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제안 방법
- In this study, we aimed to investigate the anti-inflammatory effect of 70% ethanol extracts from the root of Hibiscus syriacus, and to elucidate the potential mechanism associated with its anti-inflammatory activity.
- PCR was carried out using PCR Master Mix Kit (Promega, Madison, WI, USA) with mouse primers for iNOS, IL-1β and GAPDH as followed : mouse iNOS: forward 5’-ttgtgcatcgacctaggctggaa-3’ and reverse 5’-gacctttcgcattagca tggaagc-3’, mouse IL-1β: forward 5’-ggcaggcagtatcactcatt-3’ and reverse 5’-cccaaggccacaggtattt-3’, GAPDH: forward 5’-ggactgtggtcatgagcccttcca-3’ and reverse 5’-actcacggcaaattc aacggcac-3’.
- 7 cells. To exclude that inhibition of NO production by RHS-E70 results from the reduction of the cell viability, we evaluated the effect of RHS-E70 on the viability of RAW264.7 cells. As shown in Fig.
대상 데이터
- Dulbecco’s Modified Eagle medium (DMEM)/F-12 1:1 Modified medium (DMEM/F-12) for the cell culture was purchased from Lonza (Walkersville, MD, USA).
- Dulbecco’s Modified Eagle medium (DMEM)/F-12 1:1 Modified medium (DMEM/F-12) for the cell culture was purchased from Lonza (Walkersville, MD, USA). Lipopolysaccharide (LPS) was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibodies against IκB-α, phospho-IκB-α, p65, phospho-ERK1/2, ERK1/2, phospho-p38, p38, and β-actin were purchased from Cell Signaling (Bervely, MA, USA).
데이터처리
- Statistical analysis was performed with one-way ANOVA followed by Dunnett's test.
이론/모형
- Cell viability was evaluated by MTT assay. Briefly, cells were plated at a density of 3 × 104 cells/well in 96-well plate and incubated for 24 h.
- 7 cells were pretreated with RHS-E70 for 6 h and then co-treated with LPS (1 ㎍/㎖) for 18 h. The determination of NO production was measured by Griess assay. (B) RAW264.
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