oxygen quenchers in marine organisms, Fish. Sci., 62, 134 (1996).
X. W. Zhang, X. D. Gong, and F. Chen, Kinetic models for astaxanthin production by high cell density mixotrophic culture of the microalga Haematococcus pluvialis, J. Ind. Microbiol. Biotechnol., 23, 691 (1999).
I. H. Ciapara, L. F. Valenzuela, F. M. Goycoolea, and W. A. Monal, Microencapsulation of astaxanthin in a chitosan matrix, Carbohydr. Polym., 56, 41 (2004).
I. Amar, A. Aserin, and N. Garti, Solubilization patterns of lutein and lutein esters in food grade nonionic microemulsions, J. Agric. Food Chem., 51, 4775 (2003).
A. Spernath, A. Yaghmur, A. Aserin, R. E. Hoffman, and N. Garti, Food-grade microemulsions based on nonionic emulsifiers: media to enhance lycopene solubilization, J. Agric. Food Chem., 50, 6917 (2002).
E. Yilmaz and H. H. Borchert, Effect of lipid containing, positively charged nanoemulsions on skin hydration, elasticity and erythema - an in vivo study, Int. J. Pharmaceutics, 307, 232 (2006).
D. M. Kim, W. K. Hong, S. S. Kong, and U. Y. Lee, Novel encapsulation with new glyceryl ester vesicle enhances stability of nanoemulsion containing astaxanthin, J. Soc. Cosmet. Scientists Korea, 39(3), 225 (2013).
A. Gorg, C. Obermaier, G. Boguth, A. Harder, B. Scheibe, R. Wildgruber, and W. Weiss, Current two-dimensional electrophoresis technology for proteomics, Electrophoresis, 21, 1037 (2000).
M. M Sanders and E. T. Broconing, Solubilization of proteins in 2D electrophoresis: An Outline, Anal. Biochem., 103, 157 (1980).
B. Herbert, M. P. Molloy, and K. L. Williams, Improved protein solubility in two -dimensional electrophoresis using tributyl phosphine as reducing agent, Electrophoresis, 19, 845 (1998).
B. S. Yoo, M. A. Yoo, Y. K Song, and S.Y. Byun, Regulation of proteins related to melanogenesis by heartwood extract of morus ombycis using proteome analysis, Biotechnol. Bioprocess Eng., 12, 662 (2007).
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