선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
성능/효과
- The effect of various pH conditions (4.5, 5.5, 6.0, and 6.5) was evaluated in the recombinant strain for the GABA production, under the standard temperature of 30℃. The result shows that at pH 6.
- 5) was evaluated in the recombinant strain for the GABA production, under the standard temperature of 30℃. The result shows that at pH 6.0 the recombinant strain can produce a maximum level of 0.64 g/l GABA from 10 g/l of glucose (Fig. 3B).
- Among the tested recombinant strains, recombinant strains with the scaffold complex produced higher GABA than recombinant strain without the scaffold complex. There was no significant variation in the GABA concentration, which suggests that the GABA production was no longer affected by the ratio of enzymes in the scaffolds (Fig. 3C).
- GABA production can be affected by various culture conditions, such as temperature, pH, scaffold complex, and glucose. Therefore, optimization of the GABA production conditions is considered as an essential step for the efficient production of GABA. Temperature is an important condition for the GABA production by maintaining the GABA producing enzymes at optimum condition.
- 63 g/l of GABA was produced. These data showed that 30℃ was the suitable temperature for the effective production of GABA (Fig. 3A).
- The final GABA concentration is an important factor as well as GABA yield. This result suggested a higher GABA concentration was obtained by using 20 g/l of glucose without the reduction of GABA production. Thus, the higher GABA concentration was obtained from 20 g/l of glucose.
- 36 g/l of GABA was produced from 10 g/l of glucose by overexpression of GabD, GabT, and GadC without coexpression of the scaffold architecture. When the enzymes were overexpressed along with the scaffold architecture at the ratio of 1:1:1, the GABA concentration was elevated to 0.71g/ l, which was 1.97-fold greater than GABA synthesized without the scaffold architecture. This result implies that the co-localization of enzymes can direct the flux from the TCA cycle to the GABA shunt pathway.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.