[논문]A Novel Lactobacillus casei LP1 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Stimulator

Kang, Jo-Eun; Kim, Tae-Jung; Moon, Gi-Seong

doi:10.3746/pnf.2015.20.1.78

A Novel Lactobacillus casei LP1 Producing 1,4-Dihydroxy-2-Naphthoic Acid, a Bifidogenic Growth Stimulator 원문보기

Preventive nutrition and food science, v.20 no.1, 2015년, pp.78 - 81

Kang, Jo-Eun (Department of Biotechnology, Korea National University of Transportation) , Kim, Tae-Jung (Department of Biotechnology, Korea National University of Transportation) , Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation)

Abstract ▼ AI-Helper

1,4-Dihydroxy-2-naphthoic acid (DHNA) is a bifidogenic growth stimulator (BGS) and could be a functional food ingredient since bifidobacteria are beneficial for human health. For that reason, lactic acid bacteria producing DHNA have been screened. A lactic acid bacterium LP1 strain isolated from a natural cheese was confirmed to produce DHNA, analyzed by a HPLC method. The strain was identified as Lactobacillus casei by 16S rRNA gene sequence analysis. The cell-free supernatant of fermented whey produced by L. casei LP1 presented the BGS activity for three bifidobacterial strains such as Bifidobacterium longum subsp. infantis KCTC 3127, Bifidobacterium bifidum KCTC 3202, and Bifidobacterium breve KCTC 3220 which were human-originated. To the best of our knowledge, a L. casei strain which can produce DHNA was firstly identified in this study.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

대상 데이터

The flow rate was 1 mL/min, injection volume was 20 μL, and detection wavelength was 254 nm. Reagent grade DHNA (Sigma-Aldrich) was used for the standard material. The mobile phase was composed of acetonitrile : methanol : water : acetic acid (15:25:225:0.
The primer set 27F (5'-AGA GTT TGA TCC TGG CTC AG-3') and 1492R (5'-TAC GGY TAC CTT GTT ACG ACT T-3') was used for amplification of the 16S rRNA gene and 518F (5'-CCA GCA GCC GCG GTA ATA CG-3') and 800R (5'-TAC CAG GGT ATC TAA TCC-3') primer set was used for sequencing.
longum subsp. infantis KCTC 3127, B. bifidum KCTC 3202, and B. breve KCTC 3220, respectively. From these results, we concluded that the fermented whey with L.
Bifidobacterial strains including Bifidobacterium longum subsp. infantis KCTC 3127, Bifidobacterium bifidum KCTC 3202, and Bifidobacterium breve KCTC 3220 were purchased from the Korean Collection for Type Cultures (KCTC) at Korea Research Institute of Bioscience and Biotechnology (KRIBB, Daejeon, Korea). They were cultured in Reinforced Clostridial Medium (RCM) (Difco Laboratories Inc.

이론/모형

The primer set 27F (5'-AGA GTT TGA TCC TGG CTC AG-3') and 1492R (5'-TAC GGY TAC CTT GTT ACG ACT T-3') was used for amplification of the 16S rRNA gene and 518F (5'-CCA GCA GCC GCG GTA ATA CG-3') and 800R (5'-TAC CAG GGT ATC TAA TCC-3') primer set was used for sequencing. Homology search for the sequence was performed using a Basic Local Alignment Search Tool (BLAST) program from The National Center for Biotechnology Information (NCBI; http://www.ncbi.nlm. nih.gov/).

성능/효과

breve KCTC 3220, respectively. From these results, we concluded that the fermented whey with L. casei LP1 producing DHNA could stimulate the growth of bifidobacterial strains, and the functional L. casei LP1 can be used as a probiotic or a prebiotic producer.
However, probiotics including bifidobacteria and lactobacilli can modulate human gut microbiota positively; therefore, they can prevent or improve the human gut pathogenesis (13). In this study, a novel Lactobacillus casei strain that produces DHNA was isolated, and the fermented whey with the strain was confirmed to stimulate the growth of bifidobacterial strains.
The strain was identified by 16S rRNA gene sequence analysis. The 16S rRNA gene sequence (GenBank accession no., KP696456) of LP1 was best matched with L. casei strains (99% identity; GenBank accession no. CP002618.1, CP002616.1, CP001084.1, FM177140.1, and CP000423.1), and the LP1 strain was named L. casei LP1. DHNA production from L.

