[논문]비자 유박 추출물이 최종당화산물과 콜라겐 교차결합에 미치는 효과

손다희; 김민경; 박덕훈; 정은선

doi:10.15230/scsk.2019.45.1.19

비자 유박 추출물이 최종당화산물과 콜라겐 교차결합에 미치는 효과
Effect of Defatted Torreya nucifera Seed Extract on the Cross-linking of Advanced Glycation End Products to Collagen 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.45 no.1, 2019년, pp.19 - 25

손다희 (바이오스펙트럼(주) 생명과학연구소) , 김민경 (바이오스펙트럼(주) 생명과학연구소) , 박덕훈 (바이오스펙트럼(주) 생명과학연구소) , 정은선 (바이오스펙트럼(주) 생명과학연구소)

초록
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최종당화산물은 환원당과 단백질, 지질 또는 핵산 사이의 당화반응에 의해서 생성되는 물질로 이물질은 피부 노화 과정에 밀접하게 연관되어 있다고 보고되어 있다. 본 연구에서는 항산화 활성, 최종당화산물 생성 저해, 콜라겐과 최종당화산물의 교차결합 저해 및 억제, 엘라스타제 활성 저해 효능 시험을 통해서 비자나무 유박 추출물의 항노화 효과를 평가하였다. 시험 결과, 비자나무 유박 추출물은 폴리페놀과 플라보노이드를 함유하고 있으며, DPPH와 ABTS 자유라디칼을 50% 소거할 수 있는 농도는 각각 $16.4{\mu}g$ (Dried materials, DM)/mL과 $16.7{\mu}g\;DM/mL$ 로 관찰되었다. 또한, 비자나무 유박 추출물은 농도 의존적으로 최종당화산물 생성과 엘라스타제 활성을 저해하였고, 콜라겐과 최종당화산물의 교차결합을 저해 및 억제하였다. 따라서 본 연구 결과를 바탕으로 비자나무 유박 추출물은 항노화 효능이 있는 화장품 소재로서 활용가치가 있음을 확인하였다.

Abstract ▼ AI-Helper

Advanced glycation end products (AGEs) are final products formed by glycation reaction between reducing sugars and proteins, lipids or nucleic acids. These AGEs are related to progress of skin aging. In this study, we evaluate anti-aging activity of Defatted Torreya nucifera seed extract (DTSE) through antioxidant, anti-glycation, anti-elastase and inhibitory and breaking activity on the cross-linking of AGEs to collagen assay. Results showed that DTSE contained polyphenols and flavonoids. The $IC_{50}$ values of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity were $16.4{\mu}g$ (Dried materials, DM)/mL and $16.7{\mu}g\;DM/mL$, respectively. DTSE also inhibited the formation of AGEs, elastase activity and cross-linking of AGEs to collagen as well as broke existing cross-linking of AGEs to collagen in a dose-dependent manner. Consequently, our findings suggest that DTSE could be useful as a cosmetic material with anti-aging activity.

주제어

표/그림 (5)

표 Table 1. Determination of total polyphenol, flavonoid contents and free radical scavenging activities of Defatted Torreya nucifera seed extract (DTSE). The contents of total polyphenol and flavonoid contents were expressed as gallic acid equivalents (GAE) and rutin equivalents (RE). DM indicates dried materials. The results are mean ± standard deviation (SD) (n=3)
그림 Figure 1. Anti-glycation activity of Defatted Torreya nucifera seed extract (DTSE). Aminoguanidin (AG) was used as a positive control. The results are mean ± standard deviation (SD) (n = 3). * p < 0.05 and ** p < 0.01 vs. Control.
그림 Figure 2. Inhibitory activity of Defatted Torreya nucifera seed extract (DTSE) on the collagen-AGEs cross-linking. Aminoguanidin (AG) was used as a positive control. The results are mean ± standard deviation (SD) (n = 3). * p < 0.05 and ** p < 0.01 vs. Control.
그림 Figure 3. Breaking activity of Defatted Torreya nucifera seed extract (DTSE) on the collagen-AGEs cross-linking. ALT-711 was used as a positive control. The results are mean ± standard deviation (SD) (n = 3). ** p < 0.01 vs. Control.
그림 Figure 4. Inhibitory effect of Defatted Torreya nucifera seed extract (DTSE) on the elastase activity. Selective inhibitor of elastase (SIE), N-methoxysuccinyl-Ala-Ala-Pro-Val -chloromethyl ketone, was used as a positive control. The results are mean ± standard deviation (SD) (n = 3). ** p < 0.01 vs. Control.

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문제 정의

In this study, we demonstrated that DTSE had anti-aging activity through various in vitro assay. DTSE had significant amount of polyphenol and flavonoid as well as strong free radical scavenging activity and showed anti-glycation activity, anti-elastase activity as well as inhibitory and breaking activity on the cross-linking of AGEs to collagen.
While most studies have focused on various activities of its fruits, leaves and seeds, there are no studies about its defatted seed cakes. Therefore, the aim of this study was to investigate biological activities of defatted seed cakes of T. nucifera and to demonstrate its value of use in the cosmetic industry.

제안 방법

According to a previous study, application of ALT-711 increases skin elasticity of aged rats[3]. In this study, we confirmed inhibitory and breaking activity of DTSE on the cross-linking of AGEs to collagen by ELISA. Inhibitory activity of DTSE on the cross-linking was shown in Figure 2.
Especially, accumulation of AGEs in dermal tissue not only lead to oxidative stress in the skin, but also turns skin colors yellow, which is not visually appealing[7]. In this study, we performed anti-glycation assay to confirm inhibitory activity of DTSE on formation of AGEs. As shown in Figure 1, DTSE inhibited formation of AGEs by 5.

대상 데이터

The seeds of T. nucifera were purchased from Jeju island(Korea). 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), L-ascorbic acid, ethanol, bovine serum albumin (BSA), glycolaldehyde, tetramethylbenzidine (TMB) substrate and sulfuric acid(H₂SO₄) were purchased from Sigma (USA).

데이터처리

Statistical significance of data was determined by a Student’s t-test.

이론/모형

Inhibitory and breaking activity of DTSE on the cross-linking of AGEs to collagen were determined by the modified previous method[19]. Briefly, AGEs labeled with horseradish peroxidase (AGEs-HRP) were prepared using a peroxidase labeling kit-NH₂ Unit (Dojindo, Japan).

성능/효과

3. As a result, DTSE broke the cross-linking of AGEs to collagen by 22.1, 30.8, 48.5 and 85.9% at concentrations of 5, 10, 20 and 50 μg/mL, while ALT-711 reduced the cross-linking of AGEs to collagen by 27.2, 37.3, 56.0 and 67.4% at concentrations of 1, 2, 5 and 10 mM, respectively.

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