선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
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선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Seven days after the sensitization by intratracheal injection with OVA, the normal group was exposed to aerosolized PBS and the OVA control group was exposed to aerosolized OVA for 30 min/day, 3 days/week, for 3 weeks, in a polycarbonate chamber (40 ㎝ × 25 ㎝ × 27 ㎝).
- Seven days after the second challenges, intratracheal injection of 100 μL (250 ㎍/ mL) of OVA (on day 14) were administered in the back of the tongue. Starting from the 3rd week (on day 21), OVA solution was administered into the nasal cavity and the respiratory tract using a nebulizer every 30 minutes for 1 day, 3 times per week, for 3 weeks (1% OVA in normal saline for first 2 weeks and 2% OVA in normal saline for last 1 week) (Fig. 2).
- Seven days after the sensitization by intratracheal injection with OVA, the normal group was exposed to aerosolized PBS and the OVA control group was exposed to aerosolized OVA for 30 min/day, 3 days/week, for 3 weeks, in a polycarbonate chamber (40 ㎝ × 25 ㎝ × 27 ㎝). The OVA + CLEO (1% or 3%) groups were exposed to CLEO for 30 min/day, 3 days/week, for 3 weeks after OVA sensitization, in a polycarbonate chamber. Cyclosporin A (10 ㎎/㎏) was orally administered 3 times a week for the last 3 weeks as a positive control to the OVA-CsA group after OVA sensitization (Fig.
- The gas chromatography-mass spectrometry (GC-MS) analysis of CLEO was performed using an Agilent 6890N GC (Agilent Technologies, Santa Clara, CA, USA) interfaced with an Agilent 5975 (Agilent Technologies, Santa Clara, CA, USA) inert mass selective detector. The GC column was a HP-INOWAX (60 m × 0.
- During the 3-week treatment period, CLEO was inhaled and the final aerosolized 2% OVA solution was sprayed. Then, 24 h later, MCH (bronchoconstrictor) aqueous solution (3.125, 6.25, 12.5, and 25 ㎎/mL concentrations) was aerosolized and administered to 5 groups of mice, in which airway reactivity was observed for 15 minutes. Penh was calculated as follow :
- obtusa essential oil suggest its potential to mitigate asthma through inhibition of the inflammatory response. Therefore, in this study, we extracted the essential oils from the leaf of Chamaecyparis obtusa, indigenous to Korea, and analyzed the components of its essential oils (CLEO), and investigated immunologically the effects of CLEO against airway hyperresponsiveness (AHR) and airway inflammation (AI) by using asthma mouse model.
- AHR is a characteristic of asthma and indicates the degree of airflow limitation caused by hypersensitive reactions to stimuli that cause airway constriction (Kim, 2009). To measure AHR in this study, the Buxco system was used to administer varying concentrations of the airway constrictor methacholine (MCH) via spraying to each of the treatment groups, which was followed by measurement of the Penh value, which indicates the degree of airway constriction. A comparison of the measured values is shown in Fig.
대상 데이터
- C. obtusa leaves were collected in Yeongdong-gun Jagye-ri, Chungchoengbukdo, Korea and dried under shade at room temperature for 23 days after washing with distilled water. Essential oil was extracted using a simultaneous steam distillation and extraction (SDE) apparatus (Serkan and Gonca, 2009).
- Six-week-old female BALB mice (21 ± 1 g) were obtained from Daehan Biolink Co. of Seongnam, Korea.
- Among the constituents of CLEO, quantitative analysis of components that were commercially available as reference materials (such as α-pinene, limonene, bornyl acetate, and terpinene-4-ol) was performed. The reference materials were purchased from Sigma Aldrich Co. (St Louis, MO, USA). The GC-MS analysis conditions were identical to those of the qualitative analysis, and the validation method verified the precision, accuracy, and linearity of the calibration curve.
데이터처리
- Data are expressed as mean ± standard error of the mean (SEM). Statistical analysis of comparisons between groups was performed using the Student t-test. Significance was assessed as p < 0.
- Statistical analysis of data was analyzed using Student's t-test.
- Statistical analysis of data was analyzed using Student’s t-test.
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