[논문]가미계작지모탕약침(加味桂芍知母湯藥鍼)이 퇴행성관절염 병리인자 및 동물병태 모델에 미치는 영향

이현재; 박정오; 오민석

doi:10.18325/jkmr.2015.25.4.1

가미계작지모탕약침(加味桂芍知母湯藥鍼)이 퇴행성관절염 병리인자 및 동물병태 모델에 미치는 영향
Anti-inflammation and Anti-oxidation Effects of Gamikyejakjimo-tang Herbal Acupuncture on Pathologic Factor and MIA-induced Osteoarthritis Rat 원문보기

Journal of Korean Medicine Rehabilitation : JKMR = 한방재활의학과학회지, v.25 no.4, 2015년, pp.1 - 20

이현재 (대전대학교 한의과대학 한방재활의학과교실) , 박정오 (대전대학교 한의과대학 한방재활의학과교실) , 오민석 (대전대학교 한의과대학 한방재활의학과교실)

Abstract ▼ AI-Helper

Objectives This study was intended to clarify the anti-inflammation and anti-oxidation effects of gamikyejakjimo-tang herbal acupuncture (GKHA) for osteoarthritis. Methods Osteoarthritis was induced by injection of MIA into right knee joint cavities of rats. Rats were divided into a total of 4 groups (n=8). The 4 groups were normal group, control group, positive comparison group and expeimental group. Indomethacin and GKHA were medicated for a total of 4 weeks. After that, functions of liver and kidney by AST, ALT, creatinine, BUN, DPPH and ABTS free radical scavenging activity, ROS (reactive oxygen species) production, NO (Total Nitric oxide), IL-$1{\beta}$, IL-6, TNF-${\alpha}$ production, weight changes in the hind legs of MIA-induced osteoarthritis rat, serum PGE2, TIMP-1, MMP-2, MMP-9, LTB4, hs-CRP, and white blood cells, neutrophils, lymphocytes, monocytes were measured. The volume of cartilage was observed by micro CT arthrography. H&E and Safranin-O staining were used to examine the injury of synovial tissue. Results 1. In the hind leg weight bearing measurement, level of weight was increased. 2. AST, ALT, BUN, creatinine were decreased. 3. The production of total white blood cell was decreased, and the production of neutrophils, lymphocytes, monocytes were significantly decreased. 4. The production of NO, PGE2, TIMP-1, MMP-2, MMP-9, LTB4 were significantly decreased, and the production of hs-CRP was also decreased but with no significance. 5. The cartilage volume was significantly increased. 6. In H&E staining and Safranin-O staning, the cartilage cell appeared to be proliferated, and proteoglycans appeared to be increased. Conclusions Based on the results above, Gamikyejakjimo-tang Herbal Acupuncture has anti-oxidation and anti-inflammation effects, which leads to suppressing the underlying causes and the progression of osteoarthritis.

주제어

AI 본문요약
AI-Helper

문제 정의

이에 저자는 桂芍知母湯이 관절 통증에 유효하다는 문헌적 근거 및 기존 연구에 착안하여, 본 연구에서는 桂芍知母湯에 牛膝, 威靈仙을 加한 加味桂芍知母湯藥鍼液 (GKHA)을 이용하여 골관절염에 미치는 치료효과를 규명 하고자 하였다. RAW 264.

제안 방법

이에 저자는, 桂芍知母湯의 골관절염에 대한 효과를 더욱 증진하고자 散瘀血, 消癰腫, 强筋骨하는 牛膝²⁵⁾과 祛風除濕, 通絡止痛하는 威靈仙²⁵⁾을 加味하여 加味桂芍知母湯藥鍼(GKHA) 제조한 후, LPS로 자극된 RAW 264.7 cell에서 염증 유발 인자를 측정하고, MIA로 유발된 rat의 혈액 내 면역세포, 뒷다리 체중부하, cartilage volume 및 조직병리학적 검사 등을 실시한 결과 유의한 성적을 얻었기에 보고하는 바이다.

