[논문]방사선에 조사된 쥐에서 아로니아의 방사선 방호효과

문환식; 이준행

doi:10.17946/jrst.2017.40.3.10

방사선에 조사된 쥐에서 아로니아의 방사선 방호효과
Radioprotective Effects of Aronia on Radiation Irradiated Rats 원문보기

방사선기술과학 = Journal of radiological science and technology, v.40 no.3, 2017년, pp.423 - 431

초록
AI-Helper

본 연구에서는 아로니아를 Rat에 경구 투여 후 방사선을 1회 전신 조사한 다음 혈액검사를 실시해 백혈구, 적혈구, 혈소판 등의 혈구세포의 변화 상태를 관찰하고 비교 분석하여 아로니아의 방사선 방어효과를 알아보고자 하였다. 실험동물은 SD계 Rat 6주령 200~250g 수컷 15마리를 이용하여 5마리를 하나의 군으로 정상군 (A), 5 Gy 대조군(B), 5 Gy 실험군(C)으로 설정하였고, 정상군(A)은 방사선 조사를 실시하지 않고, 대조군 (B)은 일반식 이를 투여하여 방사선조사를 하였고, 실험군(C)은 방사선조사 전 14일 동안 아로니아, 증류수 경구투여량(100 mg/kg/day)로 1일 2회씩 50 mg/kg/day로 각각 쥐에 14일 동안 경구 투여 후 방사선 조사(500 cGy/min로 5 Gy)를 하였다. 그 결과 정상군(A), 대조군(B), 실험군(C)에 따른 백혈구, 적혈구, 혈소판 차이를 비교해 본 결과 통계적으로 유의한 차이가 없었지만, 세부적으로 살펴보면, 림프구, 혈색소, 평균적혈구색소량에서 모두 정상군(A)이 대조군(B), 실험군(C)에 비해서 평균수치가 높게 나타나 통계적으로 유의한 차이가 있음을 알 수 있었다. 림프구에서는 정상군(A)과 대조군(B), 정상군(A)과 실험군(C)간에 통계적으로 유의한 차이가 보였으며, 평균적 혈구색소량에서는 정상군(A)과 실험군(C)에서 통계적으로 유의한 차이를 보였다. 본 연구의 결과로 보아 방사선에 조사된 쥐에서 아로니아의 방사선방호효과가 전체적으로 백혈구, 적혈구, 혈소판 등은 없는 것으로 사료되었고, 부분적으로는 림프구, 혈색소, 평균적혈구혈색소량은 통계적 유의성이 있는 것으로 알 수 있었다. 이 실험을 바탕으로 향후 다양한 연구가 선행되어야 할 것으로 사료된다.

Abstract ▼ AI-Helper

The present study was intended to orally administer aronia to rats, irradiate radiation once to the whole bodies of the rats, and conduct blood tests to observe, compare, and analyze changes in blood cells, such as leukocytes, erythrocytes, and platelets, in order to examine the radioprotective effects of aronia. As experimental animals, 15 male Sprague-Dawley (SD) rats aged six weeks weighing 200~250 g were taken and divided into the normal group (A) of five rats, the 5 Gy control group (B) of five rats, and the 5 Gy experimental group (C) of five rats. The normal group (A) was not~irradiated at all, the control group (B) was administered with general diets and irradiated, and the experimental group(C) was orally administered with 50 mg/kg/day of aronia two times per day to achieve a distilled water oral dose of 100 mg/kg/day and irradiated thereafter (5 Gy at 500 cGy/min) for 14 days. After the experiment, differences in leukocytes, erythrocytes, and platelets among the normal group (A), the control group (B), and the experimental group (C) were examined by comparing the counts of the blood cells and the results showed no statistically significant differences. However, on a detailed review, the normal group (A) showed statistically higher mean values for all of lymphocytes, hemoglobin, and mean corpuscular hemoglobin as compared to the control group (B) and the experimental group (C). Statistically significant differences in the counts of lymphocytes were shown between the normal group (A) and the control group (B), and between the normal group (A) and the experimental group (C); furthermore, statistically significant differences in mean corpuscular hemoglobin were shown between the normal group (A) and the experimental group (C). Given the results of the present study, in irradiated rats, aronia was generally considered as having no radioprotective effect on leukocyte, erythrocyte, and platelet while having statistically significant radioprotective effects on lymphocytes, hemoglobin, and mean corpuscular hemoglobin. Based on the present experiment, diverse studies should be conducted hereafter.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

