Atopic dermatitis (AD) is an inflammatory skin condition accompanied by symptoms such as edema and hemorrhage. Kimchi is a traditional fermented Korean dish consisting of various probiotics. In this study, the therapeutic effect of Lactobacillus plantarum CJLP55 isolated from Kimchi was studied in A...
Atopic dermatitis (AD) is an inflammatory skin condition accompanied by symptoms such as edema and hemorrhage. Kimchi is a traditional fermented Korean dish consisting of various probiotics. In this study, the therapeutic effect of Lactobacillus plantarum CJLP55 isolated from Kimchi was studied in AD-induced mice. Orally administered Lactobacillus strain, CJLP55, suppressed AD symptoms and high serum IgE levels. CJLP55 administration reduced the thickness of the epidermis, infiltration of mast cells and eosinophils into the skin lesion, enlargement of axillary lymph nodes, and increase in cell population in axillary lymph nodes. CJLP55 treatment decreased the production of type 2 cytokines, such as interleukin (IL)-4, IL-5, IL-10, IL-12, interferon (IFN)-${\gamma}$, and IL-6,which were stimulated by house dust mite extracts, in the axillary lymph node cells. Orally administered CJLP55 exhibited a therapeutic effect on house dust mite-induced AD in NC/Nga mice after onset of the disease by altering immune cell activation. The Lactobacillus strain, CJLP55, isolated from Kimchi, suppressed AD. Our results suggest its possible use as a potential candidate for management of AD.
Atopic dermatitis (AD) is an inflammatory skin condition accompanied by symptoms such as edema and hemorrhage. Kimchi is a traditional fermented Korean dish consisting of various probiotics. In this study, the therapeutic effect of Lactobacillus plantarum CJLP55 isolated from Kimchi was studied in AD-induced mice. Orally administered Lactobacillus strain, CJLP55, suppressed AD symptoms and high serum IgE levels. CJLP55 administration reduced the thickness of the epidermis, infiltration of mast cells and eosinophils into the skin lesion, enlargement of axillary lymph nodes, and increase in cell population in axillary lymph nodes. CJLP55 treatment decreased the production of type 2 cytokines, such as interleukin (IL)-4, IL-5, IL-10, IL-12, interferon (IFN)-${\gamma}$, and IL-6,which were stimulated by house dust mite extracts, in the axillary lymph node cells. Orally administered CJLP55 exhibited a therapeutic effect on house dust mite-induced AD in NC/Nga mice after onset of the disease by altering immune cell activation. The Lactobacillus strain, CJLP55, isolated from Kimchi, suppressed AD. Our results suggest its possible use as a potential candidate for management of AD.
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가설 설정
Although it is very meaningful to identify the lactic acid bacteria that have a preventive effect on AD, they do not show the effect on patients already suffering from AD. Based on the questions that arose from a prior study [9], in this study, we investigated whether the administration of CJLP55 can treat AD after its onset. To examine the therapeutic effect of CJLP55, AD was primarily induced for three weeks in NC/Nga mice; later, CJLP55 strain was administered in mice for eight weeks.
These previous studies focused on the preventive effect of lactic acid bacteria on AD because the induction of AD was performed after the administration of Lactobacillus spp. Therefore, we wanted to study if CJLP55 has a therapeutic effect on AD induced by Dfb extract along with its preventive effect. AD was preferentially induced for 21 d and CJLP55 was administered in mice for the next 56 d.
제안 방법
After the administration of lactic acid bacteria for 56 d, H&E staining was conducted on the dorsal skin lesion. The thickness of the dermis was measured to evaluate the progress of lichenification. The mice in the control group showed epidermal hyperplasia, but it was not observed in mice in the non-induction group (Fig.
To determine whether orally administered CJLP55 affects the production of various cytokines, cells isolated from the axillary lymph nodes were cultured with Dfb extract for 48 h, and then, the concentration of cytokines in the supernatant was analysed. Levels of Th2 cytokines, such as IL-4 and IL-5, produced by the lymph node cells were increased in AD-induced mice in control group without Lactobacillus administration (Fig.
대상 데이터
Lactobacillus plantarum CJLP55 (KCTC 11401BP) was obtained from CJ Foods R&D Center, CJ CheilJedang Corporation (Suwon-si, Gyeonggi-do, Korea).
