[논문]광주지역 한우 분변 내 설사병 병원체 조사

고바라다; 김효중; 오아름; 정보람; 박재성; 이재기; 나호명; 김용환

doi:10.7853/kjvs.2019.42.2.93

광주지역 한우 분변 내 설사병 병원체 조사
Prevalence of enteropathogens in the feces from diarrheic Korean native cattle in Gwangju area, Korea 원문보기

韓國家畜衛生學會誌 = Korean journal of veterinary service, v.42 no.2, 2019년, pp.93 - 112

고바라다 (광주광역시보건환경연구원) , 김효중 (광주광역시보건환경연구원) , 오아름 (광주광역시보건환경연구원) , 정보람 (광주광역시보건환경연구원) , 박재성 (광주광역시보건환경연구원) , 이재기 (광주광역시보건환경연구원) , 나호명 (광주광역시보건환경연구원) , 김용환 (광주광역시보건환경연구원)

Abstract ▼ AI-Helper

Calf diarrhea is a common disease in young claves and is still a major cause of productivity and economic loss in livestock farms. Fecal samples from Korean native cattle (n=100) with diarrhea from 64 farms in Gwangju area, Korea from september 2017 to December 2018 were examined for shedding of important protozoan parasitic, viral and bacterial pathogens using culture, rapid test kit and PCR methods. Of 57 (89.1%) of the 64 Korean native cattle farms examined had samples infected with at least one of the investigated pathogens. Among 100 fecal samples, 88 samples were positive for at least one the twelve pathogens and 51 samples were simultaneously positive for two or more pathogens by culture and PCR assay. Bovine group A rotavirus (BRV) was the most common pathogen, found in 43/100 (43.0%) samples on 32/64 (50.0%) farms. Subsequently, kobuvirus (30.0%), pathogenic E. coli (29.0%), bovine parvovirus (17.0%), Giardia spp. (13.0%), Eimeria spp. (10.0%), Clostridium perfringens type A (8.0%), bovine torovirus (8.0%), bovine viral diarrhea virus (6.0%), bovine coronavirus (5.0%), bovine norovirus (2.0%) and Cryptosporidium spp. (2.0%) were detected. Nebovirus, kırklareli virus, bovine adenovirus, Salmonella spp. and intestinal parasites were not detected. Of the 72 calves sampled in this age group, 64 (88.9%) samples were positive for at least one enteropathogen. BRV was identified in 34/72 (47.2%) samples from 27/48 (56.3%) farms. Subsequently, pathogenic E. coli (30.6%), kobuvirus (29.2%), BPaV (22.2%), Giardia spp. (15.3%), Eimeria spp. (9.7%), BVDV (6.9%), Cl. perfringens type A (6.9%), BCoV (4.6%) and Cryptosporidium spp. (2.8%) were detected in fecal samples. A total of ninety-six strains of E. coli were isolated from one hundred fecal samples collected from Korean native cattle with diarrhea. The presence of stx1, stx2, eaeA, LT, STa, STb, ehxA, saa, F4, F5(K99), F6, F17, F18 and F41 genes in the isolates was investigated by PCR. Out of ninety-six E. coli isolates screened for specific genes, 30 strains E. coli were identified to harbor shiga toxin-producing E. coli (STEC) 7 (7.3%), enterohemorrhagic E. coli (EHEC) 8 (8.3%), enteropathogenic E. coli (EPEC) 6 (6.3%), enterotoxigenic E. coli (ETEC) 2 (2.1%) and STEC/ETEC hybrid 7 (7.3%). This study provides epidemiological estimates of the prevalence of Korean native cattle's enteropathogens in Gwangju area, Korea, which would be used for cattle farmers and veterinarians to select appropriate therapeutic method.

주제어

표/그림 (14)

표 Table 1. List of the oligonucleotide primers designed from target genes of the viral enteropathogens used in this study
표 Table 2. Prevalence (%) of enteric pathogens detected in fecal samples from diarrheic Korean native cattle distributed by age group*
표 Table 3. Prevalence (%) of enteric pathogens detected in fecal samples from diarrheic Korean native cattle distributed by season and age group
그림 Fig. 1. Distribution of samples according to the number of enteropathogens detected in one fecal sample (n=100).
그림 Fig. 3. Farm detection rate for enteropathogens detected in Korean native cattle.
그림 Fig. 2. Prevalence of case detected single enteropathogen (n=37).
그림 Fig. 4. Prevalence of fecal samples by months Korean native calves with diarrhea (n=72).
표 Table 4. Prevalence of detected enteropathogens on 64 Korean native cattle farm in Gwangju area, and in fecal samples from cattle (n=100) on those farms
표 Table 5. Simultaneous infection of Korean native cattle with diarrhea by enteric pathogens
표 Table 6. Virulence factors of 96 E. coli strains isolated from diarrheic Korean native cattle
표 Table 7. Distribution of virulence factors of 96 E. coli isolates from Korean native cattle with diarrhea
표 Table 8. Distribution of 96 E. coli by age of Korean native cattle with diarrhea
표 Table 9. Prevalence of 30 pathogenic E. coli by season of Korean native cattle with diarrhea
표 Table 10. Comparative performance of RT-PCR and rapid test kit in detecting BRV and BCoV from fecal samples of diarrheic Korean native cattle

질의응답

핵심어	질문	논문에서 추출한 답변
	송아지 설사의 임상 증상은?	송아지 설사는 어린 송아지에서 흔히 발생하는 질병이며, 축산농가에서 여전히 생산성과 경제적 손실의 주요 원인이 된다. 임상 증상은 수양성 황색 설사,회색 및 녹색 또는 혈액이나 점액이 포함된 설사, 탈수증, 체중감소 및 경련 등이 나타나며, 증상이 심해지면 때로는 폐사에 이른다(Schroeder 등, 2012). 여러 가지 병원체가 송아지 설사를 유발하거나 관여하는 것으로 알려져 있으며, 환경 및 사양관리, 영양 및 면역상태, 병원체 감염정도와 같은 여러 가지 위험요인이 질병의 심각성이나 결과에 영향을 미친다(Cho와 Yoon, 2014).
	송아지 설사란?	송아지 설사는 어린 송아지에서 흔히 발생하는 질병이며, 축산농가에서 여전히 생산성과 경제적 손실의 주요 원인이 된다. 임상 증상은 수양성 황색 설사,회색 및 녹색 또는 혈액이나 점액이 포함된 설사, 탈수증, 체중감소 및 경련 등이 나타나며, 증상이 심해지면 때로는 폐사에 이른다(Schroeder 등, 2012).
	송아지 설사의 전염성 인자의 특징은?	송아지 설사는 전염성 인자와 비전염성 인자 모두에 기인한다(Bartels 등, 2010; Cho와 Yoon, 2014). 전염성 인자로는 바이러스, 세균 및 기생충과 같은 소화기 병원체가 설사 발병과 관련되어 있으며, 단일 감염보다는 복합 감염 형태로 자주 관찰된다(Kang 등, 2001; Izzo 등, 2011). 두 가지 이상의 병원체에 감염된 송아지는 단일 병원체에 감염된 송아지보다 설사증상이 발생할 가능성이 6배정도 더 높다(Boileau와 Kapil, 2010).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
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