A total of 121 species of lichens belonging to the genus Arthothelium have been described to date, most of which have been found in tropical regions. Here, we describe the discovery of a novel Arthothelium species for the first time in South Korea. Until now, Arthothelium ruanum was the only Arthoth...
A total of 121 species of lichens belonging to the genus Arthothelium have been described to date, most of which have been found in tropical regions. Here, we describe the discovery of a novel Arthothelium species for the first time in South Korea. Until now, Arthothelium ruanum was the only Arthothelium species reported in South Korea. Among the 113 specimens collected in this study, we identified A. ruanum and a putative new species, Arthothelium punctatum (J. S. Park & J.-S. Hur, sp. nov.). The diagnostic characters of A. punctatum are as follows: apothecia punctate, shortly elongate to branched, small, 0.1-0.2 mm wide, hypothecium hyaline to pale brown and obovate to broadly ellipsoid, muriform ascospores, $29.5-44.6{\times}12.2-18.2{\mu}m$. The new species was found in Mt. Seokbyeong at an altitude of 790 m on smooth bark. Upon phylogenic analysis, the putative new species, A. punctatum, was separated from other Arthothelium species although the specimens analyzed were clustered with Arthoniaceae in phylogenetic trees based on both the mitochondrial small subunit (mtSSU) sequence and combined mtSSU and nuclear ribosomal large subunit sequences. Our data clearly indicate that this species is a new species belonging to the family Arthoniaceae. To elucidate the taxonomic characteristics of the new species, we provide morphological descriptions and a distribution map.
A total of 121 species of lichens belonging to the genus Arthothelium have been described to date, most of which have been found in tropical regions. Here, we describe the discovery of a novel Arthothelium species for the first time in South Korea. Until now, Arthothelium ruanum was the only Arthothelium species reported in South Korea. Among the 113 specimens collected in this study, we identified A. ruanum and a putative new species, Arthothelium punctatum (J. S. Park & J.-S. Hur, sp. nov.). The diagnostic characters of A. punctatum are as follows: apothecia punctate, shortly elongate to branched, small, 0.1-0.2 mm wide, hypothecium hyaline to pale brown and obovate to broadly ellipsoid, muriform ascospores, $29.5-44.6{\times}12.2-18.2{\mu}m$. The new species was found in Mt. Seokbyeong at an altitude of 790 m on smooth bark. Upon phylogenic analysis, the putative new species, A. punctatum, was separated from other Arthothelium species although the specimens analyzed were clustered with Arthoniaceae in phylogenetic trees based on both the mitochondrial small subunit (mtSSU) sequence and combined mtSSU and nuclear ribosomal large subunit sequences. Our data clearly indicate that this species is a new species belonging to the family Arthoniaceae. To elucidate the taxonomic characteristics of the new species, we provide morphological descriptions and a distribution map.
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제안 방법
, Seongnam, Korea). Sequencing analysis was performed.
대상 데이터
1). Samples were collected from the southwestern portion of the Korean peninsula (41 specimens) and a northeastern mountainous area (25 specimens), Jeju province (41 specimens) and Ulleung Island (6 specimens).
The maximum likelihood (ML) method was selected to construct a phylogenetic tree. The Kimura 2-parameter model was selected and the reliability of the inferred tree was tested by 1,000 bootstrap replications. Dothidea sambuci (mtSSU, AY544739; nuLSU, AY544681) and Pleospora herbarum (mtSSU, FJ190610; nuLSU, DQ247804) were selected as outgroups based on BLAST comparisons of the sequences available in the GenBank database.
성능/효과
The combined data matrix was composed of 3,727 characters, 2,201 of which were variable and 823 characters that were conserved. As with the ML tree based on the mtSSU sequences (Fig. 2), two specimens from a putative new species, A. punctatum, were separated from the other Arthothelium species (Fig. 3), indicating that A. punctatum would be a new species. In summary, we identified two different Arthothelium species, A.
punctatum were included in Arthoniaceae, which was supported by highconfidence bootstrap values. The three A. ruanum specimens were clustered with A. ruanum (previously referred to as Arthonia ruana) (Fig. 2). Interestingly, the specimens of the putative new species, A.
For phylogenetic analysis, we included sequences of 40 additional specimens from GenBank representing 35 taxa. Therefore, a total of 37 taxa and 45 specimens of Arthoniaceae, Opegraphaceae, and R℃ellaceae were used. The mtSSU matrix included 1,140 characters, 545 of which were variable and 389 that were conserved.
참고문헌 (20)
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