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대한영양사협회 학술지 = Journal of the Korean dietetic association, v.24 no.4, 2018년, pp.330 - 343
이지연 (단국대학교 식품영양학과) , 임혜지 (단국대학교 식품영양학과) , 김미숙 (단국대학교 식품영양학과)
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Recently, there has been a growing demand for natural preservatives because of increased consumer interest in health. In this study, we produced Lactobacillus rhamnosus cell-free supernatant (LCFS) and evaluated and compared its antimicrobial activity with existing natural preservatives against path...
| 핵심어 | 질문 | 논문에서 추출한 답변 |
|---|---|---|
| 라이소자임의 항균활성에 대한 한계점은? | 치즈, 와인, 생면 등에 보존료로 널리 사용되고 있는 라이소자임은 달걀 흰자에 함유된 효소로 미생물 세포벽인 펩티도글리칸의 N-acetylglucosamine과 N-acetylmuramic acid의 결합을 끊어 항균 활성을 나타낸다(Cho 등 2005). 그러나 상대적으로 펩티도글리칸층이 두꺼운 그람양성균에서만 항균 효과가 뛰어나다고 보고되고 있다(Gill & Holley 2003). 식품보존료로 이용되고 있는 유기산은 젖산, 초산, 구연산 등 여러 종류가 있으며, 식품의 원료에 발효산물로 존재하거나 인공적으로 첨가하고 있다(Chang 등 2008). | |
| 천연 보존료 대체제로 가능성이 있는 L. rhamnosus 배양물의 라이소자임 활성 및 유기산 함유량은? | aureus를 각각 오염시킨 닭 가슴살에서 이들 균의 증식에 미치는 영향을 알아보았다. LCFS는 30 Unit/mg protein의 라이소자임 활성을 보유하였고, 젖산 18,835mg/L, 아세트산 5,060 mg/L, 시트르산 2,051 mg/L을 함유하였으며 항균 활성은 온도 50∼95°C와 pH 3∼9에서 유지되었다. LCFS는 자몽종자추출물, Durafresh, egg white lysozyme(EWL)보다 E. | |
| 라이소자임이 항균 활성을 나타내는 방법은? | 자몽 종자추출물의 경우 미생물의 세포막을 약화시킴으로 인해 세포 증식을 억제하여 항균 효과가 있다고 보고되고 있으나, 이는 자몽종자추출물에서 잔류 용매에 의한 항균 효과라는 보고도 발표되고 있어 안전성에 문제가 제기되고 있다(Ha 등 2009). 치즈, 와인, 생면 등에 보존료로 널리 사용되고 있는 라이소자임은 달걀 흰자에 함유된 효소로 미생물 세포벽인 펩티도글리칸의 N-acetylglucosamine과 N-acetylmuramic acid의 결합을 끊어 항균 활성을 나타낸다(Cho 등 2005). 그러나 상대적으로 펩티도글리칸층이 두꺼운 그람양성균에서만 항균 효과가 뛰어나다고 보고되고 있다(Gill & Holley 2003). |
Andani HRR, Tukmechi A, Meshkini S, Sheikhzadeh N (2012): Antagonistic activity of two potential probiotic bacteria from fish intestines and investigation of their effects on growth performance and immune response in rainbow trout (Oncorhynchus mykiss). J Appl Ichthyol 28(5):728-734
Barefoot SF, Chen YR, Hughes TA, Bodine AB, Shearer MY, Hughes MD (1994): Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B. Appl Environ Microbiol 60(10):3522-3528
Benachour A, Ladjouzi R, Le Jeune A, Hebert L, Thorpe S, Courtin P, Chapot-Chartier MP, Prajsnar TK, Foster SJ, Mesnage S (2012): The lysozyme-induced peptidoglycan N-acetylglucosamine deacetylase PgdA (EF1843) is required for Enterococcus faecalis virulence. J Bacteriol 194(22):6066-6073
Beristain-Bauza SC, Mani-Lopez E, Palou E, Lopez-Malo A (2016): Antimicrobial activity and physical properties of protein films added with cell-free supernatant of Lactobacillus rhamnosus. Food Control 62:44-51
Chang HT, Cheng YH, Wu CL, Chang ST, Chang TT, Su YC (2008): Antifungal activity of essential oil and its constituents from Calocedrus macrolepis var. formosana Florin leaf against plant pathogenic fungi. Bioresour Technol 99(14):6266-6270
Chin KB, Kim WY, Kim KH (2005): Physicochemical and textural properties, and antimicrobial effects of low-fat comminuted sausages manufactured with grapefruit seed extract. Korean J Food Sci Anim Resour 25(2):141-148
Cho MH, Bae EK, Ha SD, Park JY (2005): Application of natural antimicrobials to food industry. Food Sci Ind 38(2):36-45
Chouliara E, Karatapanis A, Savvaidis IN, Kontominas MG (2007): Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at 4 degrees C. Food Microbiol 24(6):607-617
Chun JW, Ma CW, Oh KH (2005): Physiological characterization of Lactobacillus sp. JK-8 isolated from shrimpaquaculture pond. Korean J Microbiology 41(1):18-23
