6종류의 배지를 이용한 135일 동안 8번의 박테리아상 분석을 통하여 새우젓갈 숙성 관련 우점종을 결정하였다. Marine agar를 배양에 사용하였을 경우, 가장 많은 수의 박테리아가 검출되었고, 숙성 65일차에 그 수가 2.51 × 107 CFU/g에 달하였다. 순수분 리된 총 467균주는 42속 87종으로 동정되었고, 16균주는 기존의 알려진 박테리아와 상동성을 나타내지 않았다. 1일차 분석에서 39종이 검출되었던 박테리아는 135일차에 13종으로 감소하여 우점화가 진행됨을 학인하였다. 속 수준에서의 우점은 Staphylococcus, Salimicrobium, Kocuria, Psychrobacter 순으로 나타났고, 1일차 분리균주의 2% 수준을 차지하던 Staphylococcus, Salimicrobium 속 균주는 135일차에 각각 39%와 36%에 달하였다. 우점종으로 확인된 Staphylococcus equorum, Salimicrobium salexigens, Kocuria palustris는 각각 전체 분리균주의 23.6%, 16.1%, 10.9%을 차지하였다. St. equorum와 Sm. salexigens는 21% (w/v) NaCl 농도에서의 생육이 확인되어 새우젓갈 숙성에 중요한 역할을 할 것으로 추정된다.
6종류의 배지를 이용한 135일 동안 8번의 박테리아상 분석을 통하여 새우젓갈 숙성 관련 우점종을 결정하였다. Marine agar를 배양에 사용하였을 경우, 가장 많은 수의 박테리아가 검출되었고, 숙성 65일차에 그 수가 2.51 × 107 CFU/g에 달하였다. 순수분 리된 총 467균주는 42속 87종으로 동정되었고, 16균주는 기존의 알려진 박테리아와 상동성을 나타내지 않았다. 1일차 분석에서 39종이 검출되었던 박테리아는 135일차에 13종으로 감소하여 우점화가 진행됨을 학인하였다. 속 수준에서의 우점은 Staphylococcus, Salimicrobium, Kocuria, Psychrobacter 순으로 나타났고, 1일차 분리균주의 2% 수준을 차지하던 Staphylococcus, Salimicrobium 속 균주는 135일차에 각각 39%와 36%에 달하였다. 우점종으로 확인된 Staphylococcus equorum, Salimicrobium salexigens, Kocuria palustris는 각각 전체 분리균주의 23.6%, 16.1%, 10.9%을 차지하였다. St. equorum와 Sm. salexigens는 21% (w/v) NaCl 농도에서의 생육이 확인되어 새우젓갈 숙성에 중요한 역할을 할 것으로 추정된다.
To determine the dominant bacterial species during the Saeu-jeotgal ripening process, the cultivable bacterial population was examined over a 135-day period using six different growth media. The greatest numbers of bacteria were identified when marine agar was used for culture, with maximum cell den...
To determine the dominant bacterial species during the Saeu-jeotgal ripening process, the cultivable bacterial population was examined over a 135-day period using six different growth media. The greatest numbers of bacteria were identified when marine agar was used for culture, with maximum cell density identified at day 65 (2.51 × 107 colony forming units/g). Over the course of 135 days, the bacterial diversity was analyzed eight times. A total of 467 isolates, comprising 87 species from 42 genera, as well as 16 isolates belonging to previously unknown species, were identified. The number of species detected decreased from 39 at day 1 to 13 at day 135. The order of dominance at the genus level was as follows: Staphylococcus, Salimicrobium, Kocuria, and Psychrobacter. Staphylococcus and Salimicrobium accounted for 2% of the diversity at day 1, and then increased to 39% and 36%, respectively, at day 135. The dominant species Staphylococcus equorum, Salimicrobium salexigens, and Kocuria palustris accounted for 23.6%, 16.1%, and 10.9% of all isolates, respectively. Importantly, both St. equorum and Sm. salexigens remained viable at a NaCl concentration of 21% (w/v), which indicates their strong involvement in the ripening of Saeu-jeotgal.
