[논문]화장품 천연원료로서 옥수수잎 추출물의 효능 비교연구

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doi:10.14400/jdc.2018.16.9.513

초록
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국내에서 재배되는 옥수수의 수확 후 버려지는 잎을 수거하여 음건하고 분쇄 한 후 실험에 사용하였다. 두 가지 용매로 추출 후 DPPH, ABTS, 총 플라보노이드 함량, 총 폴리페놀 함량을 측정하여 항산화 활성을 분석하고, Paper disc diffusion method 로 clear zone의 직경을 측정 하였다. 그 결과 에탄올 추출물이 모든 항산화활성과 항균활성에서 다소 높은 결과를 나타내었다. Gallic acid를 표준물질로 하여 총 polyphenol 함량을 측정한 결과 CE는 $31.2{\pm}5.2mg\;GAE/g$로 높은 활성을 보였고, DPPH radical 소거능 측정 결과 CE는 1, 10, 100, 1,000 (${\mu}g/mL$) 농도에서 각각 $4.6{\pm}0.9%$, $6.4{\pm}0.5%$, $22.9{\pm}0.6%$, $83.2{\pm}0.2%$로 나타나 농도 의존적 경향을 보였으며 1,000 (${\mu}g/mL$)에서는 매우 높은 활성을 보였다. 지금까지 옥수수와 옥수수 수염에 대한 연구는 많지만 페기 되는 잎에 대한 연구는 없어 본 연구가 새로운 천연 항산화, 항균 효능을 가진 원료개발의 기초 자료가 될 것으로 판단된다.

Abstract ▼ AI-Helper

In this study, Zea mays L., which were discarded after harvesting in Korea, were collected and used for experiment. The Zea mays L. were dried in the shade and ground before being extracted using two solvents. Subsequently, DPPH, ABTS, the total flavonoid content and the total polyphenol content wer...

In this study, Zea mays L., which were discarded after harvesting in Korea, were collected and used for experiment. The Zea mays L. were dried in the shade and ground before being extracted using two solvents. Subsequently, DPPH, ABTS, the total flavonoid content and the total polyphenol content were measured to analyze antioxidant activity. The diameter of clear zone was measured by using the paper disc diffusion method. The results showed that the ethanol extract had a slightly higher antioxidant and antibacterial activity. The total polyphenol content was measured using gallic acid as standard, and CE(ethanol extract) was found to contain $31.2{\pm}5.2mg\;GAE/g$ of polyphenols, indicating a considerably high activity. When DPPH radical scavenging ability was measured, CE was found to have $4.6{\pm}0.9%$, $6.4{\pm}0.5%$, $22.9{\pm}0.6%$ and $83.2{\pm}0.2%$ at different concentrations of $1{\mu}g/mL$, $10{\mu}g/mL$, $100{\mu}g/mL$, and $1,000{\mu}g/mL$, respectively, with a dependent tendency at the concentration of $1,000{\mu}g/mL$ and a considerably high activity at the concentration of $1,000{\mu}g/mL$. This study is expected to be used as preliminary data to develop new natural antioxidant, antibacteria raw materials, as only little research has been undertaken on Zea mays L., which are mostly discarded after harvesting, while many studies have been so far conducted on corn and corn silks.

주제어

표/그림 (5)

표 Table 1. Total Polyphenol Contents of CD and CE
그림 Fig. 1. DPPH radical scavenging activity of CD and CE. CD and CE were incubated at 1, 10, 100, and 1,000 ㎍/mL with DPPH solution for 30 mins. Activities were determined by measurement of absorbance at 517 ㎚. The results were expressed as mean ± S.D from three independent experiments.
표 Table 2. Total Flavonoid Contents of CD and CE
그림 Fig. 2. ABTS radical scavenging activity of CD and CE. CD and CE were incubated at 1, 10, 100, and 1,000 ㎍/mL with ABTS solution for 10 mins. Activities were determined by measurement of absorbance at 732 nm. The results were expressed as mean ± S.D from three independent experiments.
표 Table 3. Determination of antimicrobial activity Growth inhibition size of clear zone

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문제 정의

This study aimed to identify the possibility that corn leaf extract can be used as natural functional cosmetic material. Corn leaves was extracted using water and ethanol, respectively, and the antioxidant activity and antibacterial activity were compared and reviewed.

제안 방법

Various studies on corn silk and stalk have been conducted, however, only little research on corn leaf has been carried out. In this regard this study intended to evaluate the possibility that corn leaf, which is discarded in bulk every year, can be used as natural cosmetic materials by analyzing its antioxidant and antibacterial activity.

대상 데이터

The corn leaves used in this experiment were obtained after harvesting corn in Jecheon, Korea. They were ground and dried to contain less than or equal to40% moisture to produce the corn leaf distilled water extract (CD) and the corn leaf ethanol extract (CE).
The four strains released from Korea Research Institute of Bioscience & Biotechnology Propionibacterium acnes (P. acnes 3314), Staphylococcus aureus(S.aureus) gram-positive bacteria and Pseudomonasaeruginosa(P .aeruginosa), Escherichia coli(E. coli)gram-negative bacteria.

데이터처리

The results of the experiment were processed statistically using unpaired student's T-test in SPSS18.0 and ANOVA.

