[논문]통비산(通痺散) 열수추출물의 항염증반응 및 항산화활성에 대한 연구

김용민; 김희택; 김이화

doi:10.6114/jkood.2016.29.4.024

통비산(通痺散) 열수추출물의 항염증반응 및 항산화활성에 대한 연구
Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.29 no.4, 2016년, pp.24 - 33

김용민 (세명대학교 한방바이오융합과학부) , 김희택 (세명대학교 한의과대학 한방안이비인후피부과학과) , 김이화 (세명대학교 한의과대학 경락경혈학교실)

Abstract ▼ AI-Helper

Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin $E_2$ ($PGE_2$) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability. Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of $PGE_2$ was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method. Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

The purpose of this study is to investigate the effects of TS on the production of NO and PGE₂ induced by LPS-stimulated RAW 264.7 cells and DPPH free radical scavenging capability.

가설 설정

2. TS did not increased significantly compared to the untreated group in the cell cytotoxicity.
4. TS had the DPPH free radical scavenging capability.

제안 방법

1. This study is to investigate the antiinflammatory and anti-oxidant effects of TS extract on RAW264.7 macrophages using by cell cytotoxicity, NO and PGE₂ production and DPPH free radical scavenging capability.
In order to find out the concentration at which the cytotoxic effect of TS on the RAW 264.7 cell line become evident, cells were cultured with TS at final concentrations of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖, and 400 ㎍/㎖ for 24 hrs, and MTT assays were carried out, with cells cultured in TS-free media as the control. The viabilities of cells incubated with TS at concentrations of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖ and 400 ㎍/㎖ were 99.
In this study, we used TS as the potent NO and PGE₂ production inhibitor to investigate the involvement of anti-inflammation and anti-oxidant effect. The TS reduced NO production and PGE₂ production induced by the induction of LPS, and increased the radical scavenging activity.

대상 데이터

Cells of the murine macrophage RAW 264.7 were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea). Cells were cultrued in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco BRL, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco BRL) at 37℃ in 5% CO₂ , 95% O₂ in a humidified cell incubator.

데이터처리

Statistical analysis was performed using Student's t-test (SPSS ver 12.0) and the results were expressed as mean ± S.E.M. Differences were considered significant for p < 0.05.

이론/모형

The control group was not treated with LPS or TS. Supernatant from cell culture media was collected, and NO levels were determined with the Griess assay. TS was found to inhibit LPS-induced NO productions in a dose-dependent manner.

성능/효과

The NO production of TS at concentrations of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖ and 400 ㎍/㎖ were 98.46 ± 3.35 %, 93.85 ± 7.02 %, 90.49 ± 2.14 % and 78.01 ± 3.95 % of the control (100 ± 4.87 %) value respectively.
The viabilities of cells incubated with TS at concentrations of 50 ㎍/㎖, 100 ㎍/㎖, 200 ㎍/㎖ and 400 ㎍/㎖ were 99.41 ± 1.60 %, 97.47 ± 1.87 %, 94.39 ± 2.88 % and 88.50 ± 5.71 % of the control (100 ± 1.63 %) value respectively.

참고문헌 (35)

Cordell GA. Phytochemistry and traditional medicine-the revolution continues. Phytochem Lett. 2014;10:28-40.

상세보기
World Health Organization. WHO Traditional medicine strategy. 2013. Availble from:URL: http://www.who.int/medicines/publications/traditional/trm-strategy14_23.
Torres-Rodrigues ML, Garcia-Chavez E, Berhow M, de Mejia EG. Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages. J Ethnopharmacol. 2016;188:266-74.

상세보기
Tracey KJ. The inflammatory reflex. Nature. 2002;420:853-9.

상세보기
Chen X, Miao J, Wang H, Zhao F, Hu J, Gao P, et al. The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF- ${\kappa}B$ pathway. J Ethnopharmacol. 2015;170:72-80.

상세보기
Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. Journal of the Association of Physicians of India. 2004;52:794-804.

상세보기
Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem. Toxicol. 2002;40:1745-50.

상세보기
Kim HK, Cheon BS, Kim YH, Kim SY, Kim HP. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem. Pharmacol. 1999;58:759-65.

상세보기
Surh YJ, Chun KS, Cha HH, Han SS, Keum Y, Park K, et al. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: downregulation of COX-2 and iNOs through suppression of $NF{\kappa}B$ activation. Mutat Res. 2001;480-481:243-68.

상세보기
Chang YC, Li PC, Chen BC, Chang MS, Wang JL, Chiu WT, et al. Lipoteichoic acid-induced nitric oxide synthase expression in RAW 264.7 macrophages is mediated by cyclooxygenase-2, prostaglandin $E_2$ , protein kinase A, p38 MAPK, and nuclear factor-kappa B pathways. Cell Signal. 2006;18:1235-43.