To determine the dominant bacterial species during the Saeu-jeotgal ripening process, the cultivable bacterial population was examined over a 135-day period using six different growth media. The greatest numbers of bacteria were identified when marine agar was used for culture, with maximum cell density identified at day 65 (2.51 × 107 colony forming units/g). Over the course of 135 days, the bacterial diversity was analyzed eight times. A total of 467 isolates, comprising 87 species from 42 genera, as well as 16 isolates belonging to previously unknown species, were identified. The number of species detected decreased from 39 at day 1 to 13 at day 135. The order of dominance at the genus level was as follows: Staphylococcus, Salimicrobium, Kocuria, and Psychrobacter. Staphylococcus and Salimicrobium accounted for 2% of the diversity at day 1, and then increased to 39% and 36%, respectively, at day 135. The dominant species Staphylococcus equorum, Salimicrobium salexigens, and Kocuria palustris accounted for 23.6%, 16.1%, and 10.9% of all isolates, respectively. Importantly, both St. equorum and Sm. salexigens remained viable at a NaCl concentration of 21% (w/v), which indicates their strong involvement in the ripening of Saeu-jeotgal.
At each time point, 150 g of sample were ground using an IKA Ultra-Turrax homogenizer T-18 (IKA, Germany) and then filtered through sterilized gauze. The NaCl content was measured according to the Mohr method [4], and the pH was measured using a pH meter. The filtrates were then spread on agar plates after appropriate dilutions in saline.
kr/~jchun/phydit/). Evolutionary distances were calculated according to the Kimura two-parameter model [22], and clustering was performed using the neighbor-joining method [32]. A phylogenetic tree was generated using a treeing algorithm contained within the PHYDIT program.
성능/효과
The results of the current study were compared with both previous culture-independent studies of Saeu-jeotgal fermentation to identify the dominant species and bacterial succession during fermentation. Fortunately, all three studies used samples ripened at approximately 15℃, which is the average storage temperature of the high quality Saeu-jeotgal produced in the Kwangchun area [21].
후속연구
A history of use in food fermentation will help to implement St. equorum strains in the jeotgal industry, but further proof of their safety is required.
참고문헌 (41)
Abdeljabbar H, Cayol JL, Ben Hania W, Boudabous A, Sadfi N, Fardeau ML. 2013. Halanaerobium sehlinense sp. nov., an extremely halophilic, fermentative, strictly anaerobic bacterium from sediments of the hypersaline lake Sehline Sebkha. Int. J. Syst. Evol. Microbiol. 63: 2069-2074.
Akolkar AV, Durai D, Desai AJ. 2010. Halobacterium sp. SP1(1) as a starter culture for accelerating fish sauce fermentation. J. Appl. Microbiol. 109: 44-53.
Ammor MS, Mayo B. 2007. Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production: An update. Meat Sci. 76: 138-146.
AOAC. 2000. Official methods of analysis. 17th ed. Association of Official Analytical Chemist. Washington, D.C., USA.
Beddows CG, Ardeshir AG. 1979. The production of soluble fish protein solution for use in fish sauce manufacture I. The use of added enzymes. Int. J. Food Sci. Technol. 14: 603-612.
Bhupathiraju VK, McInerney MJ, Woese CR, Tanner RS. 1999. Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine. Int. J. Syst. Bacteriol. 49: 953-960.
Blaiotta G, Pennacchia C, Villani F, Ricciardi A, Tofalo R, Parente E. 2004. Diversity and dynamics of communities of coagulase-negative staphylococci in traditional fermented sausages. J. Appl. Microbiol. 97: 271-284.
Corbiere Morot-Bizot S, Leroy S, Talon R. 2006. Staphylococcal community of a small unit manufacturing traditional dry fermented sausages. Int. J. Food Microbiol. 108: 210-217.
Gildberg A. 2001. Utilisation of male Arctic capelin and Atlantic cod intestines for fish sauce production - evaluation of fermentation conditions. Bioresour. Technol. 76: 119-123.
Gildberg A, Espejo-Hermes J, Magno-Orejana F. 1984. Acceleration of autolysis during fish sauce fermentation by adding acid and reducing the salt content. J. Sci. Food Agri. 35: 1363-1369.
Guan L, Cho KH, Lee JH. 2011. Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria. Food Microbiol. 28: 101-113.
Han KI, Kim YH, Hwang SG, Jung EG, Patnaik BB, Han YS, et al. 2014. Bacterial community dynamics of salted and fermented shrimp based on denaturing gradient gel electrophoresis. J. Food Sci. 79: M2516-M2522.
Hiraga K, Nishikata Y, Namwong S, Tanasupawat S, Takada K, Oda K. 2005. Purification and characterization of serine proteinase from a halophilic bacterium, Filobacillus sp. RF2-5. Biosci. Biotechnol. Biochem. 69: 38-44.
