[논문]전신과민성 식품 알레르기 마우스 모델에서의 도두(刀豆) 추출물의 항염 효과

양원경; 박양춘; 김한영; 김근회; 노성수; 김승형

doi:10.6116/kjh.2018.34.1.1.

전신과민성 식품 알레르기 마우스 모델에서의 도두(刀豆) 추출물의 항염 효과
Anti-inflammatory Effects of Canavaliae Semen(Canavalia gladiate) Extracts in a Systemic Anaphylaxis Food Allergy Mouse Model 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.34 no.1, 2019년, pp.1 - 11

양원경 (대전대학교 한의과대학 폐계내과학교실) , 박양춘 (대전대학교 한의과대학 폐계내과학교실) , 김한영 (애경중앙연구소) , 김근회 (애경중앙연구소) , 노성수 (대구한의대학교 한의과대학) , 김승형 (대전대학교 동서생명과학연구원)

Abstract ▼ AI-Helper

Objective : An allergy to peanuts is a major cause of fatal food-induced anaphylaxis, with food allergies becoming an increasingly important health research issue. Food allergy as clinical entity has been recongnized for many years, although there is yet no general concord as to the incidence of this symptom.1) Methods : This study was undertaken to verify the effect of seeds of Canavalia gladiata (Jacq.) DC. extract (CGE) on the inhibition of allergic reactions using a cholera toxin and peanut extract-immunized food allergy mouse model. We determine whether the changes in rectal temperature were related to energy consumption owing to heat production in the body. Mast cell distribution and degranulation in the dermis and epidermis were observed with an optical microscope. Subsequently, Ara h1 levels in serum and interleukin (IL)-4, IL-10, and $IFN-{\gamma}$ levels in cultured supernatants of splenocytes were measured. Results : CGE treatment significantly attenuated the secretion of the Ara h1 antibody in serum and splenocytes. Ara h 1 was undetected in the cholera toxin and peanut extract-immunized food allergy mouse model. Improvement in ear tissue inflammation symptoms was the CGE experimental group. In the control group and peanut extract control group, the expression of mast cells was higher, whereas that in the CGE experimental group was significantly lower. Conclusion : CGE causes suppression in a food allergy mouse model via the inhibition of Ara h1 secretion, and might be useful for developing functional health foods.

주제어

표/그림 (8)

그림 Fig. 1. Experimental plan of repeated cholera toxin inducement
그림 Fig. 2. Changes in rectal temperature (℃) in cholera toxininduced mouse model.
그림 Fig. 3. Cholera toxin-induced peanut extract and Canavalia gladiata extract inhibiting IL-4, IL-10, and IFN-γ expression in splenocytes of cholera toxin-induced mouse model.
그림 Fig. 4. Total PBMC, splenocytes, and MLN cells in each treatment group of the Canavalia gladiata extract of the cholera toxin-induced mouse model.
그림 Fig. 5. Histopathology of ear tissue (toluidine blue staining) in mast cells of cholera toxin-induced systemic anaphylaxis.
그림 Fig. 6. Effects of CGE treatment on the changes in total absolute numbers of T cells in the PBMC of C57bl/6 mice in the cholera toxin-induced mouse model.
그림 Fig. 7. Effects of CGE treatment on the changes in total absolute numbers of T cells in the MLN of C57bl/6 mice in the cholera toxin-induced mouse model.
그림 Fig.8. Effects of CGE treatment on the changes in total absolute numbers of T cells in the spleen of C57bl/6 mice in the cholera toxin-induced model.

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문제 정의

gladiata, there has been no study to date on its effect on food allergies. Therefore, the objective of the present study was to evaluate the effect of the seeds of C. gladiata extract (CGE) on food allergens.

제안 방법

After sacrificing the C57bl/6 mice, peripheral blood mononuclear cells (PBMC), separated splenocyte cells, and mesenteric lymph node (MLN) cells were centrifuged, and the pellets were used for analysis. Each separated cell was washed twice with FACS buffer and immunofluorescence staining was performed at 4℃.

대상 데이터

Ethanol was purchased from Samchun Chemicals Ltd. (Pyeongtek, Korea); dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO, USA); and Dulbecco’s modified Eagle’s medium, fetal bovine serum (FBS), penicillin-streptomycin B (antibiotics), phosphate buffered saline (PBS), and 0.25% trypsin-EDTA were purchased from Gibco (Carlsbad, CA, USA).
Four-week-old female C57bl/6n mice were supplied from Orient Bio Co., Ltd. and acclimated to an experimental animal room for 1 week. The temperature of the room was adjusted to 20 ± 2℃ and the relative humidity was adjusted to 50 ± 5%.
25% trypsin-EDTA were purchased from Gibco (Carlsbad, CA, USA). The antibodies for fluorescence-activated cell sorting (FACS) analysis (R-Phycoerythrin (PE) anti-mouse fluorescein (FITC) anti-mouse CD4 (RM4-5, rat IgG2a), PE anti-mouse CD8 (53-6.7, rat IgG2a), PE anti-mouse CD69 (H1.2F3, rat IgG2a), FITC anti-mouse CD11b+F4/80+(aM, rat IgG2b), PE-cy5.5 anti-mouse B220 (RA3-6B2, rat IgG2b), and FITC anti-mouse NK1.1 (PK136, rat IgG2b)) were purchased from Biosciences (Franklin Lakes, USA). Mouse interferon gamma, IL-4 and IL-10 (R&D system, USA), and Ara h1-specific IgE enzyme-linked immunosorbent assay (ELISA) (INDOOR biotechnology, Virginia, USA) were also used.

데이터처리

Data were analyzed using the Statistical Package for the Social Sciences (SPSS; version 14.0, SPSS Inc, Chicago, IL, USA) and the significance of each group was p<0.05, which was determined by Duncan’s multiple range test and t-test.

성능/효과

Differences between the cholera toxin-induced group, cholera toxin complex peanut extract administration group, and CGE administration group were confirmed.

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