[논문]고삼 추출물의 피부장벽 강화와 염증완화 효과

노경백; 신승우; 윤소현; 원진배; 오세영; 김준오; 박덕훈; 정은선

doi:10.15230/scsk.2020.46.4.361

고삼 추출물의 피부장벽 강화와 염증완화 효과
Effect of Sophora flavescens Extract on Reinforcing Skin Barrier and Alleviating Inflammation 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.46 no.4, 2020년, pp.361 - 369

노경백 (바이오스펙트럼(주) 생명과학연구소) , 신승우 (바이오스펙트럼(주) 생명과학연구소) , 윤소현 (바이오스펙트럼(주) 생명과학연구소) , 원진배 (바이오스펙트럼(주) 생명과학연구소) , 오세영 (바이오스펙트럼(주) 생명과학연구소) , 김준오 ((주)신세계인터내셔날) , 박덕훈 (바이오스펙트럼(주) 생명과학연구소) , 정은선 (바이오스펙트럼(주) 생명과학연구소)

초록
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아토피성 피부염은 피부장벽 기능장애, 염증 및 만성 소양증을 특징으로 하는 다인성의 염증성 피부질환이다. 아토피성 피부염은 유전적, 면역학적, 환경적 요인 등의 복합적인 요인으로 피부장벽 기능과 면역기능의 장애를 유발한다고 알려져 있다. 고삼 추출물은 중국전통의학에서 사용되고 있으나, 이의 항아토피 효능에 대한 연구는 거의 진행되지 않았다. 본 연구에서는 아토피성 피부염의 주요 증상인 피부장벽 기능과 면역이상 개선에 대한 고삼추출물의 효과를 평가하였다. 고삼추출물은 피부장벽 기능에서 중요한 역할을 하는 각질세포막의 형성을 강화하는 결과를 나타내었다. 또한 피부의 보습작용에 있어서 중요한 히알루론산의 발현을 증가시키는 결과를 나타내었다. 아토피성 피부염 병변에서 특이적으로 증가하는 황색포도상구균에 대한 고삼추출물의 효능도 확인하였으며, 고삼추출물이 황색포도상구균으로부터 유도된 전염증성사이토카인의 생성을 억제함을 확인하였다. 또한 피부 스트레스 등으로 부터 생성되는 신경전달 물질인 substance P에 의해 유도된 전염증성사이토카인의 발현도 억제하는 것을 확인하였다. 이러한 결과들은 고삼추출물이 피부장벽기능과 면역반응 개선을 통해 아토피 피부염 치료에 사용될 수 있는 잠재적 후보물질임을 제시한다.

Abstract ▼ AI-Helper

Atopic dermatitis (AD) is a common and multifactorial inflammatory skin disease that is characterized by skin barrier dysfunction, inflammation, and chronic pruritus. AD has a complex etiology that includes genetic, immunological, and environmental factors that cause skin barrier abnormalities and immune dysfunctions. Sophora flavescens (SF) has been used in traditional Chinese medicine, but little research has been conducted on its anti-AD efficacy. In this study, we evaluated the effect of SF extract (SFE) on improving skin barrier function and immune abnormalities, which are the main symptoms of AD. SFE has the capacity to enhance the formation of cornified envelope (CE) that plays an important role in the skin barrier function. In addition, it was confirmed that SFE increased the expression of hyaluronic acid related to skin moisture. The effect of SFE against Staphylococcus aureus (S. aureus), which increases specifically in AD lesions, confirmed that SFE inhibited the production of pro-inflammatory cytokines induced by S. aureus. Furthermore, SFE was shown to inhibit the expression of pro-inflammatory cytokines induced by substance P (SP), the cause of skin neurogenic inflammation. These results demonstrate that SFE could be one of potential candidate agent for the treatment of AD by improving the skin barrier function and immune responses.

주제어

표/그림 (4)

그림 Figure 1. SFE increases CE formation in HaCaT. The relative CE content was expressed as the absorbance at 310 nm/mg protein (% of control). The results are expressed as mean ± SD (N = 3). *p < 0.001 versus the non-treated control.
그림 Figure 2. SFE increases hyaluronic acid expression in HaCaT. After 24 h treatment of (25, 50, and 100) μg/mL SFE, HaCaT cultured media was collected and the amount of hyaluronic acid was analyzed by ELISA. RA (10 μM) was used as a positive control. The results are expressed as mean ± SD (N = 3). *p < 0.01 versus the non-treated control; **p < 0.05 versus the non-treated control.
그림 Figure 3. SFE decreases the production of pro-inflammatory cytokines induced by S. aureus-cultured media. THP-1 was pretreated with (10, 50, 100, and 200) μg/mL SFE for 1 h, and then treated with S. aureus-cultured media for 24 h. THP-1 cultured media was collected, and the expression of IL-1β (A) and TNF-α (B) was analyzed by ELISA. The results are expressed as mean ± SD (N = 3). *p < 0.01 versus S. aureus CS untreated control; **p < 0.001 versus S. aureus CS untreated control; ***p < 0.05 versus S. aureus CS treated control; ****p < 0.01 versus S. aureus CS treated control.
그림 Figure 4. SFE decreases the production of pro-inflammatory cytokines induced by SP. THP-1 was pretreated with (10, 50, and 100) μg/mL SFE for 1 h, and then treated with SP for 24 h. THP-1 cultured media was collected, and the expression of IL-8 (A) and TNF-α (B) was analyzed by ELISA. The results are expressed as mean ± SD (N = 3). *p < 0.01 versus SP untreated control; **p < 0.05 versus SP treated control; ***p < 0.01 versus SP treated control.

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문제 정의

In this study, we demonstrated the effect of SFE on reinforcing skin barrier and alleviating inflammation that play an important role in the pathophysiology of AD. Therefore, SFE is expected to improve AD symptoms by modulating various underlying causes.

제안 방법

SFE increases hyaluronic acid expression in HaCaT. After 24 h treatment of (25, 50, and 100) μg/mL SFE, HaCaT cultured media was collected and the amount of hyaluronic acid was analyzed by ELISA. RA (10 μM) was used as a positive control.
Many of the unique proteins required for keratinocytes differentiation, including involucrin, loricrin, filaggrin, and keratins, are regulated by calcium[24]. To determine whether SFE improves skin barrier functions, we evaluated the effect of enhancing CE formation by SFE. Calcium was used as a positive control as a well-known modulator of keratinocyte differentiation[24].

데이터처리

Statistical significance of data was determined by a Studentʼ s t-test. All results were expressed as the means ± standard deviation (N = 3).

후속연구

Therefore, the anti-inflammatory effects observed in this study may be due to the presence of matrine or oxymatrine, quinolizidine alkaloids in SFE. The action of matrine or oxymatrine, the major components of SFE, on the anti-inflammatory and skin barrier-strengthening effects of this model should be confirmed through further studies.
Therefore, SFE is expected to improve AD symptoms by modulating various underlying causes. This study showed the potential of SFE as a therapeutic agent for AD, and further studies will be needed to clarify its clinical efficacy.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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