데이터처리
Statistical interpretation was conducted by Student’s t-test.
성능/효과
3A and 3B). Consistent with the results of dermatitis symptoms, these results showed that dexamethasone effectively regulated the mast cell infiltration during induction of AD, and CJLP55 suppressed the accumulation of the mast cells in the dorsal skin lesion in a dose dependent manner, excluding CJLP55-5. Unlike previous result of CJLP55-HK, heat-killed CJLP55 administration did not show any effect on infiltration of mast cells.
In conclusion, orally administered viable and heat-killed CJLP55 suppressed the skin inflammation and AD-like skin lesions, and altered the immune cell populations and activation. These results suggest that CJLP55 may have a therapeutic potential in the treatment of AD.
Increased IL-6 production and the difference of IL-6 genotype was observed in patients with AD [28,29], but most studies involving AD do not confirm the effect on IL-6, maybe because of its unexplained mechanisms. In this study, IL-6 production from the lymph node cells was increased in mice in the control group, and CJLP55 suppresses it in a dose dependent manner. This result suggests the need to further study about the role and regulation of IL-6 in AD.
All CJLP55-administered groups without CJLP55-5 group, displayed a great recovery in a dose dependent manner after day 42. On the final day of experiment, CJLP55-9 and CJLP55-10 administration reduced the dermatitis score to more than half of that of control, and the difference in the effect was not observed for 109 and 1010 CFU/mouse group. Administration of CJLP55-7 clearly diminished the manifestation of dermatitis, but showed only half of the effect than that of CJLP55-9 and CJLP55-10.
In our study, 1010 CFU of live CJLP55 were heat-killed and administered in mice. The mice in this experimental group showed an improvement in AD, which was similar to the effect of 107 CFU of live lactobacilli-administered group. These results indicate that heat-killed CJLP55 also has a therapeutic effect due to its component, but it causes a reduced effect as compared to that of live microbes.
In order to elucidate whether the four selected strains affect the induction of AD, the symptoms and immune responses were studied in mice with AD induced by the topical application of house dust mite Dermatophagoides farinae body (Dfb) extract followed by administration of the strains [9]. Two of the four selected strains, CJLP55 and CJLP133, showed a definite effect on immune response modulation during the induction of AD. Although it is very meaningful to identify the lactic acid bacteria that have a preventive effect on AD, they do not show the effect on patients already suffering from AD.
후속연구
In this study, IL-6 production from the lymph node cells was increased in mice in the control group, and CJLP55 suppresses it in a dose dependent manner. This result suggests the need to further study about the role and regulation of IL-6 in AD.
참고문헌 (30)
1 Bieber T Atopic dermatitis Ann Dermatol 2010 22 125 137 20548901
3 Rodriguez JM Murphy K Stanton C Ross RP Kober OI Juge N Avershina E Rudi K Narbad A Jenmalm MC Marchesi JR Collado MC The composition of the gut microbiota throughout life, with an emphasis on early life Microb Ecol Health Dis 2015 26 26050 25651996
4 West CE Jenmalm MC Prescott SL The gut microbiota and its role in the development of allergic disease: a wider perspective Clin Exp Allergy 2015 45 43 53 24773202
5 Hill C Guarner F Reid G Gibson GR Merenstein DJ Pot B6 Morelli L Canani RB Flint HJ Salminen S Calder PC Sanders ME Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic Nat Rev Gastroenterol Hepatol 2014 11 506 514 24912386
6 Schiavi E Barletta B Butteroni C Corinti S Boirivant M Di Felice G Oral therapeutic administration of a probiotic mixture suppresses established Th2 responses and systemic anaphylaxis in a murine model of food allergy Allergy 2011 66 499 508 21058959