Conner DE, Scott VN, Bernard DT (1990): Growth, inhibition, and survival of Listeria monocytogenes as affected by acidic conditions. J Food Prot 53(8):652-655
Coyette J, Shockman GD (1973): Some properties of the autolytic N-acetylmuramidase of Lactobacillus acidophilus. J Bacteriol 114(1):34-41
Cvetnic Z, Vladimir-Knezevic S (2004): Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharm 54(3):243-250
Daeschel MA, McKenney MC, McDonald LC (1990): Bacteriocidal activity of Lactobacillus plantarum C-11. Food Microbiol 7(2):91-98
De Carvalho KG, Bambirra FH, Kruger MF, Barbosa MS, Oliveira JS, Santos AM, Nicoli JR, Bemquerer MP, de Miranda A, Salvucci EJ, Sesma FJ, Franco BD (2010): Antimicrobial compounds produced by Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product. J Ind Microbiol Biotechnol 37(4):381-390
De Keersmaecker SC, Verhoeven, TL, Desair J, Marchal K, Vanderleyden J, Nagy I (2006): Strong antimicrobial activity of Lactobacillus rhamnosus GG against Salmonella typhimurium is due to accumulation of lactic acid. FEMS Microbiol Lett 259(1):89-96
Ganzle MG (2004): Reutericyclin: biological activity, mode of action, and potential applications. Appl Microbiol Biotechnol 64(3):326-332
Ganzle MG, Holtzel A, Walter J, Jung G, Hammes WP (2000): Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584. Appl Environ Microbiol 66(10):4325-4333
Gill AO, Holley RA (2003): Interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, nisin and EDTA in the presence of nitrite and sodium chloride at 24 degrees C. Int J Food Microbiol 80(3):251-259
Hao YY, Brackett RE, Doyle MP (1998): Efficacy of plant extracts in inhibiting Aeromonas hydrophila and Listeria monocytogenes in refrigerated, cooked poultry. Food Microbiol 15(4):367-378
Kamei K, Hara S, Ikenaka T, Murao S (1988): Amino acid sequence of a lysozyme (B-enzyme) from Bacillus subtilis YT-25. J Biochem 104(5):832-836
Kim CR (2001): Microbiological and sensory evaluations of refrigerated chicken in summer. J Fd Hyg Saf 16(1):16-20
Kim CR, Koh DH, Kim YJ, Kim KH, Choi IK, Eun JB (1999): Microbiological evaluations of retail and refrigerated chickens in winter. Prev Nutr Food Sci 12(2): 109-112
Kim MR (1997): Effect of lactic acid treatment on microorganisms and sensory characteristics in chickens. Korean J Soc Food Cook Sci 13(3):293-298
Kong YJ, Park BK, Oh DH (2001): Antimicrobial activity of quercus mongolica leaf ethanol extract and organic acids against food-borne microorganisms. Korean J Food Sci Technol 33(2):178-183
Kullisaar T, Songisepp E, Mikelsaar M, Zilmer K, Vihalemm T, Zilmer M (2003): Antioxidative probiotic fermented goats' milk decreases oxidative stress-mediated atherogenicity in human subjects. Br J Nutr 90(2):449-456
Kullisaar T, Zilmer M, Mikelsaar M, Vihalemm T, Annuk H, Kairane C, Kilk A (2002): Two antioxidative lactobacilli strains as promising probiotics. Int J Food Microbiol 72(3):215-224
Kwon NH, Kim SH, Bae WK, Kim JK, Lim JY, Noh KM, Kim JM, Ann JS, Huh J, Park YH (2001): Antimicrobial activity of Lactobacillus reuteri against major food-borne pathogens. Korean Soc Food Hyg Saf 16(4):264-273
Lee JW, Hong SI, Son SM, Chang YH (2003): Characterization of antimicrobial polymeric films for food packaging applications. J Korean Soc Appl Biol Chem 10(4):574-583