상세보기
Zhang L. Zhang's Treatise on general medicine. 2nd ed. Beijing:Traditional Chinese Medical Publishing Company in China. 1995;809-10.
Lim HH, Kim YO, Seo MJ, Choi SW. Anti-Inflammatory Effects of Volatile Flavor Extract from Herbal Medicinal Prescriptions Including Cnidium officinale Makino and Angelica gigas Nakai. Journal of the society of cosmetics scientists of korea. 2011;37:199-210.
Han KS, Kim KC, Wang JH, Kim HJ. Effect of Unfermented and Fermented Atractylodes macrocephalae on Gut Permeability and Lipopolysaccharide-Induced Inflammation. Journal of Society of Korean Medicine for Obesity Research. 2013;13:24-32.
Cho JH, Kwon JE, Cho Y, Kim I, Kang SC. Anti-inflammatory effect of Angelica gigas via Heme Oxygenase-1 expression. Nutrients. 2015;15:4862-74.
Chen LG, Jan YS, Tsai PW, Norimoto H, Michihara S, Murayama C, et al. Antiinflammatory and Antinociceptive constituents of Atractylodes joponica koidzumi. J Agric Food Chem. 2016;64:2254-62.

상세보기
Xu L, Kitade H, Ni Y, Ota T. Roles of chemokines and chemokine receptors in obesity-associated insulin resistance and nonalcoholic fatty liver disease. Biomolecules. 2015;5:1563-79.

상세보기
Tilg H, Moschen AR. Inflammatory mechanisms in the regulation of insulin resistance. Mol Med. 2008;14:222-31.

상세보기
Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860-7.

상세보기
Reed GA, Arneson DW, Putnam WC, Smith HJ, Gray JC, Sullivan DK, et al. Single-dose and multiple-dose administration of indole-3-carbinol to women: pharmacokinetics based on 3,3'-diindolymethane. Cancer Epidermiol. Biomarkers Prev. 2006;15:2477-81.

상세보기
Xia Z, Triffitt JT. A review on macrophage response to biomaterials. Biomed. Mater. 2006;1:1-9.

상세보기
Ross JA, Auger MJ, Burke B, Lewis CE. The biology of the macrophage. In:Burker B, Lewis CE(Eds.). The Macrophage. 2nd ed. Oxford:Oxford Medical Publications. 2002:1-72.
Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, et al. Defective LPS signaling in C3H/Hej and C57BL/10ScCr mice. Mutations in Tlr4 gene. Science. 1998;282;2085-8.

상세보기
Schmidt HH, Walter U. NO at work. Cell. 1994;78:919-25.

상세보기
St Clair EW, Wilkinson WE, Lang T, Sanders L, Misukonis MA, Gilkeson GS, et al. Increased expression of blood mononuclear cell nitric oxide synthase type 2 in rheumatoid arthritis patients. Journal of Experimental Medicine. 1996;184:1173-8.

상세보기
Worrall NK, Lazenby WD, Misko TP, Lin TS, Rodi CP, Manning PT, et al. Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase. Journal of Experimental Medicine. 1995;181:63-70.

상세보기
Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sciences. 2001;69:625-35.

상세보기
Hu XD, Yang Y, Zhong XG, Zhang XH, Zhang YN, Zheng ZP, et al. Antiinflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages. Journal of Ethnopharmacology. 2008;120:447-51.

상세보기
Bak MJ, Truong BL, Kang HS, Jun M, Jeong WS. Anti-inflammatory effect of procyanidins from wild grape (Vitis amurensis) seeds in LPS-induced RAW264.7 cells. Oxid Med Cell Longev. 2013:36;340-8.
Parthasarathy S, Khan-Marchant N, Penumeteha M, Santanam N. Oxidative stress in cardiovascular disease. J Nuclear Cardiol. 2001;8(3):379-89.

상세보기
Benedetti S, Benvenah F, Pagharami S, Francegh S, Stephano S, Canestrari F. Antioxidant properties of a novel phycocyania extract from the blue-green alga Aphanizomenonflos-aquae. Life sci. 2004;75:2353-62.

상세보기
Guerci B, Bohme P, Kearney-Schwartz A, Zannad F, Drouin P. Endothelial dysfunction and type 2 diabetes. Diabetes Metab. 2001;27:436-47.
Pincemail J, Meurisse M, Limet R, Defraigne JO. L'evaluation du stress oxydatif d'un individu : une realite pour le medecin. Vaisseaux Coeur Poumons. 1999;4(5):12-23.
Drobek-Slowik M, Karezewicz DK. The potential role of oxidative stress in the pathogenesis of the age-related macular degeneration (AMD). Postepy Hig Med Dosw. 2007;61:28-37.
Zafra-Stones S, Yasmin T, Bagchi M, Chattejee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prvention. Mol Nutr Food Res. 2007;51:675-83.

상세보기
Tang Q, Chen L, Jie Y. Effects of traditional Chinese medicine Drynaria fortuneismith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells. China Journal of Chinese Mareria Medica. 2004;29(2):64-168.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증