The purpose of the present study was to orally administer aronia to rats, irradiate radiation once to the whole bodies of the rats, and conduct blood tests to observe, compare, and analyze changes in blood cells such as leukocytes, erythrocytes, and platelets in order to examine the radioprotective effects of aronia.
The temperature and humidity of the breeding room were maintained at 23±2°d and 50∼60%, respectively. The stainless wire covered plastic cages were subjected to 12 hour light-darkness cycles shifting at 09:00 and 21:00. Each group of rats consisting of five rats was bred in each cage; feed and water were freely supplied.
Second, the rats were under severe stress before being irradiated and this seemed to have resulted in poor results and the same stress had to be imposed on individual rats. Third, the experimental period had to be divided into three and the rats had to be irradiated on day 7, 14, and 30 instead of setting the experimental period as 14 days. In future studies, the number of rats per experimental group should be increased, the same stress should be imposed on the animals before irradiation, and the experimental period should be longer.
This study conducted an experiment to see “whether red ginseng extracts can be used as a radioprotective substance” and reported that red ginseng extracts had components with antioxidative activity[15].
To examine the radioprotective effects of aronia in irradiated rats, a total of 15 rats irradiated with 5 Gy radiation on the whole bodies using a Linac 6 MegaVoltage (MV) X-ray therapeutic apparatus (CLINAC 21 IX model) were divided into the normal group (A), the control group (B), and the experimental group (C) consisting of five rats each and blood tests were conducted. To review cell susceptibility according to Bergonie-Tribondeau's law, susceptibility to radiation was higher among cells with larger neogenesis abilities, those with longer processes of cell division, those with lower degrees of morphological and functional differentiation, and those that are younger and weaker.
When the results of the present experiment were examined in detail to compare and analyze the leukocytes in the control group and the experimental group. Based on the results, the mean values of the control group were lower than those of other groups.

대상 데이터

Each group of rats consisting of five rats was bred in each cage; feed and water were freely supplied. The experiment was conducted when the rats were healthy after passing an adaptation period of seven days immediately before the experiment and the animal laboratory of the Department of Food Engineering of Chonnam University was used as a breeding room.
The experimental animals were divided into the normal group (A) of five rats, the 5 Gy control group (B) of five rats, and the 5 Gy experimental group (C) of five rats. The normal group (A) was not irradiated at all, the control group (B) was administered with general diets and irradiated, and the experimental group (C) was orally administered with 50 mg/kg/day of aronia two times per day to achieve a distilled water oral dose of 100 mg/kg/day using zondes for rats for 14 days before irradiation.

데이터처리

Since the data did not satisfy the normality based on the test of normality, the data were analyzed using the Kruskal-Wallis test method which is a non-parametric method and Mann-Whitney tests were used as ex post facto tests (significance level α=0.05).

성능/효과

First, since the number of rats per the experimental group was too small, accurate and reliable data could not be easily produced. Second, the rats were under severe stress before being irradiated and this seemed to have resulted in poor results and the same stress had to be imposed on individual rats. Third, the experimental period had to be divided into three and the rats had to be irradiated on day 7, 14, and 30 instead of setting the experimental period as 14 days.
On a detailed review, it could be seen that the normal group showed higher mean values for all of lymphocytes, hemoglobin, and mean corpuscular hemoglobin as compared to other groups. Statistically significant differences in the counts of lymphocytes were shown between the normal group and the control group, and between the normal group and the experimental group, while statistically significant differences in mean corpuscular hemoglobin were observed between the normal group and the experimental group. On reviewing other elements, it could be seen that the mean counts of neutrophilic leukocytes, monocytes among leukocytes, eosinophilic leukocytes, and basophilic leukocytes in the normal group were lower as compared to other groups.

