된장은 콩 발효식품으로 주원료인 콩류에 Bacillus subtilis, Rizopus, Mucor와 Aspergillus species를 접종하여 발효시킨 메주를 소금물과 혼합하여 숙성 시킨 한국의 전통적인 발효식품이다. 본 연구에서는 동물실험을 통하여 검정콩 된장의 항비만 효과를 확인하였다. 항비만 효과의 확인은 혈중 TG, TC, 아디포넥틴과 렙틴의 레벨을 측정함과 동시에 지방합성에 관여하는 전사인자인 SREBP-1c과 PPAR-g의 mRNA와 단백질 발현 정도를 측정하였다. 고지방 식이에 검정콩 된장을 첨가한 그룹에서는 고지방식이로 인해 증가된 체중을 유의적으로 감소시킴을 확인하였다. 혈중 중성지방, 콜레스테롤과 렙틴의 레벨은 고지방식이를 섭취한 마우스에 비하여 검정콩 된장을 섭취한 마우스에서 감소하였으며 아디포넥틴의 분비량은 유의적으로 증가하였다. 이러한 결과가 지방 생성의 억제로부터 유도되는지를 조사하기 위하여 지방 합성에 관여하는 전사인자인 SREBP-1c과PPAR-g의 mRNA양과 단백질 발현을 측정한 결과 검정콩 된장을 섭취한 마우스에서 현저하게 감소하는 것을 확인하였다. 이러한 결과는 검정 콩 된장의 섭취가 지방대사와 지방 전사 인자의 활성을 감소시킨다는 것을 확인함으로써 검정콩 된장이 비만의 예방과 진행을 개선시킬 수 있음을 증명하였다.
된장은 콩 발효식품으로 주원료인 콩류에 Bacillus subtilis, Rizopus, Mucor와 Aspergillus species를 접종하여 발효시킨 메주를 소금물과 혼합하여 숙성 시킨 한국의 전통적인 발효식품이다. 본 연구에서는 동물실험을 통하여 검정콩 된장의 항비만 효과를 확인하였다. 항비만 효과의 확인은 혈중 TG, TC, 아디포넥틴과 렙틴의 레벨을 측정함과 동시에 지방합성에 관여하는 전사인자인 SREBP-1c과 PPAR-g의 mRNA와 단백질 발현 정도를 측정하였다. 고지방 식이에 검정콩 된장을 첨가한 그룹에서는 고지방식이로 인해 증가된 체중을 유의적으로 감소시킴을 확인하였다. 혈중 중성지방, 콜레스테롤과 렙틴의 레벨은 고지방식이를 섭취한 마우스에 비하여 검정콩 된장을 섭취한 마우스에서 감소하였으며 아디포넥틴의 분비량은 유의적으로 증가하였다. 이러한 결과가 지방 생성의 억제로부터 유도되는지를 조사하기 위하여 지방 합성에 관여하는 전사인자인 SREBP-1c과PPAR-g의 mRNA양과 단백질 발현을 측정한 결과 검정콩 된장을 섭취한 마우스에서 현저하게 감소하는 것을 확인하였다. 이러한 결과는 검정 콩 된장의 섭취가 지방대사와 지방 전사 인자의 활성을 감소시킨다는 것을 확인함으로써 검정콩 된장이 비만의 예방과 진행을 개선시킬 수 있음을 증명하였다.
Doenjang is a traditional korean fermented soybean paste made from meju (fermented soybean), which are fermented by diverse microorganisms including Bacillus subtilis and molds such as Rizopus, Mucor, and Aspergillus species The purpose of this study was to investigate the antiobesity effect of the ...
Doenjang is a traditional korean fermented soybean paste made from meju (fermented soybean), which are fermented by diverse microorganisms including Bacillus subtilis and molds such as Rizopus, Mucor, and Aspergillus species The purpose of this study was to investigate the antiobesity effect of the black soybean doenjang (Korean fermented soybean pastes) in C57BL/6 mice. The anti-obesity effect was determined by measuring the release of adiponectin, leptin and adipogenic transcription factors by using reverse transcription-polymerase chain reaction and western blot. Weight gain was significantly reduced in the mice fed high fat diets (HFD) plus black soybean doenjang (HBD) compared to HFD mice. The HBD were also effective in improving the lipid profile. They significantly decreased the levels of serum triglyceride and cholesterol. In addition, they had a significantly down regulated impact on antiobesity factors; leptin level and increased adiponectin level. Also, mRNA and protein expression of two adipogenic transcription factors, SREBP-1c and $PPAR-{\gamma}$, in high fat with black soybean fed mice were markedly down regulated. These results indicate that the black soybean doenjang potentiates an anti-obesity effect by modulating lipid metabolism, thereby inhibiting adipogenic transcriptional activation.
Doenjang is a traditional korean fermented soybean paste made from meju (fermented soybean), which are fermented by diverse microorganisms including Bacillus subtilis and molds such as Rizopus, Mucor, and Aspergillus species The purpose of this study was to investigate the antiobesity effect of the black soybean doenjang (Korean fermented soybean pastes) in C57BL/6 mice. The anti-obesity effect was determined by measuring the release of adiponectin, leptin and adipogenic transcription factors by using reverse transcription-polymerase chain reaction and western blot. Weight gain was significantly reduced in the mice fed high fat diets (HFD) plus black soybean doenjang (HBD) compared to HFD mice. The HBD were also effective in improving the lipid profile. They significantly decreased the levels of serum triglyceride and cholesterol. In addition, they had a significantly down regulated impact on antiobesity factors; leptin level and increased adiponectin level. Also, mRNA and protein expression of two adipogenic transcription factors, SREBP-1c and $PPAR-{\gamma}$, in high fat with black soybean fed mice were markedly down regulated. These results indicate that the black soybean doenjang potentiates an anti-obesity effect by modulating lipid metabolism, thereby inhibiting adipogenic transcriptional activation.
