[논문]골쇄보 열수추출물 약침액의 항염증반응 및 DPPH 라디칼 소거능 활성에 대한 연구

김용민; 김이화

doi:10.14406/acu.2015.026

골쇄보 열수추출물 약침액의 항염증반응 및 DPPH 라디칼 소거능 활성에 대한 연구
The Anti-inflammatory Effect and DPPH Free Radical Scavenging Capability of Rhizoma drynariae Aqueous Extract 원문보기

Korean journal of acupuncture, v.32 no.4, 2015년, pp.169 - 176

김용민 (세명대학교 한방화장품과학과) , 김이화 (세명대학교 한의과대학 경락경혈학교실)

초록
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목적 : 한의학에서 골절이나 신장질환에 사용되어 왔던 골쇄보열수추출물 약침액이 항염증 및 항산화활성에 대한 효능이 있는지를 연구하고자 한다. 방법 : 골쇄보열수추출물 약침액이 세포독성에 미치는 영향을 관찰하기 위하여 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay를 실시하였다. 골쇄보열수추출물 약침액이 항염증효능에 미치는 영향을 관찰하기 위하여 lipopolysaccharide(LPS)로 유도된 RAW264.7 대식세포에서의 산화질소(NO) 및 프로스타글란딘($PGE_2$) 생성 억제력을 관찰하였다. 또한 골쇄보열수추출물 약침액이 항산화활성에 미치는 영향을 관찰하기 위하여 1,1-diphenyl-2-pciry hydrazyl(DPPH) radical 소거능을 관찰하였다. 결과 : 골쇄보열수추출물 약침액은 $50{\sim}400{\mu}g/ml$ 농도에서 세포독성이 나타나지 않았다. 골쇄보열수추출물 약침액은 200 및 $400{\mu}g/ml$ 농도에서 LPS로 유도된 NO 및 $PGE_2$ 생성을 통계학적으로 유의하게 감소시켰다. 골쇄보추출물은 $50{\sim}400{\mu}g/ml$ 농도에서 DPPH 소거능을 관찰한 결과 농도의존적으로 활성화되는 것을 관찰하였다. 결론 : 골쇄보열수추출물 약침액은 항염증 및 항산화 효과가 있을 것으로 사료된다.

Abstract ▼ AI-Helper

Objectives : This study is to investigate the effects of Rhizoma drynariae aqueous extract(RDA) on cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin $E_2(PGE_2)$ production and 1,1-diphenyl-2-picryl ghdrazyl(DPPH) free radical scavenging capability. Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The production of NO was measured by Griess assay. The production of $PGE_2$ was measured by immunoassay. And, the anti-oxidant activity was measured by the DPPH method. Results : Cell cytotoxicity in 50, 100, 200 and $400{\mu}g/ml$ RDA did not increase significantly compared to the RDA untreated group. RDA($200{\mu}g/ml$ and $400{\mu}g/ml$) inhibited NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells. RDA had high DPPH free radical scavenging capability. Conclusions : This study indicates that RDA inhibits NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells and improve DPPH free radical scavenging capability. RDA may have an anti-inflammation effect and an anti-oxidant activity.

주제어

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문제 정의

The purpose of this study is to investigate the effects of RDA on the production of NO and PGE₂ induced by LPS-stimulated RAW 264.7 cells and DPPH free radical scavenging capability.

제안 방법

In order to find out the concentration at which the cytotoxic effect of RDA on the RAW 264.7 cell line become evident, cells were cultured with RDA at final concentrations of 50 μg/ml, 100 μg/ml, 200 μg/ml, and 400 μg/ml for 24 hrs, and MTT assays were carried out, with cells cultured in RDA-free media as the control.
drynariae, the mechanism of the anti-inflammatory responses and anti-oxidant effects has not been elucidated. In this study, we investigated the anti-inflammatory and the anti-oxidant effects of R. drynariae using by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay, nitric oxide(NO) and prostaglandin E₂(PGE₂) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) assay.

대상 데이터

Cells of the murine macrophage RAW 264.7 were purchased from the Korean Cell Line Bank(KCLB, Seoul, Korea). Cells were cultured in Dulbecco’s Modified Eagle Medium(DMEM)(Gibco BRL, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum(FBS) (Gibco BRL) at 37℃ in 5% CO₂, 95% O₂ in a humidified cell incubator.

데이터처리

Statistical analysis was performed using Student’s t-test (SPSS 11.0 software) and the results were expressed as mean±S.E.M. Differences were considered significant for p＜0.05.

이론/모형

The control group was not treated with LPS or RDA. Supernatant from cell culture media was collected, and NO levels were determined with the Griess assay. RDA was found to inhibit LPS-induced NO productions in a dose-dependent manner.

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