[논문]Anti-inflammatory Effect of Methanol Extract from Safflower Seeds

Kim, Hyun-Jin

doi:10.11620/ijob.2016.41.2.083

Anti-inflammatory Effect of Methanol Extract from Safflower Seeds 원문보기

International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, v.41 no.2, 2016년, pp.83 - 88

Kim, Hyun-Jin (Institute of Biomaterial.Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University)

Abstract ▼ AI-Helper

Periodontitis is an inflammatory disease, which destroys the connective tissue and the alveolar bone. Recently, it has been suggested that the effect of natural substances could be induced into an anti-inflammatory environment. However, the effect of Safflower seed extract (SAF-M) associated with periodontitis has not been investigated yet. Therefore, the purpose of this study was to assess the anti-inflammatory effects of SAF-M. Cytotoxicity was assessed through MTS analysis using hGF and hPDL cells. Periodontitis was induced by injecting LPS into gingival tissue on the maxillary molars of rats ($45{\mu}g$ LPS/one time, 3 times a week for 3 weeks). SAF-M was administered daily at 30 mg/kg and 100 mg/kg. Alveolar bone resorption was evaluated through the micro-CT. hGF and hPDL cells showed differential cytotoxicity in response to SAF-M at 5 mg/ml and 1 mg/ml concentrations. Micro-CT showed reduction of the alveolar bone resorption in the SAF-M treatment group. These results suggested that SAF-M is a potential therapeutic agent for periodontitis.

주제어

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문제 정의

However, in terms of the periodontitis treatment, it is not clear as to how it could be a help. Therefore, the purpose of this study is to assess the effect of SAF-M in periodontitis induced by LPS in the rat models.
However, the report about the role of SAF-M on the alveolar bone loss in periodontitis has not been studied. Therefore, this study was performed to evaluate the effect of the SAF-M on the alveolar bone loss in periodontitis induced by LPS.

제안 방법

The alveolar bone and teeth were analyzed three dimensionally by using the Micro-CT (SkyScan 1172 Desktop MicroCT, Skyscan, Aartselaar, Belgium). The 3D digital image for each specimen was generated.
coil LPS 3 mg except for the control group. This procedure was repeated three times a week (Monday, Wednesday, and Friday) for three weeks. By injecting the same amount of 1 x PBS 15 ul, periodontitis was not induced in the negative control.
To evaluate the effect of SAF-M in hPDL and hGF cells, MTS assay was performed. Various concentrations of SAF-M were used to determine whether the cytotoxic effect of SAF-M on hGF and hPDL cells was occurred.

성능/효과

It was not known to be effective in anti-inflammatory activity SAF-M, but was found in this experiment. In conclusion, these results demonstrated that SAF-M suppressed the loss of alveolar bone induced by LPS. The regulatory mechanism of SAF-M related with lots of cytokines has not been revealed yet.

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Anti-inflammatory Effect of Methanol Extract from Safflower Seeds 원문보기

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Anti-inflammatory Effect of Methanol Extract from Safflower Seeds 원문보기

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