7 Matsuzaki T Takagi A Ikemura H Matsuguchi T Yokokura T Intestinal microflora: probiotics and autoimmunity J Nutr 2007 137 3 Suppl 2 798S 802S 17311978
8 Won TJ Kim B Song DS Lim YT Oh ES Lee DI Park ES Min H Park SY Hwang KW Modulation of Th1/Th2 balance by Lactobacillus strains isolated from Kimchi via stimulation of macrophage cell line J774A.1 in vitro J Food Sci 2011 76 H55 H61 21535768
9 Won TJ Kim B Lim YT Song DS Park SY Park ES Lee DI Hwang KW Oral administration of Lactobacillus strains from Kimchi inhibits atopic dermatitis in NC/Nga mice J Appl Microbiol 2011 110 1195 1202 21338447
11 Matricardi PM Bjorksten B Bonini S Bousquet J Djukanovic R Dreborg S Gereda J Malling HJ Popov T Raz E Renz H Wold A EAACI Task Force 7 Microbial products in allergy prevention and therapy Allergy 2003 58 461 471 12757444
13 Segawa S Hayashi A Nakakita Y Kaneda H Watari J Yasui H Oral administration of heat-killed Lactobacillus brevis SBC8803 ameliorates the development of dermatitis and inhibits immunoglobulin E production in atopic dermatitis model NC/Nga mice Biol Pharm Bull 2008 31 884 889 18451512
15 Sunada Y Nakamura S Kamei C Effect of Lactobacillus acidophilus strain L-55 on the development of atopic dermatitis-like skin lesions in NC/Nga mice Int Immunopharmacol 2008 8 1761 1766 18790088
16 Matsuda H Watanabe N Geba GP Sperl J Tsudzuki M Hiroi J Matsumoto M Ushio H Saito S Askenase PW Ra C Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice Int Immunol 1997 9 461 466 9088984
18 Lim HS Ha H Lee MY Jin SE Jeong SJ Jeon WY Shin NR Sok DE Shin HK Saussurea lappa alleviates inflammatory chemokine production in HaCaT cells and house dust mite-induced atopic-like dermatitis in Nc/Nga mice Food Chem Toxicol 2014 63 212 220 24216625
19 Matsuoka H Maki N Yoshida S Arai M Wang J Oikawa Y Ikeda T Hirota N Nakagawa H Ishii A A mouse model of the atopic eczema/dermatitis syndrome by repeated application of a crude extract of house-dust mite Dermatophagoides farinae Allergy 2003 58 139 145 12622745
20 Yamamoto M Haruna T Yasui K Takahashi H Iduhara M Takaki S Deguchi M Arimura A A novel atopic dermatitis model induced by topical application with dermatophagoides farinae extract in NC/Nga mice Allergol Int 2007 56 139 148 17460441
21 Imai Y Yasuda K Sakaguchi Y Haneda T Mizutani H Yoshimoto T Nakanishi K Yamanishi K Skin-specific expression of IL-33 activates group 2 innate lymphoid cells and elicits atopic dermatitis-like inflammation in mice Proc Natl Acad Sci U S A 2013 110 13921 13926 23918359
22 Staumont-Salle D Fleury S Lazzari A Molendi-Coste O Hornez N Lavogiez C Kanda A Wartelle J Fries A Pennino D Mionnet C Prawitt J Bouchaert E Delaporte E Glaichenhaus N Staels B Julia V Dombrowicz D CX 3 CL1 (fractalkine) and its receptor CX 3 CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin J Exp Med 2014 211 1185 1196 24821910
23 Kapp A The role of eosinophils in the pathogenesis of atopic dermatitis--eosinophil granule proteins as markers of disease activity Allergy 1993 48 1 5
25 Laouini D Alenius H Bryce P Oettgen H Tsitsikov E Geha RS IL-10 is critical for Th2 responses in a murine model of allergic dermatitis J Clin Invest 2003 112 1058 1066 14523043
27 Grewe M Bruijnzeel-Koomen CA Schopf E Thepen T Langeveld-Wildschut AG Ruzicka T Krutmann J A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis Immunol Today 1998 19 359 361 9709503
28 Toshitani A Ansel JC Chan SC Li SH Hanifin JM Increased interleukin 6 production by T cells derived from patients with atopic dermatitis J Invest Dermatol 1993 100 299 304 8440909
29 Gharagozlou M Farhadi E Khaledi M Behniafard N Sotoudeh S Salari R Darabi B Fathi SM Mahmoudi M Aghamohammadi A Amirzargar AA Rezaei N Association between the interleukin 6 genotype at position -174 and atopic dermatitis J Investig Allergol Clin Immunol 2013 23 89 93
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