Antifungal Activities of Crude Extractum from Camellia semiserrata Chi (Nanshancha) Seed Cake Against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo Fruit Test원문보기
Meng, Xiangchun
(Institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture)
,
Li, Jun
(Institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture)
,
Bi, Fangcheng
(Institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture)
,
Zhu, Lixue
(Zhongkai University of Agriculture and Engineering)
,
Ma, Zhiyu
(Zhongkai University of Agriculture and Engineering)
Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effec...
Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit.
Antifungal activities of crude extractum of Nanshancha Seed Cake (NSC), to inactivate postharvest pathogens were investigated. Highest inhibitory rate was found against C. musae, C. gloeosporioides and C. papaya P.Henn, which was much stronger than that by tea saponin. Compared to tea saponin, effects of NSC extractum was relatively weak and similar on C. gloeosporioides Penzig and P. italicum. In an in vivo study, best controlling effects by NSC extractum was found with banana anthracnose disease development, which showed no inhibitory effects by tea saponin. NSC extractum controlled in vitro C. musae growth through directly inhibiting germination rate and germ tube elongation, and causing distortation, rupture and indentation of C. musae mycelium. In banana fruit subject to C. musae inoculation, higher PAL, POD, GLU and CHT activity was observed in banana fruit treated with crude NSC extractum than that of water control fruits. Current study proved the best controlling effects of crude NSC extractum in C. musae in vitro and in vivo development, which through direct inhibition of C. musae growth and increasing defense system of the banana fruit.
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제안 방법
In this study, antifungal performance of Nanshacha seed cake extractum was investigated, tea saponin with the purity of 90% as a parallely control to prove if tea saponin is also a main component of the extractum. In addition, in vivo antifungal activity and defense ability change was subsequently examined on the three and one kind of fruits respectively, to help to understand the antifungal activity and its possible inhibitory mechanism.
대상 데이터
Fruits were again kept at ambient for drying before dipping into NSC or tea saponin solution at a different corresponding concentration respectively. Each dip treatment consisted of 30 fruits, i.e., 60 inoculation replicates per treatment. Treated fruit were packed into fruit cartons, covered with PE bag and stored at 25℃ and 95% relative humidity.
After 7 days, mycelial diameter was measured with digital calipers, and results were expressed as percentage inhibition rate, which was calculated using the formula: %Inhibition=100 (Control – Treatment)/Control. The experiment was carried out with 10 replicates per test.
데이터처리
All the experiments were designed in a completely randomized fashion with three replications and data were subjected to analyses of variance (ANOVA) using SPSS software. Mean comparisons were performed by Fisher’s Protected Least Significant Difference (LSD) test (P = 0.
Mean comparisons were performed by Fisher’s Protected Least Significant Difference (LSD) test (P = 0.05).
성능/효과
As shown in Fig. 2, obvious inhibition in the germination rate of C. musae and germ tube elongation was observed in the plates with NSC extractum, in which germination rate and germ tube elongation was markedly inhibited by 88.7% and 68.4% respectively at 0.5 mg/ml, 100% completely inhibition was observed with NSC extractum above 1 mg/ml.
1B). In Shatang mandarin inoculated by P. italicum, compared to non-treated control, 50 mg/ml NSC extractum treatment reduced the disease index by about 49% after 4 days of storage, but after 6 and 8 days of storage, NSC extractum was less effective against the P. italicum infection. Tea saponin provided 13% controlling effects of the mold decay development on Shatang mandarin at 4 days of storage, but less significant disease control reduction was observed after 6 and 8 days of storage (Fig.
, 2005). In this study, antifungal performance of Nanshacha seed cake extractum was investigated, tea saponin with the purity of 90% as a parallely control to prove if tea saponin is also a main component of the extractum. In addition, in vivo antifungal activity and defense ability change was subsequently examined on the three and one kind of fruits respectively, to help to understand the antifungal activity and its possible inhibitory mechanism.
, 2012). Results in this text indicates that other active antifungal components existing in the crude extractum other than tea saponin, and the antifungal activity of our NSC extractum is the synergistic effects of more than one compound. Further research is required to separate these active components and validate their anti-fungal activity.
Results showed that the anthracnose lesion diameter of non-treated banana fruit (control, CK) gradually developed to 2.8 mm, 6.6 mm and 14.6 mm after 6th day, 9th day and 12th day respectively, tea saponin with the concentration of 5 mg ml-1 proved no inhibitory effects against anthracnose decay development. 5 mg/ml NSC extractum provided completely control of anthracnose decay development after 6and 9 days of storage, and after 12 days of storage, the lesion diameter was reduced by 83% compared to the control (Fig.
musae were 100%, but the concentration of tea saponin (5 mg/ml) is five folds than that of NSC extractum (1 mg/ml). The inhibition rate of the extractum under the same concentration of 5 mg/ml against mango and papaya C. gloeosporioides was 90.9% and 85.3% respectively, much strong inhibitory effect in comparison with that of tea saponin, which was 49.8% and 41.3% respectively. The inhibition rate of the 50 mg/ml NSC extractum and tea saponin against guava C.
후속연구
Results in this text indicates that other active antifungal components existing in the crude extractum other than tea saponin, and the antifungal activity of our NSC extractum is the synergistic effects of more than one compound. Further research is required to separate these active components and validate their anti-fungal activity.
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