[논문]Investigation of Antioxidative Activity from Apple (Malus pumila Miller) Peel by Various Extraction Solvents

Kim, Gyeong-Hwuii; Duan, Yishan; Gwon, Soon-Hee; Kim, Han-Soo

doi:10.12925/jkocs.2016.33.1.186

Investigation of Antioxidative Activity from Apple (Malus pumila Miller) Peel by Various Extraction Solvents 원문보기

한국유화학회지 = Journal of oil & applied science, v.33 no.1, 2016년, pp.186 - 194

Kim, Gyeong-Hwuii (Department of Biological Science and Technology, Yonsei University) , Duan, Yishan (Department of Food Science and Technology, Pusan National University) , Gwon, Soon-Hee (College of Nano Science and Technology, Office of Administration, Pusan National University) , Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)

Abstract ▼ AI-Helper

Consumption of fruits and vegetables has been conducted to be effective in the prevention of chronic diseases. In this study, 70% methanol, 70% ethanol and chloroform-methanol mixture (CM, 2:1, v/v) were used as solvents in the extraction of apple peels. The total phenol content, total flavonoid content and antioxidant activity of various extracts were investigated using in vitro assays. The extract obtained by 70% methanol showed the highest total phenol content ($20.87{\pm}0.17mgCAE/g$), ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity and ferric reducing antioxidant power. However, 70% ethanol extract possessed the strongest antioxidant activity assayed by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and ${\beta}$-carotene bleaching method. And CM extract was found to show the highest total flavonoid content with the value of $9.26{\pm}0.06mgQE/g$. These results indicated that apple peels can be used in dietary applications with a potential to reduce oxidative stress.

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제안 방법

In this study, we investigated the total phenol contents, total flavonoid contents and antioxidant activity of 70% methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts from apple (Malus pumila Miller) peels. The employed in vitro antioxidant activity assays were consisted of DPPH radical, ABTS radical scavenging activity, ferric reducing antioxidant power and β-carotene bleaching assays.

대상 데이터

Apples (Malus pumila Miller) were purchased from Uiseong (Gyeongbuk, Korea). Apples were washed and peeled.

데이터처리

The experimental data in triplicate were subjected to analysis of variance (ANOVA) and expressed as mean±SD (n=3). ANOVA was performed by using the one-way analysis of variance procedures. Duncan’s multiple-range test was used to analysis the significant difference of means, and p<0.

이론/모형

Antioxidant activity of various extracts from apple (Malus pumila Miller) peels by using β-carotene bleaching method.
DPPH radical scavenging activity was measured according to the method of Blois and Duan et al [11,12]. Samples (2.
The antioxidant activity of different extract was evaluated according to the β-carotene bleaching method following the method of Elzaawely et al [14].
Total phenol contents were determined by according to the colorimetric Folin–Denis’ method [6,9] with caffeic acid as a stand compound (y=0.1141x+0.0076, R2=0.9941).

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