대상 데이터

본 실험에 사용한 加味桂芍知母湯(Gamikyejakjimo-tang 이하, GK로 표기)의 구성 약재들은 ㈜옴니허브(Daegu, Korea)에서 구입하였고, 대전대학교 traditional and biomedical research center-regional innovation center (TBRC-RIC)에서 확인 후 정선해서 사용하였다. ｢金匱要略｣²²⁾을 바탕으로 용량을 가감하였으며 그 내용과 분량(1 첩)은 다음과 같다(Table I).
수컷 6 주령의 SD-Rat (170∼200 g)을 ㈜라온바이오사(Yongin, Korea)에서 공급받아 실험하였다. 동물은 실험 당일까지 일반 고형사료를 ㈜퓨리나사료(Seongnam, Korea)에서 공급받아 충분히 투여하여 온도 22±2℃, 습도 55±15%, 12시간(light-dark cycle)의 환경에서 1주간 적응시킨 후 실험에 사용하였다.
실험에 사용된 RAW 264.7 세포는 한국세포주은행 (Seoul, Korea)에서 구입하였다.

데이터처리

실험 결과는 SPSS 11.0의 unpaired student’s T-test를 사용하여 통계처리 하였으며 p＜0.05, p＜0.01 및 p ＜0.001 수준에서 유의성을 검정하였다.

성능/효과

RAW 264.7 세포에서 항염증 효능을 확인하기 위해 IL-1β, IL-6 및 TNF-α를 측정한 결과, IL-1β 항목에서는 GK 100 μg/ml 농도에서 유의성 있는 감소를 나타내었으며(Fig. 5), IL-6 및 TNF-α 항목에서는 1, 10, 100 μg/ml 농도에서 각각 모두 유의성 있는 감소를 나타내었으며, 이는 GK가 골관절염의 염증 억제에 효과가 있음을 나타낸다.
이상의 결과로 보아 加味桂芍知母湯藥鍼은 항산화, 항염증 및 진통 효과가 있고, MIA로 유도된 rat의 골관절염에서 염증 cytokine 및 골관절염 진행 인자를 효과적으로 억제하며, 연골파괴를 감소시켜 골관절염의 치료 뿐만 아니라 예방에도 유효하리라 생각된다.
혈청 내 염증 사이토카인 및 인자에 미치는 영향을 확인하고자 NO, PGE2, TIMP-1, MMP-2, MMP-9, LTB4, hs-CRP를 측정한 결과, MMP-2를 제외한 각각의 인자에서 GKHA 투여군이 대조군에 비해 모두 유의성 있는 감소를 보였다. PGE2 및 LTB4 감소는 GKHA 투여로 콜라겐 생산 및 골대사를 증가시켜 염증 반응을 억제시켰을 것으로 사료되며, TIMP-1 감소는 GKHA 투여군에서 기질의 이화작용을 감소시키고 관절 결체 조직의 변성을 억제한 것으로 보인다.

질의응답

핵심어	질문	논문에서 추출한 답변
	골관절염이란?	골관절염은 퇴행성 관절염 또는 퇴행성 관절질환이라고도 불리며, 국소 관절에 점진적인 관절연골의 소실 및 그와 관련된 2차적인 변화와 증상을 동반하는 질환이다1).
	2012년 국민건강영양조사통계에 의하면, 연령별 골관절염 유병률은 어떠한가?	2012년 국민건강영양조사통계4)에 의하면 연령별 골관절염 유병률은 남자 50대에서 4.5%, 60대 5.5%, 70대 20.0%였고, 여자는 50대 15.4%, 60대 32.8%, 70대 65.2%로 남녀 모두 연령이 높아질수록 유병률이 급격히 증가하고 있으며, 향후 노령화 시대와 더불어 골관절염의 유병률은 더욱 더 증가할 것으로 예상된다.
	골관절염의 특징은?	주로 관절 연골의 국소적 퇴행성 변화, 연골하골의 비대, 주변 골연골부의 골형성, 관절의 변형을 특징으로 하며2), 예전에는 가장 중요한 원인이 노령화에 따른 단순 퇴행성 변화라고 생각했지만 최근에는 여러 연구로 인해 수많은 요인들이 관여하는 것으로 인식되고 있다3).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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