Jeon CO, Lim JM, Lee JM, Xu LH, Jiang CL, Kim CJ. 2005. Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int. J. Syst. Evol. Microbiol. 55: 1891-1896.
Jeong DW, Kim HR, Han S, Jeon CO, Lee JH. 2013. A proposal to unify two subspecies of Staphylococcus equorum: Staphylococcus equorum subsp. equorum and Staphylococcus equorum subsp. linens. Anton. Van. Leeuw. 104: 1049-1062.
Jung JY, Lee SH, Lee HJ, Jeon CO. 2013. Microbial succession and metabolite changes during fermentation of saeu-jeot: traditional Korean salted seafood. Food Microbiol. 34: 360-368.
Kim AJ, Park SY, Choi JW, Park SH, Ha SD. 2006. Assessment of microbial contamination and nutrition of Kwangchun Shrimp Jeotgal (Salt Fermented Shrimp). Korean J. Food Sci. Technol. 38: 121-127.
Kimura M. A 1980. Simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120.
Klomklao S, Benjakul S, Visessanguan W, Kishimura H, Simpson BK. 2006. Effects of the addition of spleen of skipjack tuna (Katsuwonus pelamis) on the liquefaction and characteristics of fish sauce made from sardine (Sardinella gibbosa). Food Chem. 98: 440-452.
Lee KH, Kim JH, Cha BS, Kim JO, Byun MW. 1999. Quality evaluationof commercial salted and fermented seafoods. Korean J. Food Sci. Technol. 31: 1427-1433.
Lopetcharat K, Park JW. 2002. Characteristics of fish sauce madefrom pacific whiting and surimi by-products during fermentationstage. J. Food Sci. 67: 511-516.
Mauriello G, Casaburi A, Blaiotta G, Villani F. 2004. Isolation and technological properties of coagulase negative staphylococci from fermented sausages of Southern Italy. Meat Sci. 67: 149-158.
Meugnier H, Bes M, Vernozy-Rozand C, Mazuy C, Brun Y, Freney J, et al. 1996. Identification and ribotyping of Staphylococcus xylosus and Staphylococcus equorum strains isolated from goat milk and cheese. Int. J. Food Microbiol. 31: 325-331.
Montel MC, Masson F, Talon R. 1998. Bacterial role in flavour development. Meat Sci. 49S1: S111-S123.
Oh SH, Heo OS, Bang OK, Chang HC, Shin H-S, Kim MR. 2004. Microbiological safety of commercial salt-fermented shrimp during storage. Korean J. Food Sci. Technol. 36: 507-513.
Roh SW, Kim KH, Nam YD, Chang HW, Park EJ, Bae JW. 2010. Investigation of archaeal and bacterial diversity in fermented seafood using barcoded pyrosequencing. ISME J. 4: 1-16.
Sinsuwan S, Rodtong S, Yongsawatdigul J. 2007. NaCl-activated extracellular proteinase from Virgibacillus sp. SK37 isolated from fish sauce fermentation. J. Food Sci. 72: C264-C269.
Sinsuwan S, Rodtong S, Yongsawatdigul J. 2008. Characterization of Ca 2+ -activated cell-bound proteinase from Virgibacillus sp. SK37 isolated from fish sauce fermentation. LWT - Food Sci. Technol. 41: 2166-2174.
Sinsuwan S, Rodtong S, Yongsawatdigul J. 2008. Production and characterization of NaCl-activated proteinases from Virgibacillus sp. SK33 isolated from fish sauce fermentation. Process Biochem. 43: 185-192.
Siringan P, Raksakulthai N, Yongsawatdigul J. 2006. Autolytic activity and biochemical characteristics of endogenous proteinasesin Indian anchovy (Stolephorus indicus). Food Chem. 98: 678-684.
Tian X-P, Dastager SG, Lee JC, Tang SK, Zhang YQ, Park DJ, et al. 2007. Alkalibacillus halophilus sp. nov., a new halophilic species isolated from hypersaline soil in Xin-Jiang province, China. Syst. Appl. Microbiol. 30: 268-272.
Udomsil N, Rodtong S, Choi YJ, Hua Y, Yongsawatdigul J. 2011. Use of Tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation. J. Agri. Food Chem. 59: 8401-8408.
Yongsawatdigul J, Rodtong S, Raksakulthai N. 2007. Acceleration of Thai fish sauce fermentation using proteinases and bacterial starter cultures. J. Food Sci. 72: M382-M390.
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