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NTIS 바로가기대한영양사협회 학술지 = Journal of the Korean dietetic association, v.24 no.4, 2018년, pp.330 - 343
이지연 (단국대학교 식품영양학과) , 임혜지 (단국대학교 식품영양학과) , 김미숙 (단국대학교 식품영양학과)
Recently, there has been a growing demand for natural preservatives because of increased consumer interest in health. In this study, we produced Lactobacillus rhamnosus cell-free supernatant (LCFS) and evaluated and compared its antimicrobial activity with existing natural preservatives against path...
핵심어 | 질문 | 논문에서 추출한 답변 |
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라이소자임의 항균활성에 대한 한계점은? | 치즈, 와인, 생면 등에 보존료로 널리 사용되고 있는 라이소자임은 달걀 흰자에 함유된 효소로 미생물 세포벽인 펩티도글리칸의 N-acetylglucosamine과 N-acetylmuramic acid의 결합을 끊어 항균 활성을 나타낸다(Cho 등 2005). 그러나 상대적으로 펩티도글리칸층이 두꺼운 그람양성균에서만 항균 효과가 뛰어나다고 보고되고 있다(Gill & Holley 2003). 식품보존료로 이용되고 있는 유기산은 젖산, 초산, 구연산 등 여러 종류가 있으며, 식품의 원료에 발효산물로 존재하거나 인공적으로 첨가하고 있다(Chang 등 2008). | |
천연 보존료 대체제로 가능성이 있는 L. rhamnosus 배양물의 라이소자임 활성 및 유기산 함유량은? | aureus를 각각 오염시킨 닭 가슴살에서 이들 균의 증식에 미치는 영향을 알아보았다. LCFS는 30 Unit/mg protein의 라이소자임 활성을 보유하였고, 젖산 18,835mg/L, 아세트산 5,060 mg/L, 시트르산 2,051 mg/L을 함유하였으며 항균 활성은 온도 50∼95°C와 pH 3∼9에서 유지되었다. LCFS는 자몽종자추출물, Durafresh, egg white lysozyme(EWL)보다 E. | |
라이소자임이 항균 활성을 나타내는 방법은? | 자몽 종자추출물의 경우 미생물의 세포막을 약화시킴으로 인해 세포 증식을 억제하여 항균 효과가 있다고 보고되고 있으나, 이는 자몽종자추출물에서 잔류 용매에 의한 항균 효과라는 보고도 발표되고 있어 안전성에 문제가 제기되고 있다(Ha 등 2009). 치즈, 와인, 생면 등에 보존료로 널리 사용되고 있는 라이소자임은 달걀 흰자에 함유된 효소로 미생물 세포벽인 펩티도글리칸의 N-acetylglucosamine과 N-acetylmuramic acid의 결합을 끊어 항균 활성을 나타낸다(Cho 등 2005). 그러나 상대적으로 펩티도글리칸층이 두꺼운 그람양성균에서만 항균 효과가 뛰어나다고 보고되고 있다(Gill & Holley 2003). |
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