[논문]Anti-oxidant activity of avicularin and isovitexin from Lespedeza cuneata

Lee, Ju Sung; Lee, Ah Young; Quilantang, Norman G.; Geraldino, Paul John L.; Cho, Eun Ju; Lee, Sanghyun

doi:10.3839/jabc.2019.020

Anti-oxidant activity of avicularin and isovitexin from Lespedeza cuneata 원문보기

Journal of applied biological chemistry, v.62 no.2, 2019년, pp.143 - 147

Lee, Ju Sung (Department of Plant Science and Technology, Chung-Ang University) , Lee, Ah Young (Department of Food Science, Gyeongnam National University of Science and Technology) , Quilantang, Norman G. (Department of Plant Science and Technology, Chung-Ang University) , Geraldino, Paul John L. (Department of Biology, University of San Carlos) , Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) , Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)

Abstract ▼ AI-Helper

This study evaluated the anti-oxidant activity of avicularin and isovitexin from Lespedeza cuneata using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) scavenging assays. The results showed that among the four fractions, the highest OH radical scavenging activity was exhibited by the n-butanol fraction. The DPPH radical scavenging activities of avicularin and isovitexin were higher than 50% at a concentration of $100{\mu}g/mL$. Moreover, one hundred micrograms per liter of avicularin and isovitexin exhibited effective anti-oxidant activity, with the OH radical scavenging rates being 87.54 and 91.48%, respectively. These results suggest that Lespedeza cuneata would be useful anti-oxidant agents from natural sources.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

cuneata in Gokseong for obtaining maximum yields of avicularin and isovitexin. This study can further improve the development of functional foods with high anti-oxidant effects.

제안 방법

cuneata by analyzing four solvent fractions of the plant. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical scavenging activities were measured to evaluate the anti-oxidant ability of these fractions. The average contents of avicularin and isovitexin in the samples collected from different geographical regions in Korea were simultaneously detected by using high-performance liquid chromatography (HPLC).

대상 데이터

The 1290 Infinity II LC system with INNO C18 column (250 mm×4.6 mm, 5 µm) from Agilent Technology was used for the chemical analyses.
cuneata were collected from Gokseong, Inje, and Boeun, in Korea in the month of October in 2016 and in the month of November in 2017. The voucher specimens were identified by Prof. S. S. Roh, Daegu Haany University and deposited at the herbarium of the Department of Plant Science and Technology, Chung-Ang University, Anseong, Korea. Dried samples of L.

데이터처리

Significant differences in the values measured during the experiments were determined using Duncan’s Multiple Range Test using the SAS software (version 6.0, SAS Institute, Cary, NC, USA).

성능/효과

Based on our results, the n-BuOH fraction of L. cuneata, which contains higher amounts of flavonoids than the other fractions, exhibited the most effective free radical scavenging activity. The anti-oxidant activity of the n-BuOH fraction can be attributed to the compounds avicularin and isovitexin.
The anti-oxidant activity of the n-BuOH fraction can be attributed to the compounds avicularin and isovitexin. This study also established that the month of October is a better time for harvesting L. cuneata in Gokseong for obtaining maximum yields of avicularin and isovitexin. This study can further improve the development of functional foods with high anti-oxidant effects.

후속연구

However, our results indicated that both avicularin and isovitexin have anti-oxidant properties possibly because they can both act as hydrogen donors and/or metal ion chelators [3]. This study further supports this claim and highlights the use of the nBuOH fraction for future studies on the anti-oxidant properties of L. cuneata.

참고문헌 (24)

Holliwell B, Gutteridge JM, Cross CE (1992) Free radicals anti-oxidants, and human disease:Where are we now?. J Lab Clin Med 119: 598-620.
Hertog, M.G.L., Feskens, E.J.M., Kromhout, D., Hertog, M.G.L., Hollman, P.C.H., Hertog, M.G.L., Katan, M.B.. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. The Lancet, vol.342, no.8878, 1007-1011.

상세보기
Yoo, Guijae, Park, Seon Ju, Lee, Taek Hwan, Yang, Heejung, Baek, Yoon-su, Kim, Nanyoung, Kim, Yoon Jae, Kim, Seung Hyun. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia cells. Pharmacognosy magazine, vol.11, no.43, 651-656.

상세보기
김현영, 고지연, 송석보, 김정인, 서혜인, 이재생, 곽도연, 정태욱, 김기영, 오인석, 정헌상, 우관식. 비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 α-Glucosidase 저해활성. 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, vol.41, no.11, 1508-1514.

원문보기 상세보기
Nam, Jin-Sik, Park, Seo-Yeon, Jang, Hye-Lim, Rhee, Young Ha. Phenolic compounds in different parts of young Annona muricata cultivated in Korea and their antioxidant activity. Applied Biological Chemistry = 한국응용생명화학회지, vol.60, no.5, 535-543.

상세보기
Lee, Jaemin, Rodriguez, Joyce P., Lee, Kang Hee, Park, Jun Yeon, Kang, Ki Sung, Hahm, Dae-Hyun, Huh, Chang Ki, Lee, Sang Cheon, Lee, Sanghyun. Determination of flavonoids from Cirsium japonicum var. maackii and their inhibitory activities against aldose reductase. Applied Biological Chemistry = 한국응용생명화학회지, vol.60, no.5, 487-496.

