Kwon, O-Chul
(Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration)
,
Ju, Wan-Taek
(Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration)
,
Kim, Hyun-Bok
(Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration)
,
Sung, Gyoo-Byung
(Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration)
,
Kim, Yong-Soon
(Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, Rural Development Administration)
BACKGROUND: Spoilage fungi can reduce the shelf life of fresh fruits and cause economic losses by lowering quality. Especially, mulberry fruits have high sensitivity to fungal attack due to their high water content (> 70%) and soft texture. In addition, the surface of these fruits is prone to damage...
BACKGROUND: Spoilage fungi can reduce the shelf life of fresh fruits and cause economic losses by lowering quality. Especially, mulberry fruits have high sensitivity to fungal attack due to their high water content (> 70%) and soft texture. In addition, the surface of these fruits is prone to damage during harvesting and postharvest handling. However, any study on postharvest spoilage fungi in mulberry fruit has not been reported in Korea. This study aimed to examine the spoilage fungi occurring in mulberry fruits during storage after harvest. METHODS AND RESULTS: In this study, we isolated postharvest spoilage fungi from mulberry fruits stored in refrigerator (fresh fruits) and deep-freezer (frozen fruits) and identified them. In the phylogenetic analysis based on comparisons of the ITS rDNA sequences, the 18 spoilage fungi isolated from mulberry fruits and the 25 reference sequences were largely divided into seven groups that were subsequently verified by high bootstrap analysis of 73 to 100. Alternaria spp. including A. alternate and A. tenuissima, were the most frequently isolated fungi among the spoilage isolates: its occurrence was the highest among the 18 isolates (38.9%). CONCLUSION: The findings of this study will be helpful for increasing the shelf life of mulberry fruits through the application of appropriate control measures against infection by spoilage fungi during storage.
BACKGROUND: Spoilage fungi can reduce the shelf life of fresh fruits and cause economic losses by lowering quality. Especially, mulberry fruits have high sensitivity to fungal attack due to their high water content (> 70%) and soft texture. In addition, the surface of these fruits is prone to damage during harvesting and postharvest handling. However, any study on postharvest spoilage fungi in mulberry fruit has not been reported in Korea. This study aimed to examine the spoilage fungi occurring in mulberry fruits during storage after harvest. METHODS AND RESULTS: In this study, we isolated postharvest spoilage fungi from mulberry fruits stored in refrigerator (fresh fruits) and deep-freezer (frozen fruits) and identified them. In the phylogenetic analysis based on comparisons of the ITS rDNA sequences, the 18 spoilage fungi isolated from mulberry fruits and the 25 reference sequences were largely divided into seven groups that were subsequently verified by high bootstrap analysis of 73 to 100. Alternaria spp. including A. alternate and A. tenuissima, were the most frequently isolated fungi among the spoilage isolates: its occurrence was the highest among the 18 isolates (38.9%). CONCLUSION: The findings of this study will be helpful for increasing the shelf life of mulberry fruits through the application of appropriate control measures against infection by spoilage fungi during storage.
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문제 정의
However, little is known about the spoilage fungi of postharvest mulberry fruits in Korea, and there is a lack of proper control measures. Thus, this study could provide valuable basic information that will facilitate the increase of the shelf life of mulberry fruits through appropriate control measures against infection from spoilage fungi during storage.
제안 방법
Five hundred grams of mulberry fruit samples were randomly collected from four different mulberry varieties (Cheong-Il, Gwasang 2, Su Hyang, and Shim Heung) in the Sericulture and Apicuture Division of the Department of Agricultural Biology, Rural Development Administration, Jeon-Ju, Republic of Korea. After harvest, the samples were placed in separated sterile plastic containers and transferred to the laboratory, where they were kept separately in a refrigerator (fresh fruits) and deep-freezer (frozen fruits) for four days and two weeks, respectively, until further analysis. To isolate spoilage fungi, the fruits were rinsed with sterile water and dried at room temperature.
대상 데이터
Group 1 included seven isolates (A1, A2, A3, A4, A5, A6, and E1-2), three Alternaria alternata, (reference sequence CQ-WF-A2, H7, and PAA) and three A. tenuissima (reference sequence CS09, GS-BH-A2, and YK12) isolates. The ITS rDNA sequences of isolates A1, A2, A4, A5, A6, and E1-2 showed 100% identity with three A.
이론/모형
The cultured fungi were next ground to a fine powder using liquid nitrogen. Genomic DNA was extracted using the CTAB method (Cao et al., 1998). Then, the extracted DNA was purified by phenol-chloroform-isoamyl alcohol (25:24:1) and precipitated with one volume of isopropanol.
html). The phylogenetic tree was constructed using MEGA7 program (Kumar et al., 2016) based on the neighbor-joining (NJ) method (Saitou and Nei 1987). The confidence levels for the individual branches of the resulting tree were assessed by the bootstrap test (Felsenstein, 1985), in which 1,000 replicate trees were generated from resampled data.
성능/효과
The ITS rDNA sequences of isolates A1, A2, A4, A5, A6, and E1-2 showed 100% identity with three A. alternata (reference sequence CQ-WFA2, H7, and PAA), whereas that of the isolate A3 displayed 99.7%–100% identity with the three of A. tenuissima (reference sequence CS09, GS-BH-A2, and YK12).
The PCR product sizes of the ITS rDNA regions were of variable length, from 534 to 608 bp. Trichoderma atroviride had the longest ITS rDNA region (607 and 608 bp), whereas Phoma herbarum had the shortest one (534 bp). In the phylogenetic analysis, the 18 spoilage fungi isolated from mulberry fruits and 25 reference sequences were largely divided into seven groups (Fig.
참고문헌 (41)
International Journal of Agriculture and Biology N., Akhtar 15 6 1283 (2013)
African Journal of Microbiology Research R. R., Al-Hindi 5 4 443 (20110
Saudi Journal of Biological Sciences S. S., Alwakeel 20 4 311 (2013) 10.1016/j.sjbs.2013.05.002
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