본 연구에서는 천연 화장품 소재 개발을 위하여 접시꽃 캘러스 생리활성 효능에 대해서 평가하였다. 접시꽃캘러스 추출물의 항산화 효능은 DPPH, ABTS, FRAP assay를 통해서 확인하였다. 그 결과 접시꽃캘러스 추출물은 농도 의존적으로 강력한 항산화 능력을 확인되었다. 더불어, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 세포 내 ROS를 효과적으로 감소시키는 것으로 확인되었다. 접시꽃 캘러스 추출물의 미백효능을 평가하기 위해 tyrosinase 저해 효과를 확인한 결과, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 tyrosinase 활성을 51% 감소시키는 것으로 확인되었다. 이상의 결과를 바탕으로, 접시꽃 캘러스 추출물은 항산화 및 미백효능을 지닌 화장품 기능성 소재로서 적용 가치가 높은 천연소재로 사료된다.
본 연구에서는 천연 화장품 소재 개발을 위하여 접시꽃 캘러스 생리활성 효능에 대해서 평가하였다. 접시꽃캘러스 추출물의 항산화 효능은 DPPH, ABTS, FRAP assay를 통해서 확인하였다. 그 결과 접시꽃캘러스 추출물은 농도 의존적으로 강력한 항산화 능력을 확인되었다. 더불어, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 세포 내 ROS를 효과적으로 감소시키는 것으로 확인되었다. 접시꽃 캘러스 추출물의 미백효능을 평가하기 위해 tyrosinase 저해 효과를 확인한 결과, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 tyrosinase 활성을 51% 감소시키는 것으로 확인되었다. 이상의 결과를 바탕으로, 접시꽃 캘러스 추출물은 항산화 및 미백효능을 지닌 화장품 기능성 소재로서 적용 가치가 높은 천연소재로 사료된다.
In this study, we investigated the biological effects of Alcea rosea L. callus extract for the development of natural cosmetics ingredients. The antioxidant activities of A. rosea L. callus extract was measured through DPPH, ABTS and FRAP assay. As a result, A. rosea L. callus extract were found to ...
In this study, we investigated the biological effects of Alcea rosea L. callus extract for the development of natural cosmetics ingredients. The antioxidant activities of A. rosea L. callus extract was measured through DPPH, ABTS and FRAP assay. As a result, A. rosea L. callus extract were found to have a strong antioxidant ability in a dose dependent manner. In addition, A. rosea L. callus effectively reduced the intracellular oxidative stress induced by AAPH at a concentration of 10 mg/mL. In a tyrosinase activity assay, we found that A. rosea L. callus extract reduced tyrosinase activity by 51% at 10 mg/mL. Based on these results, A. rosea L. callus extract is considered as a promising natural ingredients for cosmetics with antioxidant and whitening functions.
In this study, we investigated the biological effects of Alcea rosea L. callus extract for the development of natural cosmetics ingredients. The antioxidant activities of A. rosea L. callus extract was measured through DPPH, ABTS and FRAP assay. As a result, A. rosea L. callus extract were found to have a strong antioxidant ability in a dose dependent manner. In addition, A. rosea L. callus effectively reduced the intracellular oxidative stress induced by AAPH at a concentration of 10 mg/mL. In a tyrosinase activity assay, we found that A. rosea L. callus extract reduced tyrosinase activity by 51% at 10 mg/mL. Based on these results, A. rosea L. callus extract is considered as a promising natural ingredients for cosmetics with antioxidant and whitening functions.
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제안 방법
callus extract affects cell viabilities on human normal skin cell lines. Crystal violet staining assay and MTT assay were performed to investigate whether A. rosea L. callus extract is toxic on HaCaT, HDF, CCD-986sk (Figure 2A, B). We found that the A.
The antioxidant effect of A. rosea L. callus extract was evaluated by measuring DPPH, ABTS radial scavenging activities.
대상 데이터
TRP1 (sc-514900), Tyrosinase (sc-7833), β-actin (sc-47778), was purchased from Santa Cruz Biotechnology (USA).
데이터처리
For two experimental comparisons, data were analyzed using the unpaired Student’s t-test. For multiple comparisons, data were analyzed using one-way ANOVA with Tukey posttest. Values represent means ± SD of three independent experiments performed in triplicate; *p < 0.
For two experimental comparisons, data were analyzed using the unpaired Student’s t-test.
이론/모형
. Cell viability was evaluated using cell proliferation kit I (Applied Science, Germany) and crystal violet staining method. Cells into 96-well plates at densities of 1 × 105 cells well-1, washed twice with phosphate buffer saline (PBS).
성능/효과
callus extract also affects melanogenesis related protein expressions in α-MSH stimulated B16F10 cells. Micropthalmia-associated transcription factor (MITF), Tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1) expression were evaluated. MITF, Tyrosinase, TRP-1 expressions are strongly upregulated after α-MSH treatment.
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