선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- SYH and SCK designed this study and drafted the manuscript. WKJ conceived and designed the study and also revised the manuscript.
- The PCR was performed using selective primers for the iNOS (5′-ATGTC CGAAGCAAACATC AC-3′ and 5′-TAATGTCCAGGAA GTAGGTG-3′), CO X-2 (5′-GGGGTACCTTCCAGCTGTCAAAA TCTC-3′ and 5′-GAAGATCTCGCCAGGTACTCACCTG-3′), TN F-α (5′-ATGAGCACAGAAAGCATGA TC-3′ and 5′- TACAGGCTTGTCACTCGAATT-3′), IL-1β (5′-ATGGCAACTGTTCCTGAACTCAACT-3′ and 5′-TTTCCTT TCTTAGATATGGACAGGAC-3′), IL-6 (5′-ATGAGCA CAGAAAGCATGATC-3′ and 5′-TACAGGCTTGTCAC TCGAATT-3′), and GAPDH (5′-TTTGTGATGGGTGT GAACCACGAG-3′ and 5′-GGAGACAACCTGGTCCT CAGTGTA-3′).
- The signals were developed using an ECL Western blotting detection kit and quantified using Davinci K chemi-doc imaging system (Young Hwa scientific Co. LTD., Seoul, Korea), and protein expression was quantified by Image J software (Wayne Rasband, NIH, Bethesda, Md., USA).
- 2010). Therefore, the present study was investigated to evaluate the anti-inflammatory effects of J. belengerii frame hydrolysate (JFH) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, as well as the mechanism underlying this effect.
- To evaluate whether the JFH suppressed the production of LPS-stimulated NO, RAW 264.7 macrophages were stimulated with LPS in the absence or presence of the JFH. The result shows that LPS treatment alone markedly induced NO production by the treated cells compared with untreated cells.
- SYH and SCK designed this study and drafted the manuscript. WKJ conceived and designed the study and also revised the manuscript. All authors read and approved the final manuscript.
대상 데이터
- Pepsin from porcine gastric mucosa, 3- (4, 5–dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) Griess reagent, and LPS were purchased from Sigma Aldrich (Sigma Aldrich, St. Louis, USA).
데이터처리
- Statistical comparisons of the mean values were performed by analysis of variance (ANOVA), followed by Duncan’s multiple range test using SPSS software.
이론/모형
- MTT assay was performed to assess the cytotoxicity of RAW 264.7 macrophages. Briefly, cells were seeded on the plate and culture with various concentrations of the JFH (10, 50, and 100 μg/ml) for 24 h.
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