[논문]추출용매에 따른 단삼 추출물의 항노화 활성

곽남; 이지안

doi:10.22156/cs4smb.2022.12.05.290

추출용매에 따른 단삼 추출물의 항노화 활성
Effects of solvents on the anti-aging activity of Salvia miltiorrhiza extract 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.12 no.5, 2022년, pp.290 - 297

곽남 (서경대학교 일반대학원 미용예술학과) , 이지안 (서경대학교 미용예술대학 뷰티테라피&메이크업학과)

초록
AI-Helper

본 연구에서는 단삼 뿌리 추출물의 세포 안정성, 수렴 활성, NO 소거능, iNOS 단백질 발현량, 염증성 cytokine 분비, elastase 효소 저해능, type I pro-collagen 합성능 등을 조사하여 기능성 화장품 소재로서의 가능성을 평가하고자 하였다. 단삼 80% 에탄올추출물(SE)과 열수추출물(SW)을 대상으로 NHDF 및 RAW264.7 세포에서 0.05~0.5 mg/mL 농도 처리에 따른 독성을 보이지 않아 수렴, 염증, 주름 개선 효능을 진행하였다. 그 결과 수렴활성 측정 결과 SE는 10 mg/mL에서 74.6%의 활성을 나타냈다. SE는 농도 의존적으로 LPS에 의해 유도된 NO, iNOS 단백질, TNF-α 및 IL-6 cytokine의 생성을 유의미하게 감소시켰다. 게다가 SE와 SW는 elastase 효소 활성을 억제할 뿐만 아니라 TNF-α에 의해 감소된 pro-collagen의 생성을 증가시켰다. 따라서 단삼 에탄올추출물은 수렴제, 염증 관련 피부 질환, 주름 생성을 효과적으로 예방할 수 있는 천연 화장품 소재로서의 활용 가능성이 높을 것으로 기대된다.

Abstract ▼ AI-Helper

This study was conducted to determine the cell cytotoxicity, astringency, nitrite oxide scavenging, iNOS protein expression level, pro-inflammatory cytokine, elastase inhibition, and type I pro-collagen synthesis as a functional cosmetics material of Salvia miltiorrhiza root. We prepared the 80% ethanol(SE) and hot-distilled water(SW), respectively. Both SE and SW showed no toxicity from 0.05 to 0.5 mg/mL concentration as a result of MTT assay in NHDF or RAW264.7 cells. In the measurement of astringent effect, SE reveled 74.6% of astringent activity in 10 mg/mL. SE showed that LPS-induced nitric oxide production, iNOS protein expression, and cytokines were inhibited in a dose-dependent manner. Furthermore, two extracts significantly inhibited elastase activity and increased the type I pro-collagen production. Therefore, it is expected that Salvia miltiorrhiza extract is used as a natural material for functional cosmetics that can effectively prevent skin-related inflammation and wrinkles, and aging.

주제어

표/그림 (8)

표 Table 1. Extraction yield of S. miltiorrhiza by 80% ethanol and hot-water
그림 Fig. 1. Effect of S. miltiorrhiza on the cell viability: (A) NHDF and (B) RAW264.7 cells. Values are expressed as mean±S.D.
그림 Fig. 2. Effect of astringent activity of two extracts from S. miltiorrhiza. Values are expressed as mean±S.D. Statistically significant from the control. (*p<0.05).
그림 Fig. 3. Effect of nitric oxide production of two extracts from S. miltiorrhiza. Values are expressed as mean±S.D. Statistically significant from the control. (*p<0.05, **p<0.01).
그림 Fig. 4. Effect of iNOS protein expression of two extracts from S. miltiorrhiza. Values are expressed as mean±S.D.
그림 Fig. 5. Effect of pro-inflammatory cytokines of two extracts from S. miltiorrhiza. :(A) TNF-α and (B) IL-6. in LPS-induced RAW264.7 cells. Values are expressed as mean±S.D. Statistically significant from the control. (*p<0.05, **p<0.01).
그림 Fig. 6. Inhibitory effect of elastase activity of two extracts from S. miltiorrhiza. Values are expressed as mean±SD. Statistically significant from the control. (*p < 0.05, **p<0.01).
그림 Fig. 7. Type I pro-collagen synthesis rate in NHDF cells. Values are expressed as mean±S.D. Statistically significant from the control. (*p<0.05).

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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