상세보기
Williams, Robert J, Spencer, Jeremy P.E, Rice-Evans, Catherine. Flavonoids: antioxidants or signalling molecules?. Free radical biology & medicine, vol.36, no.7, 838-849.

상세보기
Choi JS, Cho CS, Kim CJ (2010) Cytoprotective effect of Lespedeza cuneata extract on glucose toxicity. J Korean Oriental Med 31: 79-100.
이호일, 정지윤, 황보민, 구세광, 김영우, 지선영. 야관문(夜關門)의 항염증효과(抗炎症效果). 大韓本草學會誌 = The Korea journal of herbology, vol.28, no.4, 83-92.

원문보기 상세보기
Kim, Min Suk, Sharma, Bhesh Raj, Rhyu, Dong Young. Beneficial Effect of Lespedeza cuneata (G. Don) Water Extract on Streptozotocin-induced Type 1 Diabetes and Cytokine-induced Beta-cell Damage. Natural product sciences, vol.22, no.3, 175-179.

원문보기 상세보기
Kwon DJ, Kim JK, Ham YH, Bae YS (2007) Flavone glycosides from the aerial parts of Lespedeza cuneata G. Don. J Korean Soc Appl Biol Chem 50: 344-347.
Kwon, D.J., Bae, Y.S.. Flavonoids from the aerial parts of Lespedeza cuneata. Biochemical systematics and ecology, vol.37, no.1, 46-48.

상세보기
Kim, Sang Min, Kang, Kyungsu, Jho, Eun Hye, Jung, Yu‐Jin, Nho, Chu Won, Um, Byung‐Hun, Pan, Cheol‐Ho. Hepatoprotective Effect of Flavonoid Glycosides from Lespedeza cuneata against Oxidative Stress Induced by tert‐Butyl Hyperoxide. Phytotherapy research : PTR, vol.25, no.7, 1011-1017.

상세보기
Kim, Jeong Ah, Jung, Yi‐Sook, Kim, Mi‐Young, Yang, Seo Young, Lee, Sohyun, Kim, Young Ho. Protective Effect of Components Isolated from Lindera erythrocarpa against Oxidative Stress‐induced Apoptosis of H9c2 Cardiomyocytes. Phytotherapy research : PTR, vol.25, no.11, 1612-1617.

상세보기
He, M., Min, J.W., Kong, W.L., He, X.H., Li, J.X., Peng, B.W.. A review on the pharmacological effects of vitexin and isovitexin. Fitoterapia, vol.115, 74-85.

상세보기
Kwon, A-Reum, Oh, Myung-Min, Paek, Kee-Yoeup, Park, So-Young. The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis ‘Spring Dancer’. Horticulture, Environment, and Biotechnology, vol.59, no.2, 179-188.

상세보기
Nam, Tae-Gyu, Lim, You Jin, Eom, Seok Hyun. Flavonoid accumulation in common buckwheat (Fagopyrum esculentum) sprout tissues in response to light. Horticulture, Environment, and Biotechnology, vol.59, no.1, 19-27.

상세보기
Baek, Jiwon, Lee, Tae Kyoung, Song, Jae-Hyoung, Choi, Eunyong, Ko, Hyun-Jeong, Lee, Sanghyun, Choi, Sang Un, Lee, Seong, Yoo, Sang-Woo, Kim, Seon-Hee, Kim, Ki Hyun. Lignan Glycosides and Flavonoid Glycosides from the Aerial Portion of Lespedeza cuneata and Their Biological Evaluations. Molecules a journal of synthetic chemistry and natural product chemistry, vol.23, no.8, 1920-.

상세보기
HATANO, Tsutomu, EDAMATSU, Rei, HIRAMATSU, Midori, MORI, Akitane, FUJITA, Yuzaburo, YASUHARA, Taeko, YOSHIDA, Takashi, OKUDA, Takuo. Effects of the interaction of tannins with Co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical.. Chemical & pharmaceutical bulletin, vol.37, no.8, 2016-2021.

상세보기
Chung, Shin-Kyo, Osawa, Toshihiko, Kawakishi, Shunro. Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard (Brassica nigra). Bioscience, biotechnology, and biochemistry, vol.61, no.1, 118-123.

상세보기
Aruoma, O.I.. Nutrition and health aspects of free radicals and antioxidants. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, vol.32, no.7, 671-683.

상세보기
Kim JS, Kim MJ (2010) In vitro anti-oxidant activity of Lespedeza cuneata methanolic extracts. J Med Plants Res 2: 674-679.
Heim, Kelly E, Tagliaferro, Anthony R, Bobilya, Dennis J. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of nutritional biochemistry, vol.13, no.10, 572-584.

상세보기
Seyoum, Ameha, Asres, Kaleab, El-Fiky, Fathy Kandeel. Structure–radical scavenging activity relationships of flavonoids. Phytochemistry, vol.67, no.18, 2058-2070.

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증