참고문헌 (17)

Kim, S. H., Oh, H., Lee, S. E., et al., "Radioprotective Effect of Red Ginseng in Irradiated Mice with High and Low Dose of Radiation", Korean Journal of Ginseng Society, Vol. 22, No. 1, pp. 66-72, 1998.
Hall, E. J., Radiobiology for the radiologist, 4th ed., J. B. Lippincott Company, 1994.
Radiation Biology Research Society., Radiobiology, CHUNG-KU Publisher, pp. 122, 2015.
Chae, K. M., Choi, K. H., Kim, Y. H., et al., "Radioprotective Effect of Panax Ginseng in Mouse Bone-marrow", Journal Korean Asso, Radiation Protection, Vol. 22, No. 1, pp. 1-7, 1997.
Ku, Y. H., Lee, J. H., Ji, T. J., et al., Radiobiology, JUNG-MUN-KAG Publisher, pp. 60, 2015.
Jeppsson, N., & Johansson, R., "Changes in fruit quality in black chokeberry (Aronia melanocarpa) during maturation", J Hort Sci Biotechnol 73: pp. 340-345, 2000.
Tsuneo, T., & Akira, T., "Chemical components and characteristics of black chokeberry", Nippon Shokuhin Kagaku Kogaku Kaishi 48: pp. 606-610, 2001.

상세보기
Jeong, J. M., "Antioxidative and Antiallergic Effects of Aronia (Aronia melanocarpa) Extract", Journal the Korean Society food science nutrition, Vol. 37, No. 9, pp. 1109-1113, 2008.

원문보기 상세보기
Yamamoto, M., Hayashi, Y., Ohshima, H., et al., "Effect of ginsenoside on DNA, protein and lipid synthesis in bone marrow", Symposia for WAKAN YAKU (in Japaness), Vol. 6, pp. 49-54, 1972.
Yu, J. I., & Lee, J. H., "Protective Effects of Water dropwort and Jin-do turmeric on the Irradiation in Mouse". Journal of the Korean Society of Radiology, Vol. 5, No. 6, pp. 335-341, 2011.

원문보기 상세보기
Seung, K. Y., Lee, J. H., Kim, J. S., et al., "Radioprotective Effect of Red Ginseng in Irradiated Mice with ${\gamma}$ -ray", Journal of the Korean Society of Radiology, Vol. 4, No. 1, pp. 31-35, 2010.
Stewart, C. B., RADIOLGIC SCIENCE for TECHNOLOGISTS, 10th ed., JMK, pp. 476-480. 2016.
Kim, J. S., "Protective Effects of Chitosanoligos accharide on the Irradiation of Gamma-ray in Mice", Ph.D. thesis, Chosun University, 2003.
Jin, G. H., Min, S. Y., Lee, J. H., et al., "The Effects of Rat's Sperm Bioassay for Low Dose X-RAY", Journal of Radiation Protection, Vol. 32, No. 4, pp. 184-189, 2007.
Kim, D. J., & Chang, C. C., "The Effects of Red Ginseng Extracts on Activities and Lipid Peroxidation of the Kidney in ${\gamma}$ -Postirradiated Mice", Korean Journal of Ginseng Society. Vol. 18, No. 1, pp. 25-31. 1994.
Kim, J. 0., Choi, J. H., Shin, J. H., et al., "The Effects of Cynanchi wilfordii Radix Ethanol Extracts upon Irradiated Rat's Blood and Organ", Journal of Korean Radiological Science and Technology, Vol. 39, No. 3, pp. 451-459. 2016.

원문보기 상세보기
Ji, T. J., "A Study on Radio-Protection Mechanism of Platelet Cells After Injection of Alliin", Journal of Korean Radiological Science and Technology, Vol. 33, No. 3, pp. 185-191. 2010.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증