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녹비작물 재배가 유기포도 수량 및 안토시아닌 함량에 미치는 영향
Effect of Green Manure Cultivations on Yield and Anthocyanin Content in Organic Grapevine 원문보기

韓國有機農業學會誌 = Korean journal of organic agriculture, v.23 no.2, 2015년, pp.281 - 289  

박준홍 (Gyeongsangbukdo Agricultural Research & Extension Services) ,  박상조 (Gyeongsangbukdo Agricultural Research & Extension Services) ,  권오흔 (Gyeongsangbukdo Agricultural Research & Extension Services) ,  최성용 (Gyeongsangbukdo Agricultural Research & Extension Services) ,  박소득 (Gyeongsangbukdo Agricultural Research & Extension Services)

Abstract AI-Helper 아이콘AI-Helper

In sustainable viticulture, green manure represents a safe and non-polluting way to bring large quantities of organic matter into the soil. The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. This study was conducted to evaluate th...

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제안 방법

  • Soil available phosphate was determined according to the method of Lancaster. For determination of soil exchangeable cations (K, Ca, and Mg), air-dried soil samples were extracted with 1 N ammonium acetate for 30 minutes, and concentration of the extract was determined by AAS (Analyst 400, Perkin Elmer, USA).
  • Chemical analysis for determination of the mineral content of each sample was conducted according to methods of soil chemical analysis (NIAST, 1988). For the determination of soil pH, air-dried soil samples were mixed with deionized water with a ratio of 1 : 5 (soil : water) and the pH of the clear supernatant was measured by pH electrode (Orion VERSA STAR, Thermo fisher scientific, US), and then, soil electric conductivity (EC) was measured by electric conductivity meter (CM-30R, DKK-TOA, Japan). Soil NO3-N content was measured by Kjeldahl apparatus (K-314, BUCHI, Switzerland), after extracting from fresh soil sample with 2 M KCl for 30 minutes.
  • The analysis of anthocyanins in grape skins was carried out using a HPLC system (Waters Alliance 2695, USA) equipped with a ACQUITY UPLC BEH C18 reverse phase column (2.1 × 50 mm).
  • (20 m × 10 m) and all experiments were conducted by a randomized complete block design with three replications. The experiments were treated with hairy vetch (Vicia villosa), rye (Secale cereale), mixture of hairy vetch and rye, and natural grass as control. Hairy vetch and rye were fall-seeded at rate of 90 kg and 200 kg ha-1, a mixture of hairy vetch and rye were drilled at the rate of 45 kg ha-1 and 100 kg ha-1, respectively.
  • The size of each experimental plot was 200 m2 (20 m × 10 m) and all experiments were conducted by a randomized complete block design with three replications.

대상 데이터

  • The experimental fields were located at 36°19ʹ40ʺ N latitude and 127°56ʹ51ʺE longitude.
  • This study was conducted on the organic farm field at sangju, Gyeongsangbuk-do, Korea, from October 2012 through September 2013. The experimental fields were located at 36°19ʹ40ʺ N latitude and 127°56ʹ51ʺE longitude.

데이터처리

  • The results of each parameter in all three applications were subjected to analysis of variance, and treatment means were compared by Duncan’s multiple range test (DMRT) at the 5% probability level.
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참고문헌 (14)

  1. Allison, L. E. 1965. Organic carbon. In Methods of soil analysis, ed. C. A. Black, Madison, Wisc., ASA. pp. 1367-1376. 

  2. Bongue-Bartelsman, M. and D. A. Phillips. 1995. Nitrogen stress regulates gene expression of enzymes in the flavonoid biosynthetic pathway of tomato. Plant Physiol. Biochem. 33: 539-546. 

  3. Clark, A. 2007. Managing cover crops profitably (third edition). Sustainable agriculture network. MD, USA. 

  4. Contoman, M. 2011. Researches concerning the improvement of some agrochemical and microbiological qualities of vine-planted soils under the influence of ecological fertilizer. J. of Environmental Protection and Ecology. 12(4A): 2132-2140. 

  5. Ebelhar, S. A., W. W. Frye and R. L. Blevins. 1984. Nitrogen from legume cover crops for no-tillage corn, Agron. J. 76: 51-55. 

  6. Haynes, R. J., R. J. Martin and K. M. Goh. 1993. Nitrogen fixation, accumulation of soil nitrogen, and nitrogen balance for some field grown legume crops, Field Crops Res. 35: 85-92. 

  7. Krisa, S., P. W. Teguo, A. Decendit, G. Deffieux, J. Vercauteren and J. M. Merillon. 1999. Production of 13C-labelled anthocyanins by Vitis vinifera cell suspension cultures. Phytochem. 51: 651-656. 

  8. Kakegawa, K., J. Suda, K. Sugiyama and A. Komamine. 1995. Regulation of anthocyanin biosysntesis in cell suspension cultures of Vitis in relation to cell division. Physiol. Plant. 94: 661-666. 

  9. NAAS. 2010. Fertilizer application recommendations for crop plants. National Academy of Agricultural Science, RDA, Suwon, Korea. 

  10. Power, J. F. and J. A. Zachariassen. 1993. Relative nitrogen utilization by legume cover crop species at three soil temperatures. Agron. J. 85: 134-140. 

  11. Roggero, J. P., S. Coen and B. Ragonnet. 1986. High performance liquid chromatography survey on changes in pigment content in ripening grapes of Syrah. An approach to anthocyanin metabolism. Am. J. Enol. Vitic. 37: 77-83. 

  12. Sato, K., M. Nakayama and J. Shigeta. 1996. Culturing conditions affecting the production of anthocyanin in suspended cell cultures of strawberry. Plant Sci. (Limerkck) 113: 91-98. 

  13. Tan, S. and G. D. Crabtree. 1990. Competition between perennial ryegrass sod and Chardonnay wine grapes for mineral nutrients. HortScience. 25(5): 533-535. 

  14. Utomo, M., W. W. Frye and R. L. Blevins. 1990. Sustaining soil nitrogen for corn using hairy vetch cover Crop. Agron. J. 82: 979-983. 

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