Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restr...
Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restricted to 50% of the entire composition. Therefore, to use H. abdominalis as a food ingredient, H. abdominalis has to be prepared as a mixture by adding other materials. In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. In addition, the anti-fatigue activity of a mixture comprising H. abdominalis and red ginseng (RG) was evaluated to commercially utilize H. abdominalis in food industry. H. abdominalis was hydrolyzed using Alcalase, a protease, and the effect of H. abdominalis hydrolysate (HH) on the muscles was assessed in C2C12 myoblasts by measuring cell proliferation and glycogen content. In addition, the mixtures comprising HH and RG were prepared at different percentages of RG to HH (20, 30, 40, 50, 60, 70, and 80% RG), and the anti-fatigue activity of these mixtures against oxidative stress was assessed in C2C12 myoblasts. In C2C12 myoblasts, $H_2O_2$-induced oxidative stress caused a decrease in viability and physical fatigue-related biomarkers such as glycogen and ATP contents. However, treatment with RG and HH mixtures increased cell viability and the content of fatigue-related biomarkers. In particular, the 80% RG mixture showed an optimum effect on cell viability and ATP synthesis activity. In this study, all results indicated that HH had anti-fatigue activity at concentrations approved for use in food by the law in Korea. Especially, an 80% RG to HH mixture can be used in food for ameliorating fatigue.
Seahorse, a syngnathidae fish, is one of the important organisms used in Chinese traditional medicine. Hippocampus abdominalis, a seahorse species successfully cultured in Korea, was validated for use in food by the Ministry of Food and Drug Safety in February 2016; however. the validation was restricted to 50% of the entire composition. Therefore, to use H. abdominalis as a food ingredient, H. abdominalis has to be prepared as a mixture by adding other materials. In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. In addition, the anti-fatigue activity of a mixture comprising H. abdominalis and red ginseng (RG) was evaluated to commercially utilize H. abdominalis in food industry. H. abdominalis was hydrolyzed using Alcalase, a protease, and the effect of H. abdominalis hydrolysate (HH) on the muscles was assessed in C2C12 myoblasts by measuring cell proliferation and glycogen content. In addition, the mixtures comprising HH and RG were prepared at different percentages of RG to HH (20, 30, 40, 50, 60, 70, and 80% RG), and the anti-fatigue activity of these mixtures against oxidative stress was assessed in C2C12 myoblasts. In C2C12 myoblasts, $H_2O_2$-induced oxidative stress caused a decrease in viability and physical fatigue-related biomarkers such as glycogen and ATP contents. However, treatment with RG and HH mixtures increased cell viability and the content of fatigue-related biomarkers. In particular, the 80% RG mixture showed an optimum effect on cell viability and ATP synthesis activity. In this study, all results indicated that HH had anti-fatigue activity at concentrations approved for use in food by the law in Korea. Especially, an 80% RG to HH mixture can be used in food for ameliorating fatigue.
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문제 정의
In this study, the effect of H. abdominalis on muscles was investigated to scientifically verify its potential bioactivity. Also, the anti-fatigue activity of a mixture comprising H.
In this study, the effect of H. abdominalis on the muscles was investigated to scientifically verify its potential bioactivity. Also, the anti-fatigue activity of a mixture comprising HH and RG was evaluated to commercially utilize H.
SYK contributed to conduct the cell experiments and analyze the biomarker levels. SR managed aquaculture of the seahorse and supported it for this study. JYK contributed to design the study and conduct the experiments.
2013). These studies showed values of in vitro study to evaluate the potential anti-fatigue activity through in vivo study. In the present study, the mixtures of HH and RG acted as the antioxidant for hydrogen peroxide and showed the anti-fatigue activity on C2C12 myoblast.
제안 방법
NK contributed to conduct the research and prepare the draft manuscript. SYK contributed to conduct the cell experiments and analyze the biomarker levels. SR managed aquaculture of the seahorse and supported it for this study.
abdominalis in the food market. The anti-fatigue activity of H. abdominalis and a mixture was evaluated by measuring the levels of physical fatigue-related biomarkers such as serum glycogen and ATP contents.
2016). Thus, H. abdominalis mixtures were prepared by adding different concentrations of RG (20, 30, 40, 50, 60, 70, and 80% of RG) to investigate the synergy effect between H. abdominalis and RG on anti-fatigue activity.
대상 데이터
The cytotoxicity was assessed by MTT assay. The experiment was performed in triplicate. Each value indicates the mean ± standard error from three independent experiments
데이터처리
The results were subjected to analysis of variance using Tukey’s test to analyze the differences.
이론/모형
Effect of these mixtures on cell viability was estimated using the MTT assay. The viabilities of C2C12 myoblasts treated with the mixtures at different concentrations (50, 100, 200, 250, and 500 μg/mL) were expressed to represent over 90% viability, which was similar to that of the control cells (Fig.
The cell was treated with various concentrations of seahorse hydrolysate (50, 100, 150, and 200 μg/mL) and incubated for 72 h. The cell proliferation was assessed by BrdU assay. The experiment was performed in triplicate.
The cell was treated with various concentrations of seahorse hydrolysate (50, 100, 150, and 200 μg/mL) and incubated for 24 h. The cytotoxicity was assessed by MTT assay. The experiment was performed in triplicate.
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