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문제 정의
and SREBP-1c in the liver of the mice. Given this clinical relevance of black soybean doenjang to obesity, this study will provide evidence for lipid-lowering effects of dietary supplementation of black soybean doenjang for inducing weight loss in diet-induced obese mice.
To evaluate the beneficial effect of black soybean doenjang against obesity in male C57BL/6 mice, subjects were divided into four groups and maintained on a normal diet (ND), high fat diet (HFD), high fat diet with black soybean doengjang (HBD), high fat diet with yellow soybean doenjang (HYD) for 8 weeks. Thus, the objective of this study was to investigate the anti-obesity effect and lipid-regulating effects of black soybean doenjang in C57BL/6J mice.
가설 설정
In this study, we determined whether the black soybean doenjang improves the lipid profiles, gene and protein expression levels of obesity-related genes of PPAR-# and SREBP-1c in the liver of the mice.
제안 방법
The primer sequences specific for the genes examined were: GAPDH, forward primer, 5'-TGTGTCCGTCGTGGATCTGA-3, reverse primer, 5-CCTGCTTCACCACCTTCTTGAT-3'; SREBP-1c, forward primer, 5‘-AGCAGCCCCTAGAACAAACAC-3’, reverse primer, 5‘-CAGCAGTGAGTCTGCCTTGAT-3'; PPARγ, forward primer, 5’-TCGCTGATGCACTGCCTATG-3‘, reverse primer, 5’-GAGAGGTCCACAGAGCTGATT-3‘.
Fermented black soybean paste is a viable candidate to improve physiological function due to its high anthocyanin content compared to yellow soybean paste. The purpose of the present study was to verify the anti-obesity effects of dietary black soybean doenjang with in a population of high fat diet fed C57BL/6 mice. In this study, a significant reduction in body weight gain with black soybean doenjang supplementation indicates that the black soybean doenjang suppresses the HFD-induced increase in body weight gain and fat weight (Table 2, Table 3).
As already indicated, anthocyanins showed antiobestic effects [9, 26, 33, 35]. To evaluate the beneficial effect of black soybean doenjang against obesity in male C57BL/6 mice, subjects were divided into four groups and maintained on a normal diet (ND), high fat diet (HFD), high fat diet with black soybean doengjang (HBD), high fat diet with yellow soybean doenjang (HYD) for 8 weeks. Thus, the objective of this study was to investigate the anti-obesity effect and lipid-regulating effects of black soybean doenjang in C57BL/6J mice.
To investigate how lipogenesis are regulated in black soybean doenjang, we examined expression of PPAR-γ and SREBP-1 in the liver (Fig. 1, Fig. 2).
대상 데이터
Fifty 5-week-old male C57BL/6 mice, weighing approximately 14-16 g each, were purchased from the Korean Experimental Animal Center (Orient Co., Seoul, Korea). Mice were acclimated to the experimental facility for 1 week before they were divided into four groups of eight and placed in polycarbonate cages in a room maintained at 22±1℃ with 55±5% relative humidity.
데이터처리
Significant differences among the treatment means were determined using analysis of variance (ANOVA) and Duncan’s multiple range test at p<0.05.
이론/모형
Determination of leptin and adiponectin levels in the plasma was performed with sandwich enzyme-linked immunosorbent assay (ELISA). For the leptin analysis, anti-mouse leptin, recombinant mouse leptin, biotinylated anti-mouse leptin antibodies (MOB00), and adiponectin analysis, a kit (MRP300) was purchased from R&D Systems (R&D Systems, Minneapolis, USA).
성능/효과
After 8 weeks feeding, the leptin levels of HBD group was significantly lower than the HFD group by 31%(p<0.05).
The manifestation level of lipogenesis-related transcription factor such as mRNA in PPAR-γ and SREBP-1 along with protein was the lowest in the HBD group. From these results, it can be concluded that black soybean doenjang can contribute to an effective diet, by regulating the lipid metabolism and to bringing down the level of transcription factors related to fatty acid metabolism to normal levels. Therefore, black soybean doenjang can regulate the various complications caused by a high fat diet including weight increase, obesity of organ and fat tissue, lipid dysfunction, and lastly the manifestation level of transcription factors related to fatty acid metabolism.
Protein level in SREBP-1 increased the most in the HFD group, whereas it decreased in the HBD group. It was possible to confirm that the high fat diet of black soybean doenjang led to the decreased manifestation of transcription factors which take part in fat accumulation and lipid metabolism. In the initial measurement of weight change, there was similar level of decrease in the HBD group, whereas the level of TG, TC, leptin, and adiponectin in plasma measured lower in the HBD group than in the HFD group.
The TC level of ND, HBD and HYD group were significantly lower than the HFD group by approximately 31, 40 and 24% respectively (p<0.05).
The TG level in the ND, HBD and HYD group were significantly lower than the HFD group by 30, 40 and 24% respectively (p<0.05).
The epididymal fat pad weight of ND, HBD and HYD groups were significantly decreased by 45, 43 and 38%, respectively, compared to that of the HFD group (p<0.05).
The perirenal fat pad weight of ND, HBD, and HYD groups were significantly reduced by approximately 42, 42, and 38% respectively, compared to that of the HFD group (p<0.05).
후속연구
There has been reported that adiponectin has certain effect on insulin sensitivity the regulation of glucose [27]. Even though we did not measure glucose and insulin levels in this study which might be a limitation of this study, further studies are warranted to confirm the possible association between adiponectin and glucose homeostasis levels.
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