Lee JY, Park YS, Kim YS, Shin DH (2002): Antimicrobial characteristics of metabolites of lactic acid bacteria isolated from feces of newborn baby and from Dongchimi. Korean J Food Sci Technol 34(3):472-479
Leer RJ, van der Vossen JM, van Giezen M, Van Noort JM, Pouwels PH (1995): Genetic analysis of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus. Microbiology 141(Pt 7):1629-1635
Miao J, Guo H, Ou Y, Liu G, Fang X, Liao Z, Ke C, Chen Y, Zhao L, Cao Y (2014): Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp. tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China. Food Control 42:48-53
Miao J, Peng W, Liu G, Chen Y, Chen F, Cao Y (2015): Biopreservative effect of the natural antimicrobial substance from Lactobacillus paracasei subsp. tolerans FX-6 on fresh pork during chilled storage. Food Control 56:53-56
Oh MG (2015): Antibacterial characteristics of cell-free supernatants of lysozyme producing bacteria and the mixture of lysozyme and organic acids. Ph.D thesis. Dankook University. pp. 17
Park HK, Kim SB (2006): Antimicrobial activity of grapefruit seed extract. Korean J Food Nutr 19(4):526-531
Pirarat N, Pinpimai K, Endo M, Katagiri T, Ponpornpisit A, Chansue N, Maita M (2011): Modulation of intestinal morphology and immunity in nile tilapia (Oreochromis niloticus) by Lactobacillus rhamnosus GG. Res Vet Sci 91(3):e92-97
Platas G, Meseguer I, Amils R (2002): Purification and biological characterization of halocin H1 from Haloferax mediterranei M2a. Int Microbiol 5(1):15-19
Rammelsberg M, Radler F (1990): Antibacterial polypeptides of Lactobacillus species. J Appl Bacteriol 69(2):177-184
Russell SM (1997): Rapid prediction of the potential shelf-life of fresh broiler chicken carcasses under commercial conditions. J Appl Poult Res 6(2):163-168
Sarika AR, Lipton AP, Aishwarya MS (2010): Bacteriocin production by a new isolate of Lactobacillus rhamnosus GP1 under different culture conditions. Adv J Food Sci Technol 2(5):291-297
Silva M, Jacobus NV, Deneke C, Gorbach SL (1987): Antimicrobial substance from a human Lactobacillus strain. Antimicrob Agents Chemother 31(8):1231-1233
Sowani HM, Thorat P (2012): Antimicrobial activity studies of bactoriocin produced by Lactobacilli isolates from carrot kanji. OnLine J Biol Sci 12(1):6-10
Taniguchi M, Nakazawa H, Takeda O, Kaneko T, Hoshino K, Tanaka T (1998): Production of a mixture of antimicrobial organic acids from lactose by co-culture of Bifidobacterium longum and Propionibacterium freudenreichii. Biosci Biotechnol Biochem 62(8):1522-1527
Tolinacki M, Kojic M, Lozo J, Terzic-Vidojevic A, Topisirovic L, Fira D (2010): Characterization of the bacteriocin-producing strain Lactobacillus paracasei subsp. Paracasei BGUB9. Arch Biol Sci 62(4):889-899
Varsha KK, Nampoothiri KM (2016): Appraisal of lactic acid bacteria as protective cultures. Food Control 69:61-64
Vermeiren L, Devlieghere F, Van Beest M, de Kruijf N, Debevere J (1999): Developments in the active packaging of foods. Trends Food Sci Technol 10(3):77-86
Yoon SY, Lee SY, Kim KBWR, Song EJ, Lee SJ, Lee CJ, Park NB, Jung JY, Kawk JH, Nam KW, Ahn DH (2010): Antimicrobial activity of the Sargassum fulvellum ethanol extract and the effect of temperature and pH on their activity. Korean J Food Sci Technol 42(2):155-159
Zhang H, Wu J, Guo X (2016): Effects of antimicrobial and antioxidant activities of spice extracts on raw chicken meat quality. Food Sci Hum Wellness 5(1):39-48
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