[논문]연교(連翹) n-BuOH 분획물의 아토피 피부염 억제 효과

이진화; 한재경; 김윤희

doi:10.7778/jpkm.2016.30.3.001

연교(連翹) n-BuOH 분획물의 아토피 피부염 억제 효과
The Effects of Forsythiae Fructus n-BuOH Fraction on Atopic Dermatitis 원문보기

大韓韓方小兒科學會誌 = The journal of pediatrics of Korean medicine, v.30 no.3, 2016년, pp.1 - 30

이진화 (대전대학교 한의과대학 소아과학교실) , 한재경 (대전대학교 한의과대학 소아과학교실) , 김윤희 (대전대학교 한의과대학 소아과학교실)

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Objectives Previous studies have found out that Forsythiae Fructus (FF) extracts have anti-atopic activities by in vitro experiment. In order to understand more about FF extracts' benefit, we subdivided FF extracts depending on systematic fractionation method by using Methylene chloride (MC), Ethyl acetate (EtOAc), n-BuOH and n-hexane (n-Hx). This study is designed to examine the effect of FF fractions on the PMA- ionomycin-induced activation of RBL-2H3 mast cell lines in vitro and on the DNCB-induced activation of NC/Nga mice in vivo. Methods For this study, we examined IL-4, IL-13 production by ELISA analysis, IL-4, IL-13, IL-31, IL-31RA and TNF-${\alpha}$ mRNA expression by real-time PCR and manifestations of AP-1 and MAPKs transcription factors by western blotting in vitro. Through in vitro experiment, we selected FF n-BuOH fraction that seems the best effective in atopic dermatitis then induced it on NC/Nga mice by DNCB. We measured mice's WBC, eosinophil and neutrophil in heart blood, IL-4, IL-5, IFN-${\gamma}$ in the spleenocyte culture supernatant, the absolute cell numbers of CD4+, CD8+, B220+CD23+, CD3+CD69+ and Gr-1+CD11b+ in the PBMCs, ALN and dorsal skin, IL-5, IL-13, IL-31, IL-31RA in the dorsal skin by real-time PCR and the distribution of immune cells by H&E on dorsal skin and ANL and toluidine blue staining on dorsal skin. Results FF n-BuOH fraction suppressed IL-4, IL-13 production and mRNA expression of IL-4, IL-13, IL-31, IL-31RA and TNF-${\alpha}$. Results from the western blot analysis showed that FF n-BuOH fraction reduced the activation of the mast cell specific transduction factors involved in AP-1 by suppressing JNK and ERK phosphorylation. In the gross, atopic dermatitis induced by DNCB in NC/Nga mice were improved by oral administration of FF n-BuOH fraction. Oral FF n-BuOH fraction also decreased the level of IgE in mice's serum and the level of IL-4 and IL-5 in the spleenocyte culture supernatant, cell numbers of CD8+, B220+CD23+ in the PBMCs, CD4+ in the ALN and CD4+, Gr-1+CD11b+ in the dorsal skin and suppressed mRNA expression of IL-5, IL-13, IL-31, IL-31RA in the dorsal skin. Histological examination showed that infiltration levels of immune cells in atopic dermatitis induced NC/Nga mice were improved by FF n-BuOH fraction. Conclusions FF n-BuOH fraction can reduce pruritus by suppressing IL-31, IL-31RA secretion and modulate molecular mediators and immune cells associated with atopic dermatitis induced in NC/Nga mice which may have played a significant role in recovering atopic dermatitis symptoms.

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핵심어	질문	논문에서 추출한 답변
	아토피 피부염이란?	아토피 피부염은 유 · 소아에 흔히 발생하는 소양감과 전형적인 피부염증 반응을 주증상으로 하는 만성 재발성 피부 염증질환이다1). 아토피 피부염의 원인은 아직 정확하게 밝혀지지 않았으나2) IgE 합성과 호산구 증가와 함께 T세포 면역반응에서 Th1 반응에 비해 Th2 반응이 우세하여 발생된 불균형이 핵심적인 면역학적 원인으로 보고되고 있다3-4).
	아토피 피부염의 원인은?	아토피 피부염은 유 · 소아에 흔히 발생하는 소양감과 전형적인 피부염증 반응을 주증상으로 하는 만성 재발성 피부 염증질환이다1). 아토피 피부염의 원인은 아직 정확하게 밝혀지지 않았으나2) IgE 합성과 호산구 증가와 함께 T세포 면역반응에서 Th1 반응에 비해 Th2 반응이 우세하여 발생된 불균형이 핵심적인 면역학적 원인으로 보고되고 있다3-4). Th2 세포가 분비하는 IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, TNF-α 등의 일련의 사이토카인 중 IL-4와 IL-13은 B세포의 IgE 생성을 유도하고, IL-5는 호산구의 분화와 성숙 및 분비에 관여 하여 즉시형 아토피 반응을 유발한다5-6).
	즉시형 아토피가 유발되는 Th1 반응 보다 Th2 반응이 우세하게 되는 과정은?	아토피 피부염의 원인은 아직 정확하게 밝혀지지 않았으나2) IgE 합성과 호산구 증가와 함께 T세포 면역반응에서 Th1 반응에 비해 Th2 반응이 우세하여 발생된 불균형이 핵심적인 면역학적 원인으로 보고되고 있다3-4). Th2 세포가 분비하는 IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, TNF-α 등의 일련의 사이토카인 중 IL-4와 IL-13은 B세포의 IgE 생성을 유도하고, IL-5는 호산구의 분화와 성숙 및 분비에 관여 하여 즉시형 아토피 반응을 유발한다5-6). 또한 IL-4, IL-5, IL-13 등의 사이토카인은 염증반응을 확장시켜 Th2 세포 반응을 우세하게 만드는 양성 되먹임 작용을 일으킨다6-7)

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연교(連翹) n-BuOH 분획물의 아토피 피부염 억제 효과
The Effects of Forsythiae Fructus n-BuOH Fraction on Atopic Dermatitis 원문보기

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연교(連翹) n-BuOH 분획물의 아토피 피부염 억제 효과 The Effects of Forsythiae Fructus n-BuOH Fraction on Atopic Dermatitis 원문보기

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