[논문]Analysis of Fungicide Sensitivity and Genetic Diversity among Colletotrichum Species in Sweet Persimmon

Gang, Geun-Hye; Cho, Hyun Ji; Kim, Hye Sun; Kwack, Yong-Bum; Kwak, Youn-Sig

doi:10.5423/ppj.oa.03.2015.0033

Analysis of Fungicide Sensitivity and Genetic Diversity among Colletotrichum Species in Sweet Persimmon 원문보기

The plant pathology journal, v.31 no.2, 2015년, pp.115 - 122

Gang, Geun-Hye (Division of Applied Life Science, Gyeongsang National University) , Cho, Hyun Ji (Division of Applied Life Science, Gyeongsang National University) , Kim, Hye Sun (Division of Applied Life Science, Gyeongsang National University) , Kwack, Yong-Bum (Namhae Sub-Station, NIHHS, RDA) , Kwak, Youn-Sig (Division of Applied Life Science, Gyeongsang National University)

Abstract ▼ AI-Helper

Anthracnose, caused by Colletotrichum gloeosporioides (C. gloeosporioides; Teleomorph: Glomerella cingulata), is the most destructive disease that affects sweet persimmon production worldwide. However, the biology, ecology, and genetic variations of C. gloeosporioides remain largely unknown. Therefore, in this study, the development of fungicide resistance and genetic diversity among an anthracnose pathogen population with different geographical origins and the exposure of this population to different cultivation strategies were investigated. A total of 150 pathogen isolates were tested in fungicide sensitivity assays. Five of the tested fungicides suppressed mycelial pathogen growth effectively. However, there were significant differences in the sensitivities exhibited by the pathogen isolates examined. Interestingly, the isolates obtained from practical management orchards versus organic cultivation orchards showed no differences in sensitivity to the same fungicide. PCR-restriction fragment length polymorphism (RFLP) analyses were performed to detect internal transcribed spacer regions and the ${\beta}$-tubulin and glutamine synthetase genes of the pathogens examined. Both the glutamine synthetase and ${\beta}$-tubulin genes contained a complex set of polymorphisms. Based on these results, the pathogens isolated from organic cultivation orchards were found to have more diversity than the isolates obtained from the practical management orchards.

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문제 정의

The primary objective of this study was to elucidate the relationship between anthracnose pathogen genetic diversity and farming management strategy of sweet persimmon. Based on the species-specific PCR results, 156 isolates were identified as C.

제안 방법

Sensitivity or tolerance of all of the tested isolates was evaluated based on the mean growth value±standard deviation (SD).

이론/모형

Genomic DNA was extracted from fresh mycelium by the CTAB method (Ford et al., 2000). DNA from each isolate was amplified using the following speciesspecific primer pairs: Colg1 (5′-AACCCTTTGTGAACATACC-3′) and Colg2 (5′-CCCTCCGGATCCCAG-3′) (443-bp) for C.
The fragmented products were scored according to the presence (score of 1) and absence (score of 0) of particular bands. These distances were used to cluster the isolates according to the unweighted pair group method with averages (UPGMA) to determine genetic distances among the isolates (Nei et al., 1973). A phylogenetic tree was generated using the XLSTAT program (www.

성능/효과

In conclusion, there were no significant differences in the fungicide sensitivity of the isolates that were obtained from orchards maintained with a practical management strategy versus an organic management strategy. These results suggest that disease control is mainly determined by whether a compatible fungicide has been used or not.
In the present study, ITS PCRRFLP phylogenetic trees showed no significant difference between the practical orchard and organic orchard isolates. Taken together, these results suggest that the ITS region may not provide sufficient information to classify the Colletotrichum population, or even the 22 species in the genus, Colletotrichum.
Among the four restriction enzyme assays, MspI and HaeIII provided clearer resolution patterns than those observed following the digests with AluI and TaqI, regardless of the genes involved. The PCR-RFLP results showed that the isolates from the organic orchards exhibit more diverse patterns at the genetic level than the isolates from the practical orchards.

후속연구

gloeosporioides population. However, additional studies of the Colletotrichum genus and other fungi were needed to confirm the precision and stability of the TubGF1 and TubGR primers. In the present study, β-tubulin PCR-RFLP and a phylogenetic analysis found no significant differences among the sweet persimmon anthracnose pathogens examined.

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