This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, ...
This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were $8{\mu}g/mL$ for Streptococcus mutans, and $16{\mu}g/mL$ for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration $8-16{\mu}g/mL$. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.
This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were $8{\mu}g/mL$ for Streptococcus mutans, and $16{\mu}g/mL$ for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration $8-16{\mu}g/mL$. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.
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제안 방법
The stained cells were rinsed twice with 300 μL of PBS, fol-lowed by solubilizing the cells in 200 μL of 95% ethanol, and the degree of biofilm formation was determined by measuring the OD575 with a spectrophotometer.
데이터처리
The results were analyzed by the mixed model procedure of SAS® version 9.2 (SAS Institute, USA).
2 (SAS Institute, USA). Mean comparisons for the fixed effect were performed with the pairwise t-test, and significance was determined at alpha = 0.05.
이론/모형
The minimum inhibitory concentration (MIC) of Psoraleae semen extract on bacterial growth was determined using the two-fold broth micro-dilution method (NCCLS, 1997). Briefly, each bacterial strain was subcultured and was grown to mid-logarithmic growth phase in BHI broth.
성능/효과
In addition, antimicrobial activities of 69 herbal plant extracts on various pathogens including Gram-positive and Gram-negative bacteria were tested using a disc diffusion assay. Among them, only five extracts (Rubi fructus, Galla rhois, Moutan xortex Radicis, Sanguisorba officinalis Linne, and Psoraleae semen) were effective for inhibiting more than three different bacteria (Table 1). Rubi fructus inhibited growth of all bacterial strains tested in this study.
aureus compared to the other bacteria. Second, structural difference of the bacteria probably causes discrepant outcome. S.
In conclusions, Rubi fructus, Galla rhois, Moutan xortex Radicis, Sanguisorba officinalis Linne, and Psoraleae semen have antimicrobial activity against various pathogenic bacteria. Among them, Psoraleae semen extract could be used as a natural antimicrobial to control the growth of Gram-positive bacteria including Enterococci, S.
후속연구
, 2005). Thus, further study is necessary to find appropriate conditions and procedure to use Psoralea semen extract as food antimicrobial.
For example, Gram-negative bacteria have outer membranes, which may prevent the extract used in this study from exerting its antimicrobial activity. However, further study is necessary to elucidate the antimicrobial mechanisms of this extract against Gram-negative bacteria.
참고문헌 (25)
1. Bereksi N. Gavini F. Benezech T. Faille C. Growth, morphology and surface properties of Listeria monocytogenes Scott A and LO28 under saline and acid environments J. Appl. Microbiol. 2002 92 556 565 10.1046/j.1365-2672.2002.01564.x 11872133
2. Fani M. M. Kohanteb J. Dayaghi M. Inhibitory activity of garlic (Allum sativum) extract on multidrug-resistant Streptococcus mutans J. Indian Soc. Pedod. Prev. Dent. 2007 25 164 168 10.4103/0970-4388.37011 18007101
3. Guliani A. Pirri G. Nicoletto S. F. Antimicrobial peptides: An overview of a promising class of therapeutics Cent. Eur. J. Biol. 2007 2 1 33
4. Henie E. F. P. Zaiton H. Suhaila M. Bacterial membrane disruption in food pathogens by Psidium guajava leaf extracts Int. Food Res. J. 2009 16 297 311
5. Hood S. K. Zottola E. A. Bioflms in food processing Food Control 1995 6 9 18 10.1016/0956-7135(95)91449-U
6. Hwang J. H. Rha S. J. Cho J. K. Shin T. S. Kim M. Y. Kim J. D. Psoraleae semen extract inhibits angiogenesis and adipogenesis Biotechnol. Bioproc. Eng. 2013 18 942 949 10.1007/s12257-013-0379-0
