[논문]Candelaria asiatica, an Ignored New Species from South Korea

Liu, Dong; Hur, Jae-Seoun

doi:10.1080/12298093.2018.1538070

Candelaria asiatica, an Ignored New Species from South Korea 원문보기

Mycobiology, v.46 no.4, 2018년, pp.305 - 310

Liu, Dong (Korean Lichen Research Institute (KoLRI), Sunchon National University) , Hur, Jae-Seoun (Korean Lichen Research Institute (KoLRI), Sunchon National University)

Abstract ▼ AI-Helper

The genus Candelaria is characterized by a micro-foliose to micro-fruticose thallus and contains eight species, two of which were reported in South Korea. During the excursion of a Korean lichen flora investigation, some suspected Candelaria concolor specimens were collected, and their morphological, chemical, molecular phylogenetic, and geographic analyses were conducted. The samples eventually proved to be a new species, Candelaria asiatica, which can be recognized by a small, yellow lobate thallus with a pulverulent surface, and a fragile lobe margin with blastidia or phyllidia-like lobules.

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제안 방법

Three specimens in this study were collected from South Korea and deposited at Sunchon National University, Korean Lichen Research Institute(KoLRI). Morphological and anatomical characters were recorded under a dissecting microscope(Nikon SMZ 745 T, Tokyo, Japan) and an Olympus BX 50 microscope (Olympus, Tokyo, Japan), and images were captured using an HD-Measure LTHS-300 (Leetech Co., Seoul, South Korea) microscope and a Carl Zeiss MicroImaging with Axio Cam ERc5 s imaging system (Carl Zeiss MicroImaging, GmbH 37081, Gottingen, Germany). The secondary metabolites were studied using the spot test (K = 10% aqueous KOH solution; C = saturated aqueous Ca(ClO)₂; KC = 10% aqueous KOH solution followed by saturated aqueous Ca(ClO)₂; PD = 5% alcoholic p-phenylenediamine solution)and thin-layer chromatography (TLC) in solvent C [15,16].

대상 데이터

Three specimens in this study were collected from South Korea and deposited at Sunchon National University, Korean Lichen Research Institute(KoLRI). Morphological and anatomical characters were recorded under a dissecting microscope(Nikon SMZ 745 T, Tokyo, Japan) and an Olympus BX 50 microscope (Olympus, Tokyo, Japan), and images were captured using an HD-Measure LTHS-300 (Leetech Co.
The secondary metabolites were studied using the spot test (K = 10% aqueous KOH solution; C = saturated aqueous Ca(ClO)₂; KC = 10% aqueous KOH solution followed by saturated aqueous Ca(ClO)₂; PD = 5% alcoholic p-phenylenediamine solution)and thin-layer chromatography (TLC) in solvent C [15,16]. UV tests were performed using a UV Chamber (CE07 21470) under long(366 nm) wavelength.

이론/모형

ML bootstrap values were estimated from consensus trees obtained from 1000 nonparametric bootstrapping pseudoreplicates; a clade of bootstrap value >70% indicates crudely supported. Bayesian inference (BI) was performed with MrBayes v3.1.2 [25], applying the best-fitted substitution models(GTR+I+G) based on the Akaike information criterion using jModelTest 3.7 [26]. Bayesian inferences were conducted using four chains and run for 2 million generations.

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