성능/효과

The ABTS radical scavenging activity of CD andCE was 0.5 ± 0.1%, 2.2 ± 0.2%, 3.0 ± 0.6%, and 79.3 ±0.8% at 1, 10, 100 and 1,000 ㎍/mL respectively Theconcentration of CE was 0.7 ± 0.2%, 3.1 ± 0.9%, 5.4 ± 0.5%,and 89.4 ± 0.4% at concentrations of 1, 10, 100 and 1,000μg/mL Curl scavenging ability increased (see Fig. 2).
The DPPH radical scavenging activity of CD and CEwas measured as 3.6 ± 0.6%, 6.7 ± 0.8%, 21.9 ± 0.4%and 78.2 ± 0.5% at the concentrations of 1, 10, 100 and1,000 ㎍ /mL , And CE was 4.6 ± 0.9%, 6.4 ± 0.5%, 22.9± 0.6%, and 83.2 ± 0.2% at concentrations of 1, 10, 100,and 1,000 μg / mL, respectively.
Corn leaves was extracted using water and ethanol, respectively, and the antioxidant activity and antibacterial activity were compared and reviewed. The results showed that the corn leaf ethanol extract (CE)had higher antioxidant activity and antibacterial activity to some extent than the corn leaf distilled water extract (CD). The total polyphenol content was measured by using gallic acid as standard, and CE was found to contain 31.

후속연구

However, few studies have been undertaken on the use of corn leaves, which are mostly discarded after harvesting. The results of this study suggest the possibility that corn leaf extract may be used as cosmetic materials with antioxidant activity.

참고문헌 (15)

S. M. Lim. (2009). Study on the revitalization of cosmetic museum according to the development of cosmetics industry. Master of Thesis. Dongkuk University, Seoul.
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H. J. Jeon & H. J. Kwon. (2016). Anti-inflammation Effect of Gynura Procumbens extract. Journal of Digital Convergence, 14(10), 515-520.
S. J. Shin et al. (2010). Relationship between biomass components dissolution and enzymatic saccharification of several ammonium hydroxide soaked biomasses. Journal of Korea Technical Association of The Pulp and Paper Industry, 42(1), 35-40.
J. T. Kim et al. (2013). Natural Dyeing Fabrics with Leaf and Stem of Purple Corn. Korean Journal of Crop Science, 58(2), 113-118.

원문보기 상세보기
N. R. Woo et al. (2005). Antioxidative and Antimicrobial Activities of Extracts from Different Parts of Crotalaria sessiflora L. Journal of the Korean Society of Food Science and Nutrition, 34(7), 948-952.

원문보기 상세보기
E. Y. Hwang, Y. H. Kong, Y. C. Lee, Y. C. Kim, K. M. Yoo, Y. O. Jo & S. Y. Choi. (2006). Comparison of phenolic compound contents between white and red ginseng and their inhibitory effect on melanin biosynthesis. J. Ginseng Res, 30(1), 82-87.

원문보기 상세보기
M. K. Kang, S. K. Kim & Y. H. Kang. (2009). Antioxidant Activity and Functional Components of Corn Silk (Zea mays L.). Korean J. Plant Res,. 22(4), 323-329.
H. Y. Kim et al. (2016). Antioxidative and Protective Effects of Corn Silk (Zea mays L.) Extract on Human HaCaT Keratinocyte. Korean J. Crop Sci, 61(3), 184-190.

원문보기 상세보기
J. C. Ju, J. H. Shin, S. J. Lee, H. S. Cho & N. J. Sung. (2006). Antioxidative Activity of Hot Water Extracts from Medicinal Plants. Journal of the Korean Society of Food Science and Nutrition, 35(1), 7-14.

원문보기 상세보기
H. B. Lee, B. Y. Park, H. C. Ji, J. W. Cho & S. H. Kim. (2006). Antioxidant Activity and Agronomic Characteristics of Colored Waxy Corns. Korean journal of crop science, 51(1), 179-184.
Fossen, T., R. & Slimestad, O. M. (2001). Andersen, Anthocyanins from maize (Zea mays) and reed canarygrass (Phalaris arundinacea). J. Agric. Food Chem, 49(1), 2318-2321.

상세보기
J. Y. Park, J. S. Jin, H. Y. Kang, E. H. Jeong, J. C. Lee, Y. C. Lee, S. Y. Seol, D. T. Cho & J. Kim. (2007). A comparison of adult and pediatric methicillin-resistant Staphylococcus aureus isolates collected from patients at a university hospital. J. of the Microbiol, 45(5), 447-452.
S. H. Eom, J. H. Park, D. U. Yu, J. L. Choi, J. D. Choi, M. S. Lee & Y.M. Kim. (2011). Antimicrobial activity of brown alga Eisenia bicyclis against methicillin-resistant Staphylococcus aureus. J. of the ish Aquat. Sci, 14(4), 251-256.
S. J. Wang, H. J. Lee, J. Y. Cho, K. H. Park & J. H. Moon. (2012). Isolation and Identification of Antioxidants from Makgeolli. Korean J Food Sci Technol, 44(1), 14-20.

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화장품 천연원료로서 옥수수잎 추출물의 효능 비교연구
A Comparative Study on the Efficacy of Zea mays L. Extracts as a Natural Ingredient in Cosmetics 원문보기

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화장품 천연원료로서 옥수수잎 추출물의 효능 비교연구 A Comparative Study on the Efficacy of Zea mays L. Extracts as a Natural Ingredient in Cosmetics 원문보기

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화장품 천연원료로서 옥수수잎 추출물의 효능 비교연구
A Comparative Study on the Efficacy of Zea mays L. Extracts as a Natural Ingredient in Cosmetics 원문보기

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