7. Jackson A. L. Kulchaiyawat C. Sullivan G. A. Sebranek J. G. Dickson J. S. Use of natural ingredients to control growth of Clostridium perfringens in naturally cured frankfurters and hams J. Food. Prot. 2011 74 417 424 10.4315/0362-028X.JFP-10-365 21375878
8. Katsura H. Tsukiyama R. I. Suzuki A. Kobayashi M. In vitro antimicrobial activities of bakuchiol against oral microorganisms Antimicrob. Agents Chemother. 2001 45 3009 3013 10.1128/AAC.45.11.3009-3013.2001 11600349
9. Kaya I. Yigit N. Benli M. Antimicrobial activity of various extracts of Ocimum Basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy Aft. J. Trad. CAM. 2008 5 363 369
10. Kuete V. Wabo G. F. Ngameni B. Mbaveng A. T. Metuno R. Etoa F. X. Ngadjui B. T. Beng V. P. Meyer J. J. M. Lall N. Antimicrobial activity of the methanolic extract, fractions and compounds from the stem bark of Irvinia gabonensis (Ixonanthaceae) J. Ethnopharmacol. 2007 114 54 60 10.1016/j.jep.2007.07.025 17766070
11. Leela T. Satirapipathkul C. Studies on the antibacterial activity of quercus infectoria galls Int. Con. Biosci. Biochem. Bioinform. (IPCBEE) 2011 5 410 414
12. Liu C. S. Cham T. M. Yan C. H. Chang H. W. Chen C. H. Chuan L. Y. Antibacterial properties of Chinese herbal medicines against nosocomial antibiotic resistant strains of Pseudomonas aeruginosa in Taiwan Am. J. Chinese Med. 2007 35 1047 1060 10.1142/S0192415X07005508
14. Loo C. Y. Corliss D. A. Ganeshkumar N. Streptococcus gordonii biofilm formation: identification of genes that code for biofilm phenotypes J. Bacteriol. 2000 182 1374 1382 10.1128/JB.182.5.1374-1382.2000 10671461
15. Navarre W. W. Schneewind O. Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope Microbiol. Mol. Biol. Rev. 1999 63 174 229 10066836
16. NCCLS (National Committee for Clinical Llaboratory Standards) Performance standards for antimicrobial disk susceptibility tests: Approved standard M2-A7 Wayne: PA: USA 1997
17. Plesiat P. Nikaido H. Outer membranes of gram-negative bacteria are permeable to steroid probes Mol. Microbiol. 1992 6 1323 1333 10.1111/j.1365-2958.1992.tb00853.x 1640833
18. Shim S. H. Kim Y. Lee J. Y. Song D. G. Aldose reductase inhibitory activity of the compounds from the seed of Psoralea corylifolia J. Korean Soc. Appl. Biol. Chem. 2009 52 568 572 10.3839/jksabc.2009.096
19. Wong R. W. K. Rabiem A. B. M. Effect of Buguzhi (Psoralea corylifolia fruit) extract on bone formation Phytother. Res. 2010 24 155 160 10.1002/ptr.3049
20. Wu F. Wang A. F. Zhou Y. Zhou T. S. Jiang H. L. Fang Y. Z. Determination of psoralen and isopsoralen in traditional Chinese medicines by capillary zone electrophoresis with amperometric detection Chromatographia 2005 61 157 160 10.1365/s10337-004-0476-5
21. Xiong Z. Wang D. Wu Y. Li F. Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L Int. J. Phamaceutic. Sci. 2003 58 925 928
22. Yim N. H. Jung Y. P. Cho W. K Kim T. Kim A. Im M. Ma J. Y. Screening of aqueous extracts of medicinal herbs for antimicrobial activity against oral bacteria J. Integr. Med. 2013 2 18 24
23. Yoon Y. Choi K. H. Identification of inhibitory effect on Streptococcus mutans by oleanolic acid J. Life Sci. 2010 3 321 325
24. Yoon Y. Choi K. H. Antimicrobial activities of therapeutic herbal plants against Listeria monocytogenes and the herbal plant cytotoxicity on Caco-2 cell Lett. Appl. Microbiol. 2012 55 47 55 10.1111/j.1472-765X.2012.03262.x 22563627
25. Zaide S. F. Yamada K. Kadowaki M. Usmanghani K. Sugiyama T. Bactericidal activity of medicinal plants, employed for the treatment of gastrointestinal ailments, against Helicobacter pylori J. Ethnopharmacol. 2009 121 286 291 10.1016/j.jep.2008.11.001 19041711
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