탈모마우스모델에서의 송지추출물 및 그 성분인 아비에트산의 모발성장효과 Hair Growth-promoting Effect of Resina Pini and Its Main Constituent, Abietic Acid, in Mouse Model of Alopecia원문보기
최근 남성성탈모증에 대한 관심이 증대되고 있으며, 이에 천연물 및 그의 활성성분을 활용한 새로운 약물 개발에 대한 연구가 증가하고 있다. 송지(Resina Pini, RP)는 Pinus sp. (Pinaceae)의 수지질로 전통의학적으로 감염, 우식증, 치주질환에 사용되어왔다. 본 연구진은 RP의 성분인 아비에트산(abietic acid, AA)이 남성성탈모기전에 중요한 효소인 $5{\alpha}$-reductase를 억제하는 효과를 세포 수준에서 입증한바 있으며, 이번 연구에서는 실제로 탈모억제 및 모발 성장에 대하여 실험동물 수준에서 입증하고자 한다. C3H/HeN 탈모마우스 모델에서 RP는 300 mg/kg에서 유의하게 탈모억제를 확인하였으며, 뿐만 아니라 AA는 30 mg/kg에서도 유의하게 탈모억제효과를 보였다. 이상의 결과로부터 RP는 그 활성성분인 AA가 $5{\alpha}$-reductase 억제하는 기전을 통해 남성성탈모억제효과를 보였다고 사료되며, 향후 탈모억제 보완치료법으로의 이용 가능성을 보였다.
최근 남성성탈모증에 대한 관심이 증대되고 있으며, 이에 천연물 및 그의 활성성분을 활용한 새로운 약물 개발에 대한 연구가 증가하고 있다. 송지(Resina Pini, RP)는 Pinus sp. (Pinaceae)의 수지질로 전통의학적으로 감염, 우식증, 치주질환에 사용되어왔다. 본 연구진은 RP의 성분인 아비에트산(abietic acid, AA)이 남성성탈모기전에 중요한 효소인 $5{\alpha}$-reductase를 억제하는 효과를 세포 수준에서 입증한바 있으며, 이번 연구에서는 실제로 탈모억제 및 모발 성장에 대하여 실험동물 수준에서 입증하고자 한다. C3H/HeN 탈모마우스 모델에서 RP는 300 mg/kg에서 유의하게 탈모억제를 확인하였으며, 뿐만 아니라 AA는 30 mg/kg에서도 유의하게 탈모억제효과를 보였다. 이상의 결과로부터 RP는 그 활성성분인 AA가 $5{\alpha}$-reductase 억제하는 기전을 통해 남성성탈모억제효과를 보였다고 사료되며, 향후 탈모억제 보완치료법으로의 이용 가능성을 보였다.
Recently, increased attention has been directed toward medicinal extracts and their active ingredients as potential new drug candidates for androgenic alopecia. Resina Pini (RP), a resinous exudation obtained from Pinus sp. (Pinaceae), has been used as a traditional medicine for the treatment of inf...
Recently, increased attention has been directed toward medicinal extracts and their active ingredients as potential new drug candidates for androgenic alopecia. Resina Pini (RP), a resinous exudation obtained from Pinus sp. (Pinaceae), has been used as a traditional medicine for the treatment of infection, pain related to dental caries, and periodontal disease. Previously, we suggested that RP and its main constituent, abietic acid (abieta-7,13-dien-18-oic acid; AA), may play important roles against androgenic alopecia as $5{\alpha}$-reductase inhibitors. However, to date, there is no evidence that AA has hair growth-promoting effects in vivo. In this study, we found that 10 ~ 300 mg/kg RP and 3 ~ 30 mg/kg AA significantly promoted hair growth in a C3H/HeN mouse model of alopecia. To our knowledge, this is the first report of the hair growth-promoting effects of RP and AA in vivo. From these results, RP and its main constituent AA can promote hair growth in mouse by inhibiting $5{\alpha}$-reductase activity and may be effective alternative therapies for androgenic alopecia.
Recently, increased attention has been directed toward medicinal extracts and their active ingredients as potential new drug candidates for androgenic alopecia. Resina Pini (RP), a resinous exudation obtained from Pinus sp. (Pinaceae), has been used as a traditional medicine for the treatment of infection, pain related to dental caries, and periodontal disease. Previously, we suggested that RP and its main constituent, abietic acid (abieta-7,13-dien-18-oic acid; AA), may play important roles against androgenic alopecia as $5{\alpha}$-reductase inhibitors. However, to date, there is no evidence that AA has hair growth-promoting effects in vivo. In this study, we found that 10 ~ 300 mg/kg RP and 3 ~ 30 mg/kg AA significantly promoted hair growth in a C3H/HeN mouse model of alopecia. To our knowledge, this is the first report of the hair growth-promoting effects of RP and AA in vivo. From these results, RP and its main constituent AA can promote hair growth in mouse by inhibiting $5{\alpha}$-reductase activity and may be effective alternative therapies for androgenic alopecia.
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제안 방법
All experiments were conducted 12 h after the cells had been seeded on the 96- and 24-well plates at densities of 1 ×104 and 2 × 104 cells/well respectively.
Although RP have inhibitory effects in vitro 5α-reductase assay, its potential hair growthpromoting effect has not been tested in vivo animal model. Therefore, in this study, we applied the hair growthpromoting activity of RP and AA in alopecia models of C3H/HeN mice. The hair growth indexes of 10 ∼ 300 mg/kg RP and 3 ∼ 30 mg/kg AA groups were slight than that of the vehicle group after treatment for 3 weeks, indicating the hair growth-promoting activity of RP extract and AA (Figure 1, 2 and Table 1).
대상 데이터
Dulbecco’s modified Eagle’s medium (DMEM), penicillin / streptomycin (P/S) and fetal bovine serum (FBS), were purchased from Gibco (MD, USA).
Dulbecco’s modified Eagle’s medium (DMEM), penicillin / streptomycin (P/S) and fetal bovine serum (FBS), were purchased from Gibco (MD, USA). Minoxidil, ethanol, MTT, and dimethylsulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA). Also, PEG2 was purchased from Abcam (Cambridge, MA).
데이터처리
The statistical variables were analyzed using a one-way analysis of variance (ANOVA) and post hoc multiple mean comparisons (Tukey’s HSD test).
성능/효과
Treatment with LPS significantly increased PGE2 (by 19.55 ± 0.54%) and nitric oxide (by 11.97 ± 0.28%) compared with the control, while treatment with RP and AA reduced LPS-induced PGE2 (by 13.50 ± 0.59% to 9.34 ± 0.23% and 14.56 ±0.47% to 9.96 ± 0.27%) and nitric oxide (by 11.06 ±0.16% to 5.60 ± 0.12% and 9.73 ± 0.27% to 4.79 ±0.29%) (Figure 3A, B, C).
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