참고문헌 (14)

1 Isawa K Hojo K Yoda N Kamiyama T Makino S Saito M Sugano H Mizoguchi C Kurama S Shibasaki M Endo N Sato Y 2002 Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3 Biosci Biotechnol Biochem 66 679 681 10.1271/bbb.66.679 12005071

상세보기
2 Sanders ME Lenoir-Wijnkoop I Salminen S Merenstein DJ Gibson GR Petschow BW Nieuwdorp M Tancredi DJ Cifelli CJ Jacques P Pot B 2014 Probiotics and prebiotics: prospects for public health and nutritional recommendations Ann NY Acad Sci 1309 19 29 10.1111/nyas.12377 24571254

상세보기
3 Okada Y Tsuzuki Y Miyazaki J Matsuzaki K Hokari R Komoto S Kato S Kawaguchi A Nagao S Itoh K Watanabe T Miura S 2006 Propionibacterium freudenreichii component 1,4-dihydroxy-2-naphthoic acid (DHNA) attenuates dextran sodium sulphate induced colitis by modulation of bacterial flora and lymphocyte homing Gut 55 681 688 10.1136/gut.2005.070490 16299037

상세보기
4 Okada Y Tsuzuki Y Narimatsu K Sato H Ueda T Hozumi H Sato S Hokari R Kurihara C Komoto S Watanabe C Tomita K Kawaguchi A Nagao S Miura S 2013 1,4-Dihydroxy-2-naphthoic acid from Propionibacterium freudenreichii reduces inflammation in interleukin-10-deficient mice with colitis by suppressing macrophage-derived pro-inflammatory cytokines J Leukoc Biol 94 473 480 10.1189/jlb.0212104 23801651

상세보기
5 Bentley R Meganathan R 1982 Biosynthesis of vitamin K (menaquinone) in bacteria Microbiol Rev 46 241 280 281544 --> 6127606

상세보기
6 Myers CR Myers JM 1993 Role of menaquinone in the reduction of fumarate, nitrate, iron(III) and manganese(IV) by Shewanella putrefaciens MR-1 FEMS Microbiol Lett 114 215 222 10.1111/j.1574-6968.1993.tb06576.x

상세보기
7 Newman DK Kolter R 2000 A role for excreted quinones in extracellular electron transfer Nature 405 94 97 10.1038/35011098 10811225

상세보기
8 Saffarini DA Blumerman SL Mansoorabadi KJ 2002 Role of menaquinone in Fe(III) reduction by membrane fraction of Shewanella putrefaciens J Bacteriol 184 846 848 10.1128/JB.184.3.846-848.2002 139511 --> 11790756

상세보기
9 Rohmer M Knani M Simonin P Sutter B Sahm H 1993 Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate Biochem J 295 517 524 1134910 --> 8240251

상세보기
10 Rohmer M Seemann M Horbach S Bringer-Meyer S Sahm H 1996 Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis J Am Chem Soc 118 2564 2566 10.1021/ja9538344

상세보기
11 Schwender J Seemann M Lichtenthaler HK Rohmer M 1996 Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus Biochem J 316 73 80 1217352 --> 8645235
12 Tojo R Suárez A Clemente MG de los Reyes-Gavilán CG Margolles A Gueimonde M Ruas-Madiedo P 2014 Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis World J Gastroenterol 20 15163 15176 10.3748/wjg.v20.i41.15163 4223251 --> 25386066

상세보기
13 Plaza-Diaz J Gomez-Llorente C Fontana L Gil A 2014 Modulation of immunity and inflammatory gene expression in the gut, in inflammatory diseases of the gut and in the liver by probiotics World J Gastroenterol 20 15632 15649 10.3748/wjg.v20.i42.15632 4229528 --> 25400447

상세보기
14 Furuichi K Katakura Y Ninomiya K Shioya S 2007 Enhancement of 1,4-dihydroxy-2-naphthoic acid production by Propionibacterium freudenreichii ET-3 fed-batch culture Appl Environ Microbiol 73 3137 3143 10.1128/AEM.01307-06 1